干旱区生态环境知识资源中心(Arid): 干热环境创伤失血性休克猪继发肝损伤的变化特点及机制

Arid

	干热环境创伤失血性休克猪继发肝损伤的变化特点及机制
其他题名	Characteristics and mechanism of secondary liver injury in pigs with traumatic hemorrhagic shock in dry heat environment
	吴彬; 游云; 沈才福; 夏亮; 杨欣悦; 李辉文; 刘江伟; 戴勇华
来源期刊	解放军医学杂志
ISSN	0577-7402
出版年	2021
卷号	46 期号:3 页码:0577-7402
中文摘要	目的探讨模拟沙漠干热环境下创伤失血性休克(THS)猪继发肝损伤的变化特点及机制。方法60只雄性长白仔猪随机分为干热创伤失血性休克组(DHS组)、干热创伤失血性休克假手术组(DHC组)与常温创伤失血性休克组(NTS组),每组20只。将DHS组、DHC组于干热环境[温度(40.50.5) ℃,湿度10%2%],NTS组于常温环境[温度(25.00.5) ℃,湿度35%5%]下分别暴露3 h,然后建立血压控制型失血性休克模型。各组分别于模型建立成功后0 min(T_0)、50 min(T_1)、100 min(T_2)、150 min(T_3)安乐处死,收集血液和肝组织。HE染色观察肝组织病理学变化,ELISA法检测肝组织TNF-alpha、IL-1beta水平,Western blotting检测肝组织HMGB-1、ICAM-1的表达;测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)含量。结果HE染色结果显示,DHS组各时间点均有不同程度的损伤,且随着时间延长逐渐加重,并逐渐出现不同程度的肝窦扩张、充血,肝小叶、汇管区结构稍紊乱,炎性细胞浸润,肝细胞变性、坏死等。NTS组于T_2时开始出现肝损伤并逐渐加重,DHC组各时间点肝组织均未出现明显的病理学变化。DHS组ALT及AST均呈动态变化且趋势基本一致,与DHC组比较,T_0时即开始升高,差异有统计学意义(P<0.01),分别在T_1、T_3时达到峰值,且与同时间点DHC组、NTS组比较差异有统计学意义(P<0.01);而DHC组在各时间点均未出现明显变化(P>0.05)。DHS组TNF-alpha、IL-1beta水平均在T_0时即开始升高,且与相同时间点DHC组、NTS组比较差异有统计学意义(P<0.01),随时间推移DHS组与NTS组均呈现持续增高趋势,且DHS组增高速度较NTS组更快,差异有统计学意义(P<0.01),而DHC组未见明显升高(P>0.05);随时间延长,DHS组ICAM-1、HMGB-1表达水平较前一时间点逐渐升高(P<0.05或P<0.01)。结论干热环境创伤失血性休克猪继发肝损伤出现时间早且重,可激活肝内更多的Kupffer细胞,促使TNF-alpha、IL-1beta大量释放并进一步引起HMGB-1、ICAM-1高表达,共同促进肝组织损伤。
英文摘要	Objective To document the dynamics of secondary liver injury in pigs with traumatic hemorrhagic shock(THS) induced by simulated desert dry heat environment and to explore its possible mechanism.Methods Sixty male Landrace piglets were randomly divided into three groups:Dry heat trauma hemorrhagic shock group (DHS group,n=20),dry heat trauma hemorrhagic shock sham operation group (DHC group,n=20),normal temperature trauma hemorrhagic shock group (NTS group,n=20).DHS group and DHC group were exposed to a dry and hot environment [temperature (40.50.5) ℃,humidity 10%2%];while NTS group were exposed to normal temperature environment [temperature (25.00.5) ℃,humidity 35%5%] for 3 h.Then the model of blood pressure controlled hemorrhagic shock was established.Animals were euthanized at 0 min (T_0),50 min(T_1),100 min (T_2) or 150 min (T_3) after the successful establishment of the hemorrhagic shock model,blood and live tissue were collected.The pathological changes of liver tissue were observed by HE staining;the levels of TNF-alpha and IL-1beta in liver tissue were detected by ELISA;the expressions of HMGB-1 and ICAM-1 in liver tissue were detected by Western blotting;the contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were determined.Results HE staining results showed that the DHS group had different degrees of injury at each time point,and gradually aggravated with time,and gradually appeared different degrees of hepatic sinusoidal expansion,congestion,hepatic lobule,portal area structure disorder,inflammatory cell infiltration,liver cell degeneration,necrosis and so on.In the NTS group,the injury began to appear at T_2 and gradually worsened.In the DHC group,there was no obvious pathological change at each time point (P>0.05).In the DHS group,ALT and AST showed dynamic changes,and the changing trend was the same,compared with the DHC group,it began to rise at T_0,the difference was statistically significant (P<0.01),and reached the peak at T_1 and T_3,respectively,and the differences were statistically significant compared with DHC group and NTS group at the same time point (P<0.01);however,there was no significant change in DHC group at each time point.TNF-alpha and IL-1beta began to increase at T_0 in the DHS group,and the differences were statistically significant compared with the DHC group and NTS group at the same time point (P<0.01).Both the DHS group and NTS group showed a continuous growth trend in a time-dependent manner with a significantly faster growth rate in the DHS group than that of the NTS group (P<0.01).There were no statistical significances on ALT or AST levels in the DHC group (P>0.05).As time went on,the expression of ICAM-1 and HMGB-1 in the DHS group was higher than that in the previous time point (P<0.05 or P<0.01).Conclusion In desert dry and hot environment,the secondary liver injury of THS occurs early and progresses seriously.This can activate more Kupffer cells in the liver to promote the release of TNF-alpha and IL-1beta leading to elevated expression of HMGB-1 and ICAM-1 resulting in further liver injury.
中文关键词	创伤失血性休克 ; 干热环境 ; 继发肝损伤 ; 炎性因子 ; 细胞间黏附分子-1 ; 高迁移率族蛋白B-1
英文关键词	traumatic hemorrhagic shock dry heat environment secondary liver injury inflammatory factors intercellular cell adhesion molecule-1 high mobility group box-1
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	General & Internal Medicine
CSCD记录号	CSCD:6949358
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/354349
作者单位	吴彬, 新疆特殊环境医学重点实验室;;解放军联勤保障部队临潼康复疗养中心, 新疆特殊环境医学重点实验室;;, 乌鲁木齐;;西安, ;; 830000;;710000.; 游云, 新疆特殊环境医学重点实验室, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国.; 夏亮, 新疆特殊环境医学重点实验室, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国.; 杨欣悦, 新疆特殊环境医学重点实验室, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国.; 刘江伟, 新疆特殊环境医学重点实验室, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国.; 戴勇华, 新疆特殊环境医学重点实验室, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国.; 沈才福, 新疆特殊环境医学重点实验室;;陆军957医院消化血液内分泌科, 新疆特殊环境医学重点实验室;;, 乌鲁木齐;;阿里, ;;西藏 830000;;859000.; 李辉文, 解放军69223部队卫生连, 阿克苏, 新疆 842300, 中国.
推荐引用方式 GB/T 7714	吴彬,游云,沈才福,等. 干热环境创伤失血性休克猪继发肝损伤的变化特点及机制[J],2021,46(3):0577-7402.
APA	吴彬.,游云.,沈才福.,夏亮.,杨欣悦.,...&戴勇华.(2021).干热环境创伤失血性休克猪继发肝损伤的变化特点及机制.解放军医学杂志,46(3),0577-7402.
MLA	吴彬,et al."干热环境创伤失血性休克猪继发肝损伤的变化特点及机制".解放军医学杂志 46.3(2021):0577-7402.