干旱区生态环境知识资源中心(Arid): 荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响

Arid

	荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响
其他题名	Effects of the Alfalfa Post-harvest Rotation on Soil Bacterial Community in Arid Desert Oasis
	南丽丽; 郭全恩; 刘雪强
来源期刊	草地学报
ISSN	1007-0435
出版年	2021
卷号	29 期号:1 页码:25-32
中文摘要	为阐明荒漠灌区苜蓿(Medicago sativa)后茬轮作不同作物对土壤细菌群落的影响,本研究以6年生苜蓿地为对照(CK),以5年生苜蓿地翻耕灭茬分别种植1年小麦(Triticum aestivum)(T_1)、油菜(Brassica chinensis)(T_2)、葵花(Helianthus annuus)(T_3)、玉米(Zea mays)(T_4)为处理,采用高通量测序技术,分析不同处理下土壤细菌群落的变化特征。结果表明,T_1,T_2微生物量碳含量显著大于CK,T_4 微生物量氮含量显著高于CK(P<0.05); T_3,T_4土壤呼吸显著大于CK(P<0.05);T_1,T_2,T_3,T_4脲酶、碱性磷酸酶、过氧化氢酶、蔗糖酶活性分别是CK的1.44, 1.13,1.36,1.54倍,1.53,7.78,4.25,7.28倍,1.09,1.28,1.14,1.06倍,1.11,1.38,1.14 ,1.74倍;与CK相比,轮茬后变形菌门、放线菌门、芽单胞菌门相对丰富呈降低、而酸杆菌门相对丰富呈增加趋势,这4个菌门为不同处理的优势菌门,占所有菌门的82.67%;T_1,T_2土壤细菌群落丰富度指数(Chao1和ACE)均显著高于CK(P< 0.05)。综上,荒漠灌区轮茬较苜蓿连作更有助于提高土壤酶活性,稳定细菌群落结构,提高土地生产力。
英文摘要	In order to determine the effects of alfalfa post-harvest rotation on soil bacterial community in arid desert oasis,an experiment was set up with the following five crop rotation treatments:(Ⅰ)control (CK)with six-year-old alfalfa;(Ⅱ)5-year-old alfalfa-1-year-old wheat(T_1);(Ⅲ)5-year-old alfalfa-1-yearold canola(T_2);(Ⅳ)5-year-old alfalfa-1-year-old sunflower(T_3);and(Ⅴ)5-year-old alfalfa-1-year-old corn(T_4).The characteristics of soil bacteria in different treatments were analyzed by high-throughput sequencing( Illumina HiSeq).The results showed that T_1and T_2treatments significantly increased soil microbial biomass carbon content.T_4treatment significantly increased soil microbial biomass nitrogen content. T_3and T_4treatments significantly improved soil respiration.Soil urease,alkaline phosphatase,catalase,sucrase activities in T_1,T_2,T_3,and T_4treatments were 1.44,1.13,1.36,and 1.54times,1.53,7.78,4.25,and 7.28times,1.09,1.28,1.14,and 1.06times,1.11,1.38,1.14,and 1.74times higher than that in CK treatment,respectively.Proteobacteria,Acidobacteria,Gemmatimonadetes,and Bacteroidetes were the four dominant bacteria in different treatments,accounting for 82.67%of all bacteria.Compared with CK,Proteobacteria,Gemmatimonadetes,and Actinobacteria decreased while Acidobacteria increased after different crop rotations.The abundance index (ACE and Chao1)of T_1and T_2treatments were significantly higher than CK (P<0.05).In conclusion,different crop rotations increased soil enzyme activities,stabilized bacterial community structure and improved soil productivity.
中文关键词	苜蓿 ; 轮茬 ; 细菌群落结构 ; 高通量测序 ; 荒漠灌区
英文关键词	Alfalfa Rotation Bacterial community structure High throughtput sequencing Arid desert oasis
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6925574
来源机构	甘肃农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/354162
作者单位	南丽丽, 甘肃农业大学草业学院, 兰州, 甘肃 730070, 中国. 郭全恩, 甘肃农业大学草业学院, 兰州, 甘肃 730070, 中国. 刘雪强, 甘肃农业大学草业学院, 兰州, 甘肃 730070, 中国.
推荐引用方式 GB/T 7714	南丽丽,郭全恩,刘雪强. 荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响[J]. 甘肃农业大学,2021,29(1):25-32.
APA	南丽丽,郭全恩,&刘雪强.(2021).荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响.草地学报,29(1),25-32.
MLA	南丽丽,et al."荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响".草地学报 29.1(2021):25-32.