干旱区生态环境知识资源中心(Arid): 固相萃取-超高效液相色谱-串联质谱法同时分析多种单花蜜中黄酮类成分

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	固相萃取-超高效液相色谱-串联质谱法同时分析多种单花蜜中黄酮类成分
其他题名	Determination of flavonoids in honey by solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry method
	沈葹; 王晶波; 陈曦; 刘婷婷; 马妍; 王丽媛; 秦文; 宫照龙; 卓勤
来源期刊	卫生研究
ISSN	1000-8020
出版年	2020
卷号	49 期号:2 页码:259-266
中文摘要	目的建立基于固相萃取-超高效液相色谱-串联质谱法测定蜂蜜中20种黄酮类成分的方法,并应用于多种不同蜜源单花蜜中黄酮类成分的差异研究。方法蜂蜜样品经水溶液超声提取,14 000 * g离心5 min,取上清液经MAX固相萃取柱净化,采用Waters ACQUITY UPLC HSS T3( 2.1 mm * 100 mm,1.8 mum)色谱柱分离,以0.1%甲酸水溶液和0.1%甲酸乙腈溶液作为流动相梯度洗脱,电喷雾电离,在多反应监测模式下检测,外标法定量。结果20种目标化合物线性范围宽、线性关系良好,相关系数均大于0.9969;在100、250和500 mug /L 3个加标水平下方法加标回收率为76.2% ~ 108.6%;日内精密度为0.50% ~ 4.34%;日间精密度为2.51% ~6.71%( n = 6) 。16种不同蜜源单花蜜中黄酮类化合物分布具有明显差异,其中小茴香蜂蜜中槲皮苷含量最高( 165 ng /g); 红桉树蜂蜜( 186 ng /g)和麦卢卡蜂蜜( 304 ng /g)中木犀草素含量高;荞麦蜂蜜、小茴香蜂蜜、麦卢卡蜂蜜和雪脂莲蜂蜜中山奈酚含量较高( 113 ~ 257 ng /g); 雪脂莲蜂蜜、麦卢卡蜂蜜、向日葵蜂蜜和薰衣草蜂蜜中白杨素含量较高( 136 ~ 213 ng /g), 麦卢卡蜂蜜中生松素( 473 ng /g)和短叶松素( 684 ng /g)远高出其它来源单花蜜。结论该方法具有前处理简便、测定灵敏、准确、重现性好的特点,适用于各类蜂蜜中黄酮类物质的定量分析,为单花蜜的差异分析与质量评价建立了更多科学数据与应用基础。
英文摘要	OBJECTIVE To establish a method for simultaneous determination of 20 flavonoid compounds from honey samples using solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry ( UPLC-MS /MS ), which was applied to study the difference of flavonoid compounds in honey samples from different botanical origins. METHODS The honey samples were extracted with ultrawater solution by ultrasound,and purified with an Oasis MAX column,and then separated on a Waters ACQUITY UPLC HSS T3 ( 2.1 mm * 100 mm, 1.8 mum) and eluted with the mobile phase consisted of 0.1% formic acid in water and acetonitrile in a gradient program,and analyzed by mass spectrometry equipped with an electrospray ionization source,operating in the positive or negative ionization modes,and performed in the multiple reaction monitoring using external standards. RESULTS Twenty flavonoid compounds showed good linearity with coefficients higher than 0.9969; The recoveries of spiked honey samples in the range of 76.2% - 108.6% at the concentrations of 100, 250 and 500 mug /L; The relative standard deviations were 0.50% - 4.34% and 2.51% - 6.71%( n = 6) for inter-day and intra-day precisions,respectively. It was found that the variations of flavonoid compounds in 16 types of honey samples from different botanical origins differed largely. Among them,quercetrin content in fennel honey was the highest ( 165 ng /g). High contents of luteolin in red eucalyptus and manuka honey were 186 ng / g and 304 ng /g,respectively. High contents of kaempferol in buckwheat,fennel and manuka honeys were from 113 to 257 ng /g. High contents of chrysin in snow lotus honey, manuka,sunflower honey and lavender Honey were from 136 to 213 ng /g. The content of pinocembrin ( 473 ng /g) and pinobanksin ( 684 ng /g) in manuka honey were much higher than that in other honeys. CONCLUSION The proposed method is simple for pretreatment,also sensitive,accurate and producible for quantitative of 20 flavonoid compounds in different honey samples,which provide more scientific data and application basis for quality analysis.
中文关键词	固相萃取 ; 超高效液相色谱-串联质谱法 ; 蜂蜜 ; 黄酮类化合物
英文关键词	solid phase extraction UPLC-MS /MS honey flavonoid compound
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Public, Environmental & Occupational Health
CSCD记录号	CSCD:6706483
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353905
作者单位	沈葹, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 王晶波, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 陈曦, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 刘婷婷, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 马妍, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 王丽媛, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 秦文, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 宫照龙, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国. 卓勤, 中国疾病预防控制中心营养与健康所, 国家卫生健康委员会微量元素重点实验室, 北京 100050, 中国.
推荐引用方式 GB/T 7714	沈葹,王晶波,陈曦,等. 固相萃取-超高效液相色谱-串联质谱法同时分析多种单花蜜中黄酮类成分[J],2020,49(2):259-266.
APA	沈葹.,王晶波.,陈曦.,刘婷婷.,马妍.,...&卓勤.(2020).固相萃取-超高效液相色谱-串联质谱法同时分析多种单花蜜中黄酮类成分.卫生研究,49(2),259-266.
MLA	沈葹,et al."固相萃取-超高效液相色谱-串联质谱法同时分析多种单花蜜中黄酮类成分".卫生研究 49.2(2020):259-266.