干旱区生态环境知识资源中心(Arid): Cr(VI)还原菌的筛选、鉴定及其还原物质分析

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	Cr(VI)还原菌的筛选、鉴定及其还原物质分析
其他题名	Screening and identification of Cr(VI) reducing bacteria
	韩剑宏; 宋玉艳; 张铁军; 姜庆宏; 张连科; 王维大
来源期刊	微生物学通报
ISSN	0253-2654
出版年	2020
卷号	47 期号:10 页码:3206-3215
中文摘要	【背景】铬污染土壤是我国土壤污染修复的重点治理对象,在众多修复技术中,微生物法因具有简单、经济、无二次污染等特性已成为研究热点,而微生物法中筛选出既能适应污染场地环境又能高效还原Cr(VI)的菌株尤为重要。【目的】筛选适应西北寒旱区高效还原Cr(VI)的菌株,丰富铬还原菌资源库,为铬污染土壤修复奠定基础。【方法】采用富集驯化、分离纯化法进行筛菌;通过形态学和分子生物学相结合的方法对目的菌株进行鉴定;采用傅里叶变换红外光谱法对还原机理进行研究。【结果】菌株G-13有较强的Cr(VI)还原能力,pH 9.0、温度为30 ℃条件下,60 h对Cr(VI)(100 mg/L)的还原率达到82.8%。经形态学和分子生物学鉴定,菌株G-13为Micrococcus luteus。反应中Cr(VI)的降低伴随着Cr(III)的增加,说明以还原反应为主,并且还原能力与细菌生长呈依赖型关系。对细胞各组分及变性研究表明,胞外酶在还原反应中占主要作用。除Pd~(2+)、Cd~(2+)外,其余金属离子对酶活性无明显抑制作用。通过傅里叶变换红外光谱分析,发现G- 13与Cr(VI)结合位点主要为羟基、羰基、羧基、-CH、酰胺基等。【结论】菌株G-13有较强的Cr(VI)还原能力,能为西北寒旱区铬污染土壤修复丰富菌种资源。
英文摘要	[Background] Chromium-contaminated soil is a key target for soil pollution remediation in China. In many remediation technologies, microbial method has become a research hotspot because of its simple, economical, and no secondary pollution. It is particularly important to screen strains that can adapt to the polluted site environment and efficiently reduce Cr(VI) in the microbial method. [Objective] Screening strains adapted to the efficient reduction of Cr(VI) in cold and arid regions of Northwest China, enrich the resource pool of chromium reducing bacteria, and lay the foundation for remediation of chromium-contaminated soil. [Methods] Screening bacteria by enrichment, domestication, isolation and purification; the target strain was identified by a combination of morphology and molecular biology; and the reduction mechanism was studied by fourier transform infrared spectroscopy. [Results] g~(-1)3 has strong Cr(VI) reducing ability. At pH 9.0 and temperature 30 ℃, the reduction rate reached 82.8% in a 100 mg/L Cr(VI) solution within 60 hours. Morphological and molecular biological identification showed that the strain g~(-1)3 was Micrococcus luteus. The decrease of Cr(VI) in the reaction was accompanied by the increase of Cr(III), which indicated that the reduction reaction was the main factor, and the reducing ability was dependent on the growth of bacteria. Studies on cell components and denaturation showed that extracellular enzymes play a major role in the reduction reaction. Except for Pd~(2+) and Cd~(2+), the other metal ions had no significant inhibitory effect on enzyme activity. Through fourier transform infrared spectroscopy analysis, it was found that the binding sites of g-13 and Cr(VI) were mainly -OH, C= O, -COOH, -CH, -CONH_2 and so on. [Conclusion] g-13 has strong Cr(VI) reducing ability, and can be used to rich bacterial resources for repairing chromium-contaminated soil in northwestern cold and arid regions.
中文关键词	铬还原菌 ; 藤黄微球菌 ; 还原特性 ; 傅里叶变换红外光谱
英文关键词	Chromium reduction strains Micrococcus luteus Reduction characteristics Fourier transform infrared spectroscopy
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Environmental Sciences & Ecology
CSCD记录号	CSCD:6824768
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353901
作者单位	韩剑宏, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国. 宋玉艳, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国. 张铁军, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国. 姜庆宏, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国. 张连科, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国. 王维大, 内蒙古科技大学能源与环境学院, 包头, 内蒙古 014010, 中国.
推荐引用方式 GB/T 7714	韩剑宏,宋玉艳,张铁军,等. Cr(VI)还原菌的筛选、鉴定及其还原物质分析[J],2020,47(10):3206-3215.
APA	韩剑宏,宋玉艳,张铁军,姜庆宏,张连科,&王维大.(2020).Cr(VI)还原菌的筛选、鉴定及其还原物质分析.微生物学通报,47(10),3206-3215.
MLA	韩剑宏,et al."Cr(VI)还原菌的筛选、鉴定及其还原物质分析".微生物学通报 47.10(2020):3206-3215.