干旱区生态环境知识资源中心(Arid): PRIME HLB固相萃取-超高效液相色谱-串联质谱法测定蜂产品中10种头孢类药物的残留量

Arid

	PRIME HLB固相萃取-超高效液相色谱-串联质谱法测定蜂产品中10种头孢类药物的残留量
其他题名	Determination of Ten Cephalosporin Residues in Bee Products by Ultra-high Performance Liquid Chromatography- Tandem Mass Spectrometry Coupled with PRIME HLB Solid Phase Extraction
	章豪; 吴银良; 张宜文; 陈国; 江潇潇
来源期刊	食品科学
ISSN	1002-6630
出版年	2020
卷号	41 期号:22
中文摘要	建立蜂产品中10种头孢类药物(头孢喹肟、头孢噻肟、头孢洛宁、头孢哌酮、头孢匹林、头孢氨苄、头孢乙腈、头孢拉定、去乙酰基头孢匹林、头孢唑啉)残留量的超高效液相色谱-串联质谱测定方法。蜂产品样品中头孢类药物用10%甲酸溶液提取,离心,上清液经Oasis PRIME HLB固相萃取柱净化,氮吹后复溶,进行超高效液相色谱-串联质谱分析。采用Acquity BEH C_(18)色谱柱,以0.1%甲酸溶液-甲醇体系作为流动相进行梯度洗脱,电喷雾离子源正离子模式,多反应离子监测模式检测,基质校准外标法定量。结果表明,10种头孢类药物在一定质量浓度范围内相关系数(r2)大于0.999,线性关系良好;其中检出限为0.1~1 mug/kg,定量限为0.3~3 mug/kg;阴性蜂产品样品的加标回收率为80.0%~95.5%,相对标准偏差为1.5%~4.8%。该方法检测周期短,准确度和精密度高,能满足多种蜂产品样品中头孢类药物的检测需要。
英文摘要	An ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantitative determination of residues of 10 cephalosporins in bee products, including honey, royal jelly and lyophilized royal jelly powder, was established. The samples were extracted with 10% formic acid solution. After being purified by solid-phase microextraction using an Oasis PRIME HLB cartridge, the extract was blown to dryness under a stream of nitrogen gas and then re-dissolved. The analytes were separated on an Acquity UPLC BEH C_(18) column with a mixture of 0.1% formic acid solution and methanol as the mobile phase by gradient elution. The analysis was carried out using a positive electrospray ion source in the multiple reaction monitoring mode. The matrix-matched external standard method was applied for the quantitative analysis. There were good linear relationships for 10 cephalosporins in certain concentration ranges, with correlation coefficients of more than 0.999. The limits of detection (LODs) for 10 cephalosporins were 0.1-1 mug/kg, and the limits of quantitation (LOQs) were 0.3-3 mug/kg. The recoveries for the analytes spiked into blank bee products were in the range of 80.0%-95.5%, with relative standard deviations of 1.5%-4.8%. This method was time-saving and suitable for the determination of cephalosporins in different bee products due to its simplicity and reliability.
中文关键词	蜂产品 ; 头孢类药物 ; 超高效液相色谱-串联质谱法 ; Oasis PRIME HLB固相萃取柱
英文关键词	bee products cephalosporins ultra-high performance liquid chromatography-tandem mass spectrometry Oasis PRIME HLB cartridge
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6855648
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353822
作者单位	章豪, 宁波市农业科学研究院, 农业农村部农产品质量安全风险评估实验室(宁波), 宁波, 浙江 315040, 中国. 吴银良, 宁波市农业科学研究院, 农业农村部农产品质量安全风险评估实验室(宁波), 宁波, 浙江 315040, 中国. 陈国, 宁波市农业科学研究院, 农业农村部农产品质量安全风险评估实验室(宁波), 宁波, 浙江 315040, 中国. 江潇潇, 宁波市农业科学研究院, 农业农村部农产品质量安全风险评估实验室(宁波), 宁波, 浙江 315040, 中国. 张宜文, 北京市计量检测科学研究院, 北京 100012, 中国.
推荐引用方式 GB/T 7714	章豪,吴银良,张宜文,等. PRIME HLB固相萃取-超高效液相色谱-串联质谱法测定蜂产品中10种头孢类药物的残留量[J],2020,41(22).
APA	章豪,吴银良,张宜文,陈国,&江潇潇.(2020).PRIME HLB固相萃取-超高效液相色谱-串联质谱法测定蜂产品中10种头孢类药物的残留量.食品科学,41(22).
MLA	章豪,et al."PRIME HLB固相萃取-超高效液相色谱-串联质谱法测定蜂产品中10种头孢类药物的残留量".食品科学 41.22(2020).