干旱区生态环境知识资源中心(Arid): 基于高通量测序的几种不同作物根际土壤细菌群落结构和多样性分析

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	基于高通量测序的几种不同作物根际土壤细菌群落结构和多样性分析
其他题名	Analysis of Bacterial Community Structure and Diversity in Rhizosphere Soil of Several Different Crops Based on High-throughput Sequencing
	孙倩; 吴宏亮; 陈阜; 康建宏
来源期刊	农业生物技术学报
ISSN	1674-7968
出版年	2020
卷号	28 期号:8 页码:1490-1498
中文摘要	宁夏扬黄灌区作物种类单一,在生产中需要配置不同种类的作物以增加其生物多样性。为了探明不同作物对农田土壤细菌群落结构和多样性的影响,本研究以谷子( Setaria italica)、黑豆(Glycine max)、藜麦(Chenopodium quinoa)及休闲地土壤为对象,测定了土壤pH、全氮、有机质、速效钾、速效磷和碱解氮的含量,在此基础上,利用Illumina MiSeq高通量测序技术对比研究了其中的细菌群落结构。结果表明:种植藜麦后土壤有机质、碱解氮、速效钾和速效磷的含量均升高,分别比休闲地高出2.8 8%、19.12%、 112.04%和39.90%,但pH值降低的最少,为0.01。5组土壤样品中共得到908 120条有效序列,从不同土壤样品中,获得了4 623~5 394种细菌分类操作单元OTU,共涵盖了31门、92纲、130目、217科和363属的细菌。菌群分类发现,变形菌门(Protrobacteria )、绿弯菌门(Chloroflexi)和放线菌门(Actinobacteria)是主要的细菌类群,相对丰度占群落的55%~75%;不同作物根际土壤优势菌属也存在差异。与休闲地相比,种植作物可提高土壤细菌丰富度指数(Chao1和ACE指数)、香农指数(Shannon指数)和优势度指数(Si mpson指数),其中以藜麦处理最为明显,分别提高了19.59%、22.20%、7.84%和0.36%。通过相关性分析和多元分析,发现土壤细菌群落结构受土壤速效钾的影响较大。研究结果表明,通过种植不同的作物对土壤肥力状况的改变有较大影响,并且可以改变土壤细菌群落组成,研究结果为更深层次地了解土壤细菌群落与作物种类的关系提供理论依据。
英文摘要	Diversified crops are needed to optimize crop single production, which can increase biodiversity at yellow river pumping irrigation area in the central arid zone of Ningxia. In order to investigate the influence of different crops on the bacterial community structure and diversity of farmland soil, this study took millet (Setaria italica), black beans (Glycine max), quinoa (Chenopodium quinoa) and recreational soil as the objects, and measured the soil pH, total nitrogen, organic matter, available potassium, available phosphorus and alkali nitrogen content. Furthermore, the bacterial community structures were compared using Illumina MiSeq high-throughput sequencing technology. The results showed that the contents of soil organic matter, alkali nitrogen, available potassium and available phosphorus increased after planting quinoa, which were 2.88%, 19.12%, 112.04% and 39.90% higher than those in fallow land, respectively, but the pH value decreased, only 0.01 unit. A total of 908 120 effective sequences were obtained from the 5 groups of soil samples, and 4 623 to 5 394 bacterial OTU were obtained from different soil samples, covering 31 phyla, 92 classes, 130 orders, 217 families, 363 genera of bacteria. The flora classification found that Protrobacteria, Chloroflexi and Actinobacteria were the main bacterial groups with relative abundance accounting for 55%~75% of the community. The dominant genus in rhizosphere soil of different crops were also different. Compared with fallow land, planting crops could improve soil bacterial richness index, Shannon index and dominance index, among which quinoa treatment was the most obvious, increasing by 19.59%, 22.20%, 7.84%, 0.36%, respectively. Through multivariate analysis and correlation analysis, it was found that soil bacterial community structure was greatly affected by soil available potassium. The results showed that planting different crops had great influence on the change of soil fertility, and could change the composition of soil bacterial communities, providing theoretical support for a deeper understanding of the relationship between soil bacterial communities and crop species.
中文关键词	作物 ; 根际土壤 ; 细菌 ; 群落结构 ; 高通量测序技术
英文关键词	Crop Rhizosphere Bacteria Community structure High-throughput sequencing
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6870762
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353708
作者单位	孙倩, 宁夏大学农学院, 银川, 宁夏 750021, 中国. 吴宏亮, 宁夏大学农学院, 银川, 宁夏 750021, 中国. 康建宏, 宁夏大学农学院, 银川, 宁夏 750021, 中国. 陈阜, 中国农业大学农学院, 农业农村部农作制度重点实验室, 北京 100193, 中国.
推荐引用方式 GB/T 7714	孙倩,吴宏亮,陈阜,等. 基于高通量测序的几种不同作物根际土壤细菌群落结构和多样性分析[J],2020,28(8):1490-1498.
APA	孙倩,吴宏亮,陈阜,&康建宏.(2020).基于高通量测序的几种不同作物根际土壤细菌群落结构和多样性分析.农业生物技术学报,28(8),1490-1498.
MLA	孙倩,et al."基于高通量测序的几种不同作物根际土壤细菌群落结构和多样性分析".农业生物技术学报 28.8(2020):1490-1498.