干旱区生态环境知识资源中心(Arid): 干旱胁迫对C_3植物天山猪毛菜叶片C

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	干旱胁迫对C_3植物天山猪毛菜叶片C_4光合酶和delta~(13)C值的影响
其他题名	Changes in C_4 photosynthetic enzymes and delta~(13)C values of C_3 desert plant Salsola junatovii Botsch.under soil drought stress
	闻志彬; 夏春兰; 王玉兰
来源期刊	干旱区研究
ISSN	1001-4675
出版年	2020
卷号	37 期号:4
中文摘要	以藜科猪毛菜属具有代表性的C_3荒漠半灌木植物天山猪毛菜(Salsola junatovii Botsch.)为研究对象,采用盆栽控水试验设置正常水分与轻度、中度和重度3种胁迫处理,测定不同程度干旱胁迫下叶片含水量、4种C_4光合酶[磷酸烯醇式丙酮酸羧化酶(PEPC)、NADP-苹果酸酶(NADP-ME)、NAD-苹果酸酶(NAD-ME)和磷酸烯醇式丙酮酸羧激酶(PEPCK)]的活性以及蛋白表达量、稳定碳同位素比值(delta~(13)C值);同时测定6个野外不同年平均降水量地区叶片的delta~(13)C值,明确不同干旱胁迫对天山猪毛菜C_4光合酶和delta~(13)C值的影响;探讨天山猪毛菜是否会通过增强C_4途径的表达来适应干旱胁迫。结果显示:(1)植物叶片含水量随干旱胁迫的加剧而降低;(2)4种光合酶,仅有PEPC和NAD-ME受到干旱胁迫的影响。这两种酶的活性和蛋白表达量变化趋势基本一致,它们均随着干旱胁迫程度的加剧先增加,并在中度干旱胁迫达到最大值,然后在重度干旱胁迫下急剧下降。NADP-ME活性、PEPCK活性及其蛋白表达量均不受干旱胁迫的影响;(3)尽管在轻度和中度干旱胁迫下天山猪毛菜叶片delta~(13)C值升高,并在中度干旱胁迫下delta~(13)C值最高,但在胁迫处理下delta~(13)C值仍处于C_3植物范围内;野外样品叶片的delta~(13)C值表明,年降水量相对较少的3个地区样品delta ~(13)C值高。
英文摘要	Salsola junatovii Botsch.is a typical C_3 desert subshrub,belonging to the genus Salsola L.of the family Chenopodiaceae.It was used to investigate the changes of four C_4 photosynthetic enzymes,including phosphoenolpyruvate carboxylase(PEPC),NAD-malic enzyme(NAD-ME),NADP-malic enzyme(NADP-ME),and phosphoenolpyruvate carboxykinase(PEPCK),as well as delta~(13)C values in response to soil drought stress(including mild,moderate,and severe drought stress).A study of delta~(13)C values in samples from six different precipitation areas was performed to understand the transition of photosynthetic carbon assimilation pathway from C_3 to C_4 under soil drought stress.The results were as follows:(1)leaf water content decreased due to increasing drought stress,(2)in these four photosynthetic enzymes,only PEPC and NAD-ME activities were affected by drought stress.Changes in activities and relative protein levels of PEPC and NAD-ME were consistent.They increased by increasing drought stress and reached the maximum values under moderate drought stress.Then,they decreased sharply under severe drought stress conditions.The activity of NADP-ME,and the activity and relative protein level of PEPCK were not affected by drought stress,and(3)the delta~(13)C values increased under the mild and moderate drought stress and reached the maximum values under moderate drought stress conditions.The delta~(13)C values belonged to the range of C_3 plants under drought stress,and the delta~(13)C values of samples from the three areas with relatively low annual precipitation were higher.
中文关键词	光合碳同化途径 ; C_3荒漠植物 ; 干旱胁迫 ; C_4光合酶 ; 天山猪毛菜
英文关键词	photosynthetic carbon assimilation pathway C_3 desert plant soil drought stress C_4 photosynthetic enzymes Salsola junatovii
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Plant Sciences
CSCD记录号	CSCD:6834400
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353516
作者单位	闻志彬, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国. 夏春兰, 中国科学院新疆生态与地理研究所;;中国科学院大学, 中国科学院干旱区生物地理与生物资源重点实验室;;, 乌鲁木齐;;, ;;北京 830011;;101408. 王玉兰, 中国科学院新疆生态与地理研究所;;中国科学院大学, 中国科学院干旱区生物地理与生物资源重点实验室;;, 乌鲁木齐;;, ;;北京 830011;;101408.
推荐引用方式 GB/T 7714	闻志彬,夏春兰,王玉兰. 干旱胁迫对C_3植物天山猪毛菜叶片C_4光合酶和delta~(13)C值的影响[J]. 中国科学院新疆生态与地理研究所,2020,37(4).
APA	闻志彬,夏春兰,&王玉兰.(2020).干旱胁迫对C_3植物天山猪毛菜叶片C_4光合酶和delta~(13)C值的影响.干旱区研究,37(4).
MLA	闻志彬,et al."干旱胁迫对C_3植物天山猪毛菜叶片C_4光合酶和delta~(13)C值的影响".干旱区研究 37.4(2020).