干旱区生态环境知识资源中心(Arid): 耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响

Arid

	耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响
其他题名	Effects of drought-tolerance Rhizobium on the Dolichos lablab growth under drought stress
	张淑卿; 臧丹丹; 李剑峰; 郭金梅
来源期刊	干旱地区农业研究
ISSN	1000-7601
出版年	2020
卷号	38 期号:6
中文摘要	为探明耐旱根瘤菌对干旱胁迫下拉巴豆(Dolichos lablab L.)植株生长的影响,以拉巴豆耐旱根瘤菌Rhizobium radiobacter Y-1(Y-1)、Rhizobium radiobacter 4-4(4-4)和Rhizobium radiobacter 37-1(37-1)作为供试菌株,润高拉巴豆种子经表面灭菌并催芽后接种菌液(以等量无菌水为对照),以砂-石屑基质(塑料盆上部为石英砂,下部为石漠岩屑)进行沙培盆栽实验。通过控制无菌水添加量来模拟重度石漠化生境下的轻度、中度及重度干旱胁迫,并测定各处理幼苗生长和生理相关指标。结果表明:干旱胁迫下接种耐旱根瘤菌Y-1和37-1能显著促进拉巴豆幼苗的生长,处理30 d后,菌液处理幼苗的生长和生理指标均优于对照;重度干旱胁迫(30%田间持水量(theta_f))下,Y-1菌液处理的株高、叶面积、根长、根鲜重及根体积较对照分别增加50.25%、73.17%、72.41%,136.00%和289.47%(P<0.05),原初光能转化效率F_v/F_m和可溶性蛋白含量分别提升42.09%和108.57%,而叶片MDA含量仅为对照的60.66%(P<0.05);轻度至中度干旱胁迫(45%~ 60%theta_f)下,37-1菌液处理的幼苗根长为对照的39.93%~ 47.99%,而MDA含量仅为对照的72.14%~ 90.92%。拉巴豆种子接种耐旱根瘤菌Y-1和37-1能促进幼苗株高、叶面积、根鲜重和根体积的增加,提升叶片可溶性蛋白含量及叶片原初光能转化效率 ,减轻叶片损伤并提高根系活力,表明接种耐旱根瘤菌可促进拉巴豆在干旱胁迫下的生长,提升幼苗的抗旱能力。
英文摘要	To investigate the effects of drought-tolerant Rhizobium on the growth of Dolichos lablab seedlings under drought stress,a sand-culture experiment was conducted to evaluate the effect of Rhizobium radiobacter Y-1(Y-1),Rhizobium radiobacter 4-4 (4-4),and Rhizobium radiobacter 37-1 (37-1)on seedlings of Dolichos lablab under drought-stress condition.Surface sterilized and germinated seeds of Dolichos lablab cv.Rongai were sown in Plastics pots filled with clear sand at the top and rock debris (from rocky desertification area)at the bottom.The pots of each treatment were inoculated with sterilized water (control)or Rhizobium solution of Y-1,4-4,or 37-1.Calculated and supplied the sterilized water to simulate the mild,moderate,and severe drought stress in severe rocky desertification habitat.Results showed that the seedlings under drought stress were significantly promoted when seeds were inoculated with Rhizobium radiobacter Y-1 or Rhizobium radiobacter 37-1.On day 30,all of growth attributes and physiology of seedlings were significantly better than that of the control (inoculated with steri-lized water)under drought stress.Under severe drought stress (30% of field water capacity,theta_f),the plant height, leaf area,root length,root fresh weight and root volume of seedlings inoculated with Y-1 increased by 50.25%, 73.17%,72.41%,136.00%,and 289.47%,respectively,compared with those of the control.Plant soluble protein content increased by 108.57% and maximum quantum efficiency of PS Ⅱ photochemistry (F_v/F_m)increased by 42.09% compared with that of the control while leaf MDA content of Y-1 treatment was only 60.66% of the control (P<0.05).Under mild and moderate drought stress (45%~ 60% of theta_f),plant root length of 37-1 treatment was 39.93%~ 47.99% of that of the control while MDA content of 37-1 treatment was only 72.14% ~ 90.92% of that of the control.Inoculated with drought-tolerant Rhizobium radiobacter Y-1 and Rhizobium radiobacter 37-1 accelerated root growth,root volume,leaf area,and the formation of soluble protein,decreased leaf MDA content,and increased the root activity and the leaf maximum quantum efficiency of PSⅡ photochemistry under the drought stress,indicating that as a kind of cultivation method,the seed inoculated with drought-tolerant Rhizobium promoted the seedling growth and resistance under drought stress conditions.
中文关键词	耐旱根瘤菌 ; 促生作用 ; 拉巴豆 ; 干旱胁迫 ; 生理指标 ; 石漠退化生境
英文关键词	drought-tolerance Rhizobium promoting plant growth Dolichos lablab L. drought stress physiological index degraded rocky desert habitats
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6902094
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353454
作者单位	张淑卿, 贵州师范学院地理与资源学院;;贵州师范学院喀斯特生境土壤与环境生物修复研究所, ;;, 贵阳;;贵阳, 贵州;;贵州 550018;;550018, 中国. 郭金梅, 贵州师范学院地理与资源学院;;贵州师范学院喀斯特生境土壤与环境生物修复研究所, ;;, 贵阳;;贵阳, 贵州;;贵州 550018;;550018, 中国. 臧丹丹, 贵州师范学院地理与资源学院, 贵阳, 贵州 550018, 中国. 李剑峰, 贵州师范学院喀斯特生境土壤与环境生物修复研究所, 贵阳, 贵州 550018, 中国.
推荐引用方式 GB/T 7714	张淑卿,臧丹丹,李剑峰,等. 耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响[J],2020,38(6).
APA	张淑卿,臧丹丹,李剑峰,&郭金梅.(2020).耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响.干旱地区农业研究,38(6).
MLA	张淑卿,et al."耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响".干旱地区农业研究 38.6(2020).