Arid
沙米生长特性调查及遗传多样性分析
其他题名Investigation of growth characteristics and analysis of genetic diversity of Agriophyllum squarrosum
聂峰杰; 甘晓燕; 张丽; 巩檑; 刘璇; 杨文静; 宋玉霞
来源期刊干旱地区农业研究
ISSN1000-7601
出版年2020
卷号38期号:6页码:1-
中文摘要为明确不同原生地沙米的生长特性和遗传多样性,在宁夏、甘肃、内蒙古等不同原生地设置样方开展物候期调查、形态学观察、种子营养成分分析,利用SSR分子 标记对3个省、自治区的17个自然居群沙米进行遗传多样性分析,获得如下结果:不同原生地沙米的物候期、器官形态和种子营养成分含量有差异,其中甘肃民勤 地区的沙米株高达20~ 135 cm,种子千粒重达1.59 g,淀粉含量达307.46 mg·g~(-1)、蛋白质含量达103.36 mg·g~(-1)、粗脂肪含量达54.90 mg·g~(-1),均优于甘肃武威、宁夏银川市和内蒙古阿拉善地区的沙米;3个省、自治区的17份自然居群的沙米种质资源遗传多样性分析,检测到76个 条带,其中有39条多态性条带,多态性比率为51.3%;利用NTSYSpc (Version 2.10e)软件计算出17份沙米种质资源遗传相似性系数介于0.28~ 0.71之间。以0.58作为相似性系数分界点,将17份沙米资源聚为3类,其中宁夏滨河新区的资源聚为一类,内蒙古阿拉善地区9号资源和其他地区的15 份资源均来自腾格里沙漠腹地,各聚为一类。研究表明供试沙米资源的物候期、形态特征、种子营养成分及遗传多样性均与原生地的生态地理环境具有一定相关性。
英文摘要Agriophyllum squarrosum is a pioneer plant of mobile dune with strong ecological growth adaptability, rich nutrition in seeds and plants.It has many application values.In order to clarify the growth characteristics and genetic diversity of Agriophyllum squarrosum from different habitats,samples from Ningxia,Gansu,and Inner Mongolia were set up to carry out phenological investigation,morphological observation,and analysis of seed nutrition components.The genetic diversity of Agriophyllum squarrosum from 17 natural populations in 3 provinces/ autonomous regions was analyzed using SSR molecular markers.The results showed that there were differences in phenological organ morphology and seed nutrient content of Agriophyllum squarrosum from different habitats.The Agriophyllum squarrosum strains in Minqin,Gansu were as high as 20 ~ 135 cm,the seed weight was 1.59 g,the starch content was 307.46 mg·g~(-1),the protein content was 103.36 mg·g~(-1),and the crude fat content was 54.90 mg·g~(-1),all of which were better than those of the Agriophyllum squarrosum strains in Yinchuan of Ningxia,Alashan of Inner Mongolia,and Wuwei of Gansu.The genetic diversity analysis of Agriophyllum squarrosum germplasm resources from 17 natural populations in 3 provinces/autonomous regions detected 76 bands,39 of which were polymorphic, with a polymorphism rate of 51.3%.Using NTSYSpc (Version 2.10E)software,the genetic similarity coefficient of 17 Agriophyllum squarrosum germplasm resources in 3 provinces autonomous regions was calculated between 0.28 and 0.71,and 0.58 was taken as the cut-off point of similarity coefficient to cluster the 17 shami resources into 3 categories.The resources in Binhe New Area of Ningxia,No.9 resources in Alashan area of Inner Mongolia and 15 resources in other areas all came from the hinterland of the Tengger Desert,each of which was grouped into 1 category.The results showed that the phylogenetic characteristics of the tested Agriophyllum squarrosum were correlated with the ecological and geographical environment of the original habitat.
中文关键词沙米 ; 生长特性 ; 遗传多样性 ; SSR分析
英文关键词Agriophyllum squarrosum growth characteristics genetic diversity SSR analysis
类型Article
语种中文
收录类别CSCD
WOS类目Plant Sciences
CSCD记录号CSCD:6902089
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/353451
作者单位聂峰杰, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 甘晓燕, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 张丽, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 巩檑, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 刘璇, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 杨文静, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国. 宋玉霞, 宁夏农林科学院农业生物技术研究中心, 银川, 宁夏 750002, 中国.
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聂峰杰,甘晓燕,张丽,等. 沙米生长特性调查及遗传多样性分析[J],2020,38(6):1-.
APA 聂峰杰.,甘晓燕.,张丽.,巩檑.,刘璇.,...&宋玉霞.(2020).沙米生长特性调查及遗传多样性分析.干旱地区农业研究,38(6),1-.
MLA 聂峰杰,et al."沙米生长特性调查及遗传多样性分析".干旱地区农业研究 38.6(2020):1-.
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