干旱区生态环境知识资源中心(Arid): 苜蓿种植年限对土壤硝化潜势和氨氧化微生物丰度的影响

Arid

	苜蓿种植年限对土壤硝化潜势和氨氧化微生物丰度的影响
其他题名	Effects of years of alfalfa planting on nitrification potential and abundance of soil ammonia oxidation microorganisms
	张耀全; 马欣; 罗珠珠; 牛伊宁; 李玲玲; 蔡立群; 谢军红
来源期刊	干旱地区农业研究
ISSN	1000-7601
出版年	2020
卷号	38 期号:5
中文摘要	依托布设在黄土高原半干旱区的长期定位试验,通过高通量测序研究不同种植年限(L2003、L2005和L2012)苜蓿土壤氨氧化古菌(AOA)和氨氧化细菌(AOB)丰度,并分析揭示了影响氨氧化微生物丰度的环境因子。研究结果表明,土壤硝化潜势随苜蓿种植年限延长逐渐下降,其中,L2012、L20 05和L2003处理的硝化潜势分别为0.05、0.04、0.04 mug·g~(-1)·h~(-1),且处理间差异显著(P<0.05);土壤全氮含量随苜蓿种植年限延长逐渐增加,其中, L2012、L2005和L2003处理的全氮含量分别为1.02、1.14、1.18 g·kg~(-1),且处理间差异显著(P<0.05),L2003和L2005处理的全氮含量分别比L2012高15.69%和11.76%;土壤硝态氮含量表现为随种植年限延长显著增加(P<0.05), L2003处理和L2005处理硝态氮含量分别比L2012处理提高59.73%和33.62%。高通量测序发现,AOA amoA基因拷贝数为9.7510~6 ~ 12.6810~6个·g~(-1),明显高于AOB的5.0110~6 ~ 7.7010~6个·g~(-1),AOA丰度随苜蓿种植年限延长显著增加(P<0.05),但AOB丰度表现出随种植年限延长先升高后降低的趋势,这表明黄土高原半干旱区苜蓿土壤氨氧化微生物以AOA占主导地位。相关分析表明,AOA丰度与土壤全氮(r = 0.853)和硝态氮(r = 0.833)均呈极显著正相关,但与硝化潜势(r = -0.802)呈极显著负相关,AOB丰度与土壤氮素含量和硝化潜势没有明显相关性。由此可见,黄土高原半干旱区苜蓿土壤氨氧化微生物以AOA为优势类群 ,且对苜蓿种植年限有更强烈的响应,但其并未主导土壤硝化过程。
英文摘要	This experiment relied on the alfalfa (Medicago sativa) cultivation with different planting years (L2003,L2005,and L2012) on Loess Plateau. We investigated the soil nitrate N,ammonium N,nitrification potential, the abundance of ammonia oxidization Archaea(AOA) and ammonia oxidization Bacteria (AOB),and analyzed the effects of physicochemical indexes on the abundance of ammonia-oxidizing microorganisms. The results showed that the nitrification potential of soil gradually decreased with increasing years of alfalfa planting and different years of planting had significant differences (P < 0.05),which nitrification potentials of L2012,L2005 and L2003 were 0.05,0.04 mug·g~(-1)·h~(-1) and 0.04 mug·g~(-1)·h~(-1),respectively. Soil total N gradually significantly increased with increasing years of alfalfa planting (P<0.05),which total nitrogen contents of L2012,L2005 and L2003 were 1.02,1.14 g·kg~(-1) and 1.18 g·kg~(-1),respectively. The total N contents of L2003 and L2005 were higher than L2012 by 15.69% and 11.76%,respectively. Soil nitrate N significantly increased gradually with increasing years of alfalfa planting (P<0.05). The soil nitrate N contents of L2003 and L2005 were higher than that of L2012 by 59.73% and 33.62%,respectively. The number of copies of AOA amoA gene was 9.7510~6 ~12.68 10~6·g~(-1),which was significantly higher than 5.0110~6 ~7.7010~6·g~(-1) of AOB,and the AOA abundance gradually increased with increasing the years of alfalfa planting significantly. The AOB abundance showed a significant trend of increasing first and then decreasing with increasing years of alfalfa planting (P<0.01). This indicates that AOA was the dominant microorganism of ammonia oxidation in alfalfa soil in semi-arid area of loess plateau. The correlation analysis showed that AOA abundance was significantly positively correlated with total N (r = 0.853) and nitrate N (r = 0.833) but significantly negatively correlated with nitrification potential (r = -0.802),and AOB abundance was not significantly correlated with physicochemical properties. The results also showed that AOA was the dominant group of ammonia-oxidizing microorganisms in alfalfa soil in the semi-arid area of the Loess Plateau, which had a stronger response to alfalfa growth years,but it did not lead the soil nitrification process.
中文关键词	苜蓿 ; 种植年限 ; 氨氧化微生物丰度 ; 硝化潜势 ; 土壤氮素
英文关键词	alfalfa years of planting ammonia-oxidizing microorganisms abundance nitrification potential soil nitrogen
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6851198
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353446
作者单位	张耀全, 甘肃农业大学资源与环境学院, 兰州, 甘肃 730070, 中国. 马欣, 甘肃农业大学资源与环境学院, 兰州, 甘肃 730070, 中国. 罗珠珠, 甘肃农业大学资源与环境学院;;甘肃省干旱生境作物学省部共建国家重点实验室, ;;甘肃省干旱生境作物学省部共建国家重点实验室, 兰州;;兰州, 甘肃;;甘肃 730070;;730070, 中国. 蔡立群, 甘肃农业大学资源与环境学院;;甘肃省干旱生境作物学省部共建国家重点实验室, ;;甘肃省干旱生境作物学省部共建国家重点实验室, 兰州;;兰州, 甘肃;;甘肃 730070;;730070, 中国. 牛伊宁, 甘肃省干旱生境作物学省部共建国家重点实验室, 甘肃省干旱生境作物学省部共建国家重点实验室, 兰州, 甘肃 730070, 中国. 李玲玲, 甘肃省干旱生境作物学省部共建国家重点实验室, 甘肃省干旱生境作物学省部共建国家重点实验室, 兰州, 甘肃 730070, 中国. 谢军红, 甘肃省干旱生境作物学省部共建国家重点实验室, 甘肃省干旱生境作物学省部共建国家重点实验室, 兰州, 甘肃 730070, 中国.
推荐引用方式 GB/T 7714	张耀全,马欣,罗珠珠,等. 苜蓿种植年限对土壤硝化潜势和氨氧化微生物丰度的影响[J],2020,38(5).
APA	张耀全.,马欣.,罗珠珠.,牛伊宁.,李玲玲.,...&谢军红.(2020).苜蓿种植年限对土壤硝化潜势和氨氧化微生物丰度的影响.干旱地区农业研究,38(5).
MLA	张耀全,et al."苜蓿种植年限对土壤硝化潜势和氨氧化微生物丰度的影响".干旱地区农业研究 38.5(2020).