干旱区生态环境知识资源中心(Arid): 麻痹性贝类毒素的检测方法研究

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	麻痹性贝类毒素的检测方法研究
其他题名	Detection of paralytic shellfish poison in food samples
	郑仁锦; 林守二; 卢嘉丽; 杨艳; 黄宏南; 蔡茂荣
来源期刊	中国预防医学杂志
ISSN	1009-6639
出版年	2019
卷号	20 期号:12
中文摘要	目的建立超高效液相色谱-串联质谱法测定食物中毒样本中的麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的检测方法。方法用80%乙腈水(含0.1%的甲酸)超声振荡提取并离心,上清液经Oasis HLB固相萃取柱净化,用0.1%甲酸溶液洗脱。选用TSK-Gel Amide-80色谱柱(5mum,2.0mm* 150mm);梯度洗脱;电喷雾负离子源(electrospray ion source,ESI);多反应监测模式(multiple response monitoring,MRM);基质匹配外标法测定12种PSP浓度。结果dcGTX2,dcGTX3,GTX5,C1, C2,dcSTX均有检出,3份中毒患者吃剩食物的总毒力(STXeq)分别为9 295.6~19 001.4mug/kg,7份涉事海域养殖场及附近乡镇市售贝类中有5份检出PSP,STXeq范围5 091.7~23 292.8mug/kg,2份未检出。结论超高效液相色谱-串联质谱法操作简单易行,可准确、快速测定食物中毒样本中的PSP。
英文摘要	Objective To detect the paralytic shellfish poison(PSP)in food samples using ultra-performance liquid chromatography-tandem mass spectrometry method. Methods The PSPs in poisoned food samples were extracted by 80%acetonitrile solution(containing 0.1%formic acid)using super-sonic instrument.The supernatant was cleaned up with Oasis HLB (500mg/6ml)column and eluted with 0.1%formic acid solution. The target compounds were separated in TSK-Gel Amide-80column (5mum,2.0mm*150mm)by gradient elution,and detected by ultra-performance liquid chromatography-tandem mass spectrometry,identified by electrospray ion source(ESI)using multiple response monitoring (MRM),and quantified by external standards of sample matrix matching. Results dcGTX2,dcGTX3,GTX5,C1,C2,dcSTX were all detected,and the total virulence(STXeq)of leftover food from 3patients ranged from 9 295.6mug/kg to 19 001.4mug/kg. PAPs were detected from 5out of 7shellfish samples sold in involved mariculture farms and nearby townships with the total virulence(STXeq)ranging from 5 091.7mug/kg to 23 292.8mug/kg. Conclusions PAPs can be accurately and quickly detected in food samples by using ultra-performance liquid chromatography-tandem mass spectrometry method which is simple and easy to operate.
中文关键词	食物中毒 ; 超高效液相色谱-串联质谱法 ; 麻痹性贝类毒素 ; 双壳贝类 ; 固相萃取
英文关键词	Food poisoning Ultra-performance liquid chromatography-tandem mass spectrometry Paralytic shellfish poison Bivalves Solid phase extraction
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Public, Environmental & Occupational Health
CSCD记录号	CSCD:6698263
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353308
作者单位	郑仁锦, 福建省疾病预防控制中心, 福建省人兽共患病研究重点实验室, 福州, 福建 350001, 中国. 林守二, 福建省疾病预防控制中心, 福建省人兽共患病研究重点实验室, 福州, 福建 350001, 中国. 杨艳, 福建省疾病预防控制中心, 福建省人兽共患病研究重点实验室, 福州, 福建 350001, 中国. 黄宏南, 福建省疾病预防控制中心, 福建省人兽共患病研究重点实验室, 福州, 福建 350001, 中国. 卢嘉丽, 福建医科大学. 蔡茂荣, 漳州市疾病预防控制中心.
推荐引用方式 GB/T 7714	郑仁锦,林守二,卢嘉丽,等. 麻痹性贝类毒素的检测方法研究[J],2019,20(12).
APA	郑仁锦,林守二,卢嘉丽,杨艳,黄宏南,&蔡茂荣.(2019).麻痹性贝类毒素的检测方法研究.中国预防医学杂志,20(12).
MLA	郑仁锦,et al."麻痹性贝类毒素的检测方法研究".中国预防医学杂志 20.12(2019).