干旱区生态环境知识资源中心(Arid): HPLC-ESI-QTOF-MS法测定减肥保健食品中16种食欲抑制剂

Arid

	HPLC-ESI-QTOF-MS法测定减肥保健食品中16种食欲抑制剂
其他题名	Rapid determination of 16 kinds of illegally adulterated appetite inhibitors in weight controlling health food by HPLC-ESI-QTOF-MS
	孙婷婷; 徐彦红; 张雪梅; 郑绵冬
来源期刊	分析试验室
ISSN	1000-0720
出版年	2019
卷号	38 期号:12 页码:1449-1453
中文摘要	建立高效液相色谱-电喷雾离子化-四级杆串联飞行时间质谱法(HPLC-ESI-QTOFMS)高通量快速测定减肥保健食品中16种食欲抑制剂的含量。样品经甲醇超声萃取、Oasis MCX固相萃取柱净化,以甲醇和有乙酸铵溶液为流动相,梯度洗脱,采用QTOF-MS质谱仪进行分析。结果表明:16种食欲抑制剂在1.0 ~100.0 ng/mL范围内具有良好的线性关系,相关系数≥0.9995;方法检出限在0.03 ~0.30 mug/kg,方法定量限在0.10 ~1.00 mug/kg;加标回收率在91.7% ~99.2%,相对标准偏差在1.1% ~3.9%。
英文摘要	A method of high performance liquid chromatography-electrospray ionization-quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOF-MS) was established to determine the content of 16 kinds of illegally adulterated appetite inhibitors in weight controlling health food.The samples were ultrasonic extracted by methanol,purified by the solid phase extraction column of Oasis MCX,gradient eluted with methanol and ammonium acetate solution as the mobile phase,and analyzed by QTOF-MS mass spectrometer.The results showed that the calibration curves of 16 kinds of appetite inhibitors were linear in the range of 1.0 -100.0 ng/mL,and the correlation coefficients were greater than 0.9995.The limits of quantitation were in the range of 0.03 -0.30 mug/kg,and the limits of detection were in the range of 0.10 -1.00 mug/kg.The average recoveries were in the range of 91.7%- 99.2%,and the relative standard deviations were in the range of 1.1% -3.9%.
中文关键词	减肥保健食品 ; 食欲抑制剂 ; 非法添加
英文关键词	HPLC-ESI-QTOF-MS Weight controlling health food HPLC-ESI-QTOF-MS Appetite inhibitor Illegally adulterated
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Food Science & Technology
CSCD记录号	CSCD:6641218
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/353221
作者单位	孙婷婷, 徐州工业职业技术学院, 徐州, 江苏 221140, 中国. 徐彦红, 徐州工业职业技术学院, 徐州, 江苏 221140, 中国. 张雪梅, 徐州工业职业技术学院, 徐州, 江苏 221140, 中国. 郑绵冬, 徐州工业职业技术学院, 徐州, 江苏 221140, 中国.
推荐引用方式 GB/T 7714	孙婷婷,徐彦红,张雪梅,等. HPLC-ESI-QTOF-MS法测定减肥保健食品中16种食欲抑制剂[J],2019,38(12):1449-1453.
APA	孙婷婷,徐彦红,张雪梅,&郑绵冬.(2019).HPLC-ESI-QTOF-MS法测定减肥保健食品中16种食欲抑制剂.分析试验室,38(12),1449-1453.
MLA	孙婷婷,et al."HPLC-ESI-QTOF-MS法测定减肥保健食品中16种食欲抑制剂".分析试验室 38.12(2019):1449-1453.