干旱区生态环境知识资源中心(Arid): G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

Arid

DOI	10.14202/vetworld.2021.104-112
	G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep
	Abdelgadir, Amani Z.; Musa, Lutfi M. A.; Jawasreh, Khaleel, I; Saleem, Aubai O.; El-Hag, Faisal; Ahmed, Mohamed-Khair A.
通讯作者	Jawasreh, KI (corresponding author), Jordan Univ Sci & Technol, Dept Anim Prod, Fac Agr, Irbid, Jordan.
来源期刊	VETERINARY WORLD
ISSN	0972-8988
EISSN	2231-0916
出版年	2021
卷号	14 期号:1 页码:104-112
英文摘要	Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p <= 0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.
英文关键词	common ancestor growth differentiation factor 9 gene similarity and identity Sudanese sheep
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000613904100002
WOS关键词	CLOSTRIDIUM-PERFRINGENS ; OVULATION RATE ; GDF9 GENE ; POLYMORPHISM ; PROLIFICACY ; IDENTIFICATION ; ASSOCIATION ; HOLSTEIN ; PROTEIN ; BMP15
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352986
作者单位	[Abdelgadir, Amani Z.] Omdurman Islamic Univ, Fac Agr, Dept Anim Prod, Omdurman, Sudan; [Musa, Lutfi M. A.; Ahmed, Mohamed-Khair A.] Univ Khartoum, Fac Anim Prod, Dept Anim Breeding & Genet, Khartoum, Sudan; [Musa, Lutfi M. A.] League Arab States, Arab Ctr Studies Arid Zones & Dry Land, Damascus, Syria; [Jawasreh, Khaleel, I] Jordan Univ Sci & Technol, Dept Anim Prod, Fac Agr, Irbid, Jordan; [Saleem, Aubai O.] Africa City Technol, Dept Bioinformat, Khartoum, Sudan; [El-Hag, Faisal] Tottori Univ, Arid Land Res Ctr ALRC, 1390 Hamasaka, Tottori 6800001, Japan
推荐引用方式 GB/T 7714	Abdelgadir, Amani Z.,Musa, Lutfi M. A.,Jawasreh, Khaleel, I,et al. G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep[J],2021,14(1):104-112.
APA	Abdelgadir, Amani Z.,Musa, Lutfi M. A.,Jawasreh, Khaleel, I,Saleem, Aubai O.,El-Hag, Faisal,&Ahmed, Mohamed-Khair A..(2021).G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep.VETERINARY WORLD,14(1),104-112.
MLA	Abdelgadir, Amani Z.,et al."G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep".VETERINARY WORLD 14.1(2021):104-112.