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	Quantitative Expression Analysis through Transcript Profiling for Drought Stress in Cicer Arietinum L.
	Juhi, Bhardwaj; Nilima, Kumari; Nehal, Mittal; Renul, Yadav; Kumar, Singh Rajesh; Rajendra, Kumar
通讯作者	Rajendra, K (corresponding author), Sardar Vallabhbhai Patel Univ Agr & Technol, Dept Biotechnol, Meerut 250110, Uttar Pradesh, India. ; Rajendra, K (corresponding author), Indian Agr Res Inst, Div Genet, New Delhi 110012, India.
来源期刊	RESEARCH JOURNAL OF BIOTECHNOLOGY
ISSN	2278-4535
出版年	2021
卷号	16 期号:3 页码:26-32
英文摘要	Chickpea, one amongst the major pulse crops, is grown mainly in the arid and semi-arid regions. Drought is one of the very important abiotic stresses that changes plant water status and limits plant growth and development. Expression profiles of genes involved in conferring stress tolerance could be utilized to get improved tolerant varieties. In present study, chickpea accessions having diverse levels of drought-tolerance (SBD 377 and PUSA 1103) were quantitatively analysed for variations in transcript profiles during drought stress. 8 gene sequences from drought stressed EST library were selected for gene expression studies. The sequences were subjected to homology search through a BLAST. Out of which 5 genes were found to be up regulated and 3 genes were down regulated with respect to expression of housekeeping genes Ubiquitin and Actin. The increase and decrease in gene expression varied from 0.22 % to 14.82 % fold. The maximum gene expression was expressed by DREB gene with the minimum gene expression by aquaporin like water channel protein with the relative fold decrease of 0.22 % during drought stress whereas the proline rich protein expressed 0.28 % relative fold decrease in gene expression. The findings have confirmed involvement of PUSA 1103 derived DREB gene, hypoxia conserved region protein gene, sulferodoxin protein gene, glutathione peroxidase phgpx gene and serine type protein genes in conferring drought stress to the plant. These gene(s) should be utilized for incorporation of drought tolerance in chickpea and other crop improvement programmes.
英文关键词	EST Drought Gene Expression Transcriptome Proline rich protein
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000624442900004
WOS关键词	GENE-EXPRESSION ; COLD ; ARABIDOPSIS ; CHICKPEA
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352954
作者单位	[Juhi, Bhardwaj; Nehal, Mittal; Renul, Yadav; Kumar, Singh Rajesh; Rajendra, Kumar] Sardar Vallabhbhai Patel Univ Agr & Technol, Dept Biotechnol, Meerut 250110, Uttar Pradesh, India; [Juhi, Bhardwaj; Nilima, Kumari] Banasthali Vidyapeeth, Dept Biosci & Biotechnol, Vanasthali 304022, Rajasthan, India; [Renul, Yadav] Amity Univ, AIOA, Noida 201303, Uttar Pradesh, India; [Kumar, Singh Rajesh; Rajendra, Kumar] Indian Agr Res Inst, Div Genet, New Delhi 110012, India
推荐引用方式 GB/T 7714	Juhi, Bhardwaj,Nilima, Kumari,Nehal, Mittal,et al. Quantitative Expression Analysis through Transcript Profiling for Drought Stress in Cicer Arietinum L.[J],2021,16(3):26-32.
APA	Juhi, Bhardwaj,Nilima, Kumari,Nehal, Mittal,Renul, Yadav,Kumar, Singh Rajesh,&Rajendra, Kumar.(2021).Quantitative Expression Analysis through Transcript Profiling for Drought Stress in Cicer Arietinum L..RESEARCH JOURNAL OF BIOTECHNOLOGY,16(3),26-32.
MLA	Juhi, Bhardwaj,et al."Quantitative Expression Analysis through Transcript Profiling for Drought Stress in Cicer Arietinum L.".RESEARCH JOURNAL OF BIOTECHNOLOGY 16.3(2021):26-32.