干旱区生态环境知识资源中心(Arid): From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes

Arid

DOI	10.1007/s42995-020-00064-w
	From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes
	Salam, Nimaichand; Xian, Wen-Dong; Asem, Mipeshwaree Devi; Xiao, Min; Li, Wen-Jun
通讯作者	Li, WJ (corresponding author), Sun Yat Sen Univ, Guangdong Prov Key Lab Plant Resources, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Peoples R China. ; Li, WJ (corresponding author), Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Life Sci, Guangzhou 510275, Peoples R China. ; Li, WJ (corresponding author), Sun Yat Sen Univ, Sch Ecol, Guangzhou 510275, Peoples R China. ; Li, WJ (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China.
来源期刊	MARINE LIFE SCIENCE & TECHNOLOGY
ISSN	2096-6490
EISSN	2662-1746
出版年	2021
卷号	3 期号:2 页码:132-147
英文摘要	Earth is dominated by a myriad of microbial communities, but the majority fails to grow under in situ laboratory conditions. The basic cause of unculturability is that bacteria dominantly occur as biofilms in natural environments. Earlier improvements in the culture techniques are mostly done by optimizing media components. However, with technological advancement particularly in the field of genome sequencing and cell imagining techniques, new tools have become available to understand the ecophysiology of microbial communities. Hence, it becomes easier to mimic environmental conditions in the culture plate. Other methods include co-culturing, emendation of growth factors, and cultivation after physical cell sorting. Most recently, techniques have been proposed for bacterial cultivation by employing genomic data to understand either microbial interactions (network-directed targeted bacterial isolation) or ecosystem engineering (reverse genomics). Hopefully, these techniques may be applied to almost all environmental samples, and help fill the gaps between the cultured and uncultured microbial communities.
英文关键词	Biofilms Ecophysiology Unculturability Co-occurrence network Reverse genomics
类型	Review
语种	英语
开放获取类型	Bronze
收录类别	ESCI
WOS记录号	WOS:000649455400003
WOS关键词	MODERATELY HALOPHILIC BACTERIUM ; HALOTOLERANS SP NOV. ; SALT-LAKE SEDIMENT ; DESERTI SP NOV. ; ALBA GEN. NOV. ; XINJIANGENSIS SP-NOV. ; QUINGHAIENSIS SP NOV. ; SALSUGINIS SP NOV. ; SALIPHILUS SP NOV. ; SEDIMINIS SP NOV.
WOS类目	Marine & Freshwater Biology
WOS研究方向	Marine & Freshwater Biology
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352915
作者单位	[Salam, Nimaichand; Xian, Wen-Dong; Asem, Mipeshwaree Devi; Xiao, Min; Li, Wen-Jun] Sun Yat Sen Univ, Guangdong Prov Key Lab Plant Resources, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Peoples R China; [Salam, Nimaichand; Xian, Wen-Dong; Asem, Mipeshwaree Devi; Xiao, Min; Li, Wen-Jun] Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Life Sci, Guangzhou 510275, Peoples R China; [Salam, Nimaichand; Xian, Wen-Dong; Asem, Mipeshwaree Devi; Xiao, Min; Li, Wen-Jun] Sun Yat Sen Univ, Sch Ecol, Guangzhou 510275, Peoples R China; [Li, Wen-Jun] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China
推荐引用方式 GB/T 7714	Salam, Nimaichand,Xian, Wen-Dong,Asem, Mipeshwaree Devi,et al. From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes[J]. 中国科学院新疆生态与地理研究所,2021,3(2):132-147.
APA	Salam, Nimaichand,Xian, Wen-Dong,Asem, Mipeshwaree Devi,Xiao, Min,&Li, Wen-Jun.(2021).From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes.MARINE LIFE SCIENCE & TECHNOLOGY,3(2),132-147.
MLA	Salam, Nimaichand,et al."From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes".MARINE LIFE SCIENCE & TECHNOLOGY 3.2(2021):132-147.