干旱区生态环境知识资源中心(Arid): Phenetic Characterization of Nitrogen Fixing Azotobacter from Rhizospheric Soil of Southern Rajasthan

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DOI	10.22207/JPAM.15.1.40
	Phenetic Characterization of Nitrogen Fixing Azotobacter from Rhizospheric Soil of Southern Rajasthan
	Jain, Devendra; Kaur, Gunnjeet; Bhojiya, Ali Asger; Chauhan, Surya; Khandelwal, S. K.; Meena, R. H.; Rajpurohit, Deepak; Mohanty, Santosh Ranjan
通讯作者	Jain, D (corresponding author), Maharana Pratap Univ Agr & Technol, Dept Mol Biol & Biotechnol, Rajasthan Coll Agr, Udaipur 313001, Rajasthan, India.
来源期刊	JOURNAL OF PURE AND APPLIED MICROBIOLOGY
ISSN	0973-7510
EISSN	2581-690X
出版年	2021
卷号	15 期号:1 页码:428-436
英文摘要	The present research was conducted to characterize the indigenous plant growth promoting (PGP) Azotobacter strains isolated from plant root interface of semi-arid regions of Rajasthan (India) and to study their potential to be used as bio-fertilizers. A total of 172 Azotobacter strains were isolated, purified and based on the morphological test i.e. gram staining, pigmentation, cyst formation, fluorescence etc, broadly classified as Azotobacter. Further the secluded strains were examined for biochemical analysis and plant growth promoting characters. All the isolates showed different biochemical characteristics and significant PGP traits. IAA activity of the Azotobacter strains ranges from 54.5-6000 mu g/mL. Ammonia, HCN and siderophore was produced by 92.4%, 78.4% and 80.23% of the total isolates respectively. Solubilization of phosphate was observed in 97.6% of the total isolates. These strains were also characterized for qualitative and quantitative N-2 fixation abilities and the result indicated that 114 strains showed positive results on nitrogen free malate agar medium (NFMM) containing bromothymol blue (BTB) and able to produce 18.93-475.6 N-moles C2H4 mg protein(-1)h(-1) of acetylene reduced by Azotobacter strains. In vitro pot studies revealed that the selected native Azotobacter strains having high ARA results significantly increase the plant growth characters. Shoot length, root length, root number and chlorophyll content and leaf number increases by 45.62%, 17.60%, 97.49%, 49.69% and 27.83% respectively in pot inoculated with AZO23-3 as compared to control. These effective strains can further be utilized for development of effective microbial formulations.
英文关键词	Biochemical Test Cyst Pigmentation Antibiotic sensitivity Acetylene Reductase Assay Pot study
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000632041100040
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352903
作者单位	[Jain, Devendra; Kaur, Gunnjeet; Bhojiya, Ali Asger; Chauhan, Surya; Khandelwal, S. K.; Rajpurohit, Deepak] Maharana Pratap Univ Agr & Technol, Dept Mol Biol & Biotechnol, Rajasthan Coll Agr, Udaipur 313001, Rajasthan, India; [Kaur, Gunnjeet] Dr KN Modi Univ, Sch Agr Sci, Tonk 304021, Rajasthan, India; [Bhojiya, Ali Asger] Mewar Univ, Dept Agr & Vet Sci, Chittaurgarh 312901, Rajasthan, India; [Meena, R. H.] MPUAT, Dept Soil Sci & Agr Chem, Rajasthan Coll Agr, Udaipur 313001, Rajasthan, India; [Mohanty, Santosh Ranjan] Indian Council Agr Res, Indian Inst Soil Sci, Bhopal 462038, Madhya Pradesh, India
推荐引用方式 GB/T 7714	Jain, Devendra,Kaur, Gunnjeet,Bhojiya, Ali Asger,et al. Phenetic Characterization of Nitrogen Fixing Azotobacter from Rhizospheric Soil of Southern Rajasthan[J],2021,15(1):428-436.
APA	Jain, Devendra.,Kaur, Gunnjeet.,Bhojiya, Ali Asger.,Chauhan, Surya.,Khandelwal, S. K..,...&Mohanty, Santosh Ranjan.(2021).Phenetic Characterization of Nitrogen Fixing Azotobacter from Rhizospheric Soil of Southern Rajasthan.JOURNAL OF PURE AND APPLIED MICROBIOLOGY,15(1),428-436.
MLA	Jain, Devendra,et al."Phenetic Characterization of Nitrogen Fixing Azotobacter from Rhizospheric Soil of Southern Rajasthan".JOURNAL OF PURE AND APPLIED MICROBIOLOGY 15.1(2021):428-436.