干旱区生态环境知识资源中心(Arid): Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham

Arid

DOI	10.9734/JPRI/2021/v33i27A31494
	Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham
	Younus, Muhammad; ul Hasan, Muhammad Mohtasheem; Ijaz, Shakeel; Riaz, Razia; Ahmad, Khalil; Hanif, Muhammad; Khan, Mohsin Abbas; Abdullah, Muhammad; Shah, Muhammad Kamal; Nisar, Uzair
通讯作者	Younus, M (corresponding author), Islamia Univ Bahawalpur, Fac Pharm, Dept Pharmacognosy, Bahawalpur, Pakistan. ; Younus, M (corresponding author), Univ Karachi, Fac Pharm, Dept Pharmacognosy, Karachi, Pakistan.
来源期刊	JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL
ISSN	2456-9119
出版年	2021
卷号	33 期号:27A 页码:58-67
英文摘要	Euphorbia nivulia (EN) one of the members of Euphorbiaceae family, is a medicinal plant of Cholistan Desert (Punjab, Pakistan) that is traditionally used for a number of diseases. The plant is enriched with many phyto-constituents including flavonoids, triterpenes and polyphenols. In present study, crude extract as well as various fractions were assessed for phytochemical analysis, HPLC profiling, radical scavenging property and total phenolic and flavonoid contents. Hydro-alcoholic (70%) crude extract of EN was subjected to fractionation using different solvents including n-hexane, chloroform, butanol and aqueous. After performing the phytochemical screening and HPLC profiling, antioxidant activity was estimated by using DPPH and FRAP assays. Total flavonoid and phenolic contents were also estimated. HPLC analysis confirmed the presence of flavonoids and polyphenols like quercitin, gallic acid, caffeic acid, vanillic acid, benzoic acid, chlorogenic acid, syringic acid and ferulic acid in crude extract as well as butanol and aqueous fractions. Results revealed that butanol fraction showed maximum phenolic (143.26 +/- 2.65 mg/g GA/g) and crude extract showed maximum flavonoid (69.80 +/- 1.212 mg/g Q) contents. Maximum antioxidant potential was displaced by butanol fraction which was IC50=0.04 +/- 0.02 by DPPH and 1193.77 +/- 12.4 mu mol TE/ml by FRAP respectively. Current study is the first information about the HPLC profiling of phenolic compounds in EN and its antioxidant potential that could be a step forward towards ethno-pharmacological based phyto-medicine.
英文关键词	Euphorbia nivulia antioxidant polyphenols flavonoids HPLC
类型	Article
语种	英语
开放获取类型	gold
收录类别	ESCI
WOS记录号	WOS:000669973500009
WOS关键词	IN-VITRO ; FLAVONOIDS ; EXTRACTION ; PHENOLICS ; CAPACITY ; SOLVENT ; FRUITS ; ORGANS ; PLANTS ; FOOD
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352900
作者单位	[Younus, Muhammad] Islamia Univ Bahawalpur, Fac Pharm, Dept Pharmacognosy, Bahawalpur, Pakistan; [Younus, Muhammad; ul Hasan, Muhammad Mohtasheem] Univ Karachi, Fac Pharm, Dept Pharmacognosy, Karachi, Pakistan; [Ijaz, Shakeel] Quaid E Azam Coll Pharm, Pakpatan Rd, Sahiwal, Pakistan; [Riaz, Razia] Univ Agr Faisalabad, Hitech Lab, Faisalabad, Pakistan; [Ahmad, Khalil] Islamia Univ Bahawalpur, Univ Coll Convent Med, Bahawalpur, Pakistan; [Hanif, Muhammad] Benazir Bhutto Shaheed Univ, Dept Pharm, Karachi, Pakistan; [Khan, Mohsin Abbas] Islamia Univ Bahawalpur, Fac Pharm, Dept Pharmaceut Chem, Bahawalpur, Pakistan; [Abdullah, Muhammad] Islamia Univ Bahawalpur, Cholistan Inst Desert Studies, Bahawalpur, Pakistan; [Shah, Muhammad Kamal] Gomal Univ, Fac Vet Sci, Dera Ismail Khan, Pakistan; [Nisar, Uzair] Zia Ud Din Univ, Fac Pharm, Karachi, Pakistan
推荐引用方式 GB/T 7714	Younus, Muhammad,ul Hasan, Muhammad Mohtasheem,Ijaz, Shakeel,et al. Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham[J],2021,33(27A):58-67.
APA	Younus, Muhammad.,ul Hasan, Muhammad Mohtasheem.,Ijaz, Shakeel.,Riaz, Razia.,Ahmad, Khalil.,...&Nisar, Uzair.(2021).Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham.JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL,33(27A),58-67.
MLA	Younus, Muhammad,et al."Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham".JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL 33.27A(2021):58-67.