干旱区生态环境知识资源中心(Arid): GENETIC DIVERSITY AND NETWORK WITHIN DESSERT BANANAS (MUSA ACUMINATA CV. AA AND AAA) INFERRED BY NEWLY DESIGNED MATK MARKER

Arid

	GENETIC DIVERSITY AND NETWORK WITHIN DESSERT BANANAS (MUSA ACUMINATA CV. AA AND AAA) INFERRED BY NEWLY DESIGNED MATK MARKER
	Hariyanto, Sucipto; Ainiyah, Rojaunnajah Kartika; Utami, Edy Setiti Wida; Hapsari, Lia
通讯作者	Hapsari, L (corresponding author), Indonesian Inst Sci, Purwodadi Bot Garden, Res Ctr Plant Conservat & Bot Gardens, St Surabaya Malang Km 65, Pasuruan 67163, East Java, Indonesia.
来源期刊	INTERNATIONAL JOURNAL OF CONSERVATION SCIENCE
ISSN	2067-533X
EISSN	2067-8223
出版年	2021
卷号	12 期号:2 页码:585-598
英文摘要	This study aims to evaluate the genetic diversity and network within ten local cultivars of dessert bananas (Musa acuminata cv. AA and AAA) from Indonesia using newly designed matK marker. Result showed that PCR amplifications were successfully carried out. Total aligned and selected sequences were 729 bp, with nucleotide composition high in AT (67.60%) than GC (32.40%). Sequences variation shows high conservation level, comprising 97.94% monomorphics, 0.82% polymorphics, 0.96% InDels, and 0.27% missing data; with high genetic similarity 99.44%-100%. Haplotype diversity was high (Hd=0.844 +/- 0.103), forming six haplotypes but low in nucleotide diversity (pi=0.231 +/- 0.053). Both haplotype network and phylogenetic tree were separated into two clades, following its genomic subgrouping. Clade I comprised of Inarnibal (AA), Cavendish (AAA) and Gros Michel (AAA). Clade II comprised of Sucrier (AA) and Red (AAA). The genetic diversity within genomic group AAA is higher than AA. Both genomic groups cannot be clearly separated due to continuous genetic variation and mutations. Six point mutations were identified; mostly due to substitution of transversion than transition (Ti/Tv=0.5). The finding of this study suggests that the newly designed matK marker is effective for PCR and sufficient to discriminate at intraspecific level of desert bananas.
英文关键词	Genetic conservation Haplotype Molecular barcode Phylogenetic Polymorhism
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000661881100020
WOS关键词	PHYLOGENETIC ANALYSIS ; CLIMATE-CHANGE ; EAST JAVA ; ZINGIBERACEAE ; RBCL ; SPP.
WOS类目	Art
WOS研究方向	Art
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352850
作者单位	[Hariyanto, Sucipto; Ainiyah, Rojaunnajah Kartika; Utami, Edy Setiti Wida] Airlangga Univ, Fac Sci & Technol, Dept Biol, St Dr Ir H Soekarno, Surabaya 60115, East Java, Indonesia; [Hapsari, Lia] Indonesian Inst Sci, Purwodadi Bot Garden, Res Ctr Plant Conservat & Bot Gardens, St Surabaya Malang Km 65, Pasuruan 67163, East Java, Indonesia
推荐引用方式 GB/T 7714	Hariyanto, Sucipto,Ainiyah, Rojaunnajah Kartika,Utami, Edy Setiti Wida,et al. GENETIC DIVERSITY AND NETWORK WITHIN DESSERT BANANAS (MUSA ACUMINATA CV. AA AND AAA) INFERRED BY NEWLY DESIGNED MATK MARKER[J],2021,12(2):585-598.
APA	Hariyanto, Sucipto,Ainiyah, Rojaunnajah Kartika,Utami, Edy Setiti Wida,&Hapsari, Lia.(2021).GENETIC DIVERSITY AND NETWORK WITHIN DESSERT BANANAS (MUSA ACUMINATA CV. AA AND AAA) INFERRED BY NEWLY DESIGNED MATK MARKER.INTERNATIONAL JOURNAL OF CONSERVATION SCIENCE,12(2),585-598.
MLA	Hariyanto, Sucipto,et al."GENETIC DIVERSITY AND NETWORK WITHIN DESSERT BANANAS (MUSA ACUMINATA CV. AA AND AAA) INFERRED BY NEWLY DESIGNED MATK MARKER".INTERNATIONAL JOURNAL OF CONSERVATION SCIENCE 12.2(2021):585-598.