干旱区生态环境知识资源中心(Arid): Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya

Arid

DOI	10.1016/j.heliyon.2021.e06867
	Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya
	Muindi, Mercy Martha; Muthini, Morris; Njeru, Ezekiel Mugendi; Maingi, John
通讯作者	Njeru, EM (corresponding author), Kenyatta Univ, Dept Biochem Microbiol & Biotechnol, POB 43844-00100, Nairobi, Kenya.
来源期刊	HELIYON
EISSN	2405-8440
出版年	2021
卷号	7 期号:4
英文摘要	Cowpea (Vigna unguiculata (L.) Walp) is an important multipurpose legume crop grown in arid and semi-arid areas of sub-Saharan Africa. The crop associates with a wide diversity of high ecological value rhizobia bacteria, improving biological soil fertility and crop production. Here, we evaluated the symbiotic efficiency (SE) and genetic diversity of native rhizobia isolated from root nodules of cowpea genotypes cultivated in semi-arid areas of lower Eastern Kenya. Rhizobia trapping and SE experiments were done in the greenhouse while genetic diversity was evaluated based on 16S rRNA gene sequencing. Twenty morphologically distinct isolates representing a total of 94 isolates were used for genetic analysis. After 16S rRNA gene sequencing, the isolates closely resembled bacteria belonging to the genus Rhizobium, Paraburkholderia and non-rhizobial endophytes (Enterobacter, Strenotrophomonas and Pseudomonas). This study also reports for the first time the presence of an efficient native cowpea nodulating Beta-Rhizobia (Paraburkholderia phenoliruptrix BR3459a) in Africa. Symbiotic efficiency of the native rhizobia isolates varied (p < 0.0001) significantly. Remarkably, two isolates, M2 and M3 recorded higher SE of 82.49 % and 72.76 % respectively compared to the commercial strain Bradyrhizobium sp. USDA 3456 (67.68 %). Our results form an important step in the development of efficient microbial inoculum and sustainable food production.
英文关键词	Cowpea Genetic diversity Rhizobia Symbiotic efficiency Kenya
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000646310700007
WOS关键词	PLANT-GROWTH ; INOCULATION ; DIVERSITY ; STRAINS ; YIELD ; SOILS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352837
作者单位	[Muindi, Mercy Martha; Muthini, Morris; Njeru, Ezekiel Mugendi; Maingi, John] Kenyatta Univ, Dept Biochem Microbiol & Biotechnol, POB 43844-00100, Nairobi, Kenya
推荐引用方式 GB/T 7714	Muindi, Mercy Martha,Muthini, Morris,Njeru, Ezekiel Mugendi,et al. Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya[J],2021,7(4).
APA	Muindi, Mercy Martha,Muthini, Morris,Njeru, Ezekiel Mugendi,&Maingi, John.(2021).Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya.HELIYON,7(4).
MLA	Muindi, Mercy Martha,et al."Symbiotic efficiency and genetic characterization of rhizobia and non rhizobial endophytes associated with cowpea grown in semi-arid tropics of Kenya".HELIYON 7.4(2021).