干旱区生态环境知识资源中心(Arid): Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region

Arid

DOI	10.4238/gmr18778
	Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region
	Santos, V. N.; Gama, R. N. C. S.; Santos, C. A. F.
通讯作者	Santos, CAF (corresponding author), Empresa Brasileira Pesquisa Agr Embrapa Semiarido, Petrolina, PE, Brazil.
来源期刊	GENETICS AND MOLECULAR RESEARCH
EISSN	1676-5680
出版年	2021
卷号	20 期号:2
英文摘要	The umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars.
英文关键词	Umbu tree SSR Plant breeding SSR
类型	Article
语种	英语
开放获取类型	Bronze
收录类别	ESCI
WOS记录号	WOS:000671202600001
WOS关键词	MARKERS
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352817
作者单位	[Santos, V. N.; Gama, R. N. C. S.] Univ Estadual Feira de Santana, Programa Recursos Genet Vegetais, Feira De Santana, BA, Brazil; [Santos, C. A. F.] Empresa Brasileira Pesquisa Agr Embrapa Semiarido, Petrolina, PE, Brazil
推荐引用方式 GB/T 7714	Santos, V. N.,Gama, R. N. C. S.,Santos, C. A. F.. Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region[J],2021,20(2).
APA	Santos, V. N.,Gama, R. N. C. S.,&Santos, C. A. F..(2021).Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region.GENETICS AND MOLECULAR RESEARCH,20(2).
MLA	Santos, V. N.,et al."Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region".GENETICS AND MOLECULAR RESEARCH 20.2(2021).