干旱区生态环境知识资源中心(Arid): Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel (Camelus dromedarius L.)

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DOI	10.1016/j.dib.2020.106663
	Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel (Camelus dromedarius L.)
	Banerjee, Somesh; Singh, Ajit; Rawat, Jagveer; Bansal, Nitish; Maan, Sushila
通讯作者	Singh, A (corresponding author), Dept Vet Microbiol, Immunol Sect, Hisar, Haryana, India. ; Singh, A (corresponding author), Govt West Bengal, Dept ARD, Chakulia, Kolkata, Uttar Dinajpur, India.
来源期刊	DATA IN BRIEF
ISSN	2352-3409
出版年	2021
卷号	34
英文摘要	Next-generation sequences (NGS) dataset of nanobody (Nb) clones in a phage display library (PDL) is of immense value as it serves in many different ways, such as: i). estimating the library size, ii). improving selection and identification of Nbs, iii). informing about frequency of V gene families, diversity and length of CDRs, iv). high resolution analysis of natural and synthetic libraries, etc. [1-3]. We used a fraction of our previously constructed PDL of Nbs derived from an E. coli lipopolysaccharide-immunized Indian desert camel in order to obtain the dataset of NGS reads of Nbs. The cryo-preserved transformants library was revived to extract the Nb-encoding VHH (inserts)-pHEN4 (vector) DNA pool. The DNA sample was used for amplifying VHH pool by PCR [6]. The VHH amplicons band was gel-purified and subjected to NGS using Illumina MiSeqTM platform. 'Nextra XT micro V2 Index' kit was used for the Nb library DNA sample sequencing, with the adaptors: 'i7' (N706: TAGGCATG) and 'i5' (S517: GCGTAAGA). The raw data comprised of a total read count of 182146 (matched= 179591; unmatched=2555), with average read length of 130.33 bases and a total of 23.74 Mb. Of 179591 matched reads, 142004 were paired reads and 37587 broken paired reads. The raw data of NGS reads was submitted to NCBI Sequence Reads Archive accessible at URL: https://www.ncbi.nlm.nih.gov/Traces/study/ ?acc=PRJNA516512 (dataset ref. [7]), andafter analysis-deposited in Mendeley Datasets repository, which is accessible at URL: [https://data.mendeley.com/datasets/4rsz3snvk5/3] (dataset ref. [8]). The sequence reads were analyzed by bioinformatics tools [9-12]. The assembled consensus contigs revealed Nb orthologs of diverse Ag-specificities, including those isolated by conventional panning and Sangersequenced functional Nbs. Contig 1 CDR1-3 matched to those of anti-Trypanosoma evansi RoTat1.2 variant surface glycoprotein (VSG), while Contig 2 CDR1-3 matched to those of anti-LPS Nb clones isolated from the library. Contig 3 was however incomplete and lacked CDR3. Despite lacking the depth, the NGS data is a useful guide for selection of antigen-specific Nbs from the library, as demonstrated by anti-T. evansi VSG Nbs, and provides templates for Nb-based diagnostic reagents and therapeutic agents. (c) 2020 The Authors. PublishedbyElsevierInc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
类型	Article ; Data Paper
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000617525400051
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352782
作者单位	[Banerjee, Somesh; Singh, Ajit; Rawat, Jagveer] Dept Vet Microbiol, Immunol Sect, Hisar, Haryana, India; [Bansal, Nitish] Dept Vet Publ Helth & Epidemiol, Hisar, Haryana, India; [Bansal, Nitish; Maan, Sushila] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Anim Biotechnol, Hisar 125004, Haryana, India
推荐引用方式 GB/T 7714	Banerjee, Somesh,Singh, Ajit,Rawat, Jagveer,et al. Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel (Camelus dromedarius L.)[J],2021,34.
APA	Banerjee, Somesh,Singh, Ajit,Rawat, Jagveer,Bansal, Nitish,&Maan, Sushila.(2021).Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel (Camelus dromedarius L.).DATA IN BRIEF,34.
MLA	Banerjee, Somesh,et al."Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel (Camelus dromedarius L.)".DATA IN BRIEF 34(2021).