干旱区生态环境知识资源中心(Arid): Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal -derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction

Arid

DOI	10.3724/SP.J.1123.2020.05012
	Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal -derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction
	Liu Bolin; Xie Jian; Zhao Ziwei; Wang Xiuli; Shuan Xiaomei
通讯作者	Liu, BL (corresponding author), Anhui Prov Ctr Dis Control & Prevent, Hefei 230601, Peoples R China.
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2021
卷号	39 期号:4 页码:406-414
英文摘要	Chloramphenicols, nitroimidazoles, lincosamides, and macrolides are common antibiotics used in veterinary medicine. Overdoses of these drugs will lead to residual substances in animal -derived foods and accumulate in the body through the food chain, thereby exerting adverse effects on human health. Therefore, regulation of veterinary drug levels is imperative to ensure the quality of animal -derived foods and safeguard the health of consumers. In this study, a method based on ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction ( SPE-UPLC-MS/MS) was developed for the simultaneous determination of eight prohibited and restricted veterinary drugs and three metabolite residues across four categories ( chloramphenicols, nitroimidazoles, lincosamides, and macrolides) in eggs, liquid milk, chicken, and freshwater fish. The main factors affecting the response, recovery, and sensitivity of the method, such as the type and pH values of the extraction solvent, dilution solution for the analytes, type of chromatographic column, and type and proportion of the mobile phase, were optimized during sample pretreatment and instrument analysis. The samples were hydrolyzed and dispersed in 0. 1 mol/L phosphate buffer solutions ( pH 9. 0) and extracted with acetonitrile. The extract was further extracted using ethyl acetate. After centrifugation, the supernatant ethyl acetate was concentrated to near dryness in nitrogen below 40 C. The residue was dissolved in 0. 3 mL methanol, followed by the addition of 5. 7 mL phosphate buffer solution. After shaking, the solutions were purified and enriched on an Oasis HLB SPE column. The target analytes were separated on an ACQUITY UPLC BEH C18 chromatographic column (100 mmx2. 1 mm, 1. 7 m) at a column temperature of 40 C with a flow rate of 0. 4 mL/min. The injection volume was 10 pi, L. Gradient elution was carried out with methanol and 0. 1% formic acid aqueous solution as the mobile phases. Multiple reaction monitoring ( MRM) was conducted in the positive and negative electrospray ionization modes. The isotope internal standard method was used for quantitative analysis. Under optimal conditions, each analyte showed a good linear relationship in each range, and the correlation coefficient (R2) was greater than 0. 99. The limits of detection ( LODs) ranged from 0. 050 to 0. 50 g/kg, and the limits of quantification ( LOQs) ranged from 0. 20 to 1. 5 g/kg. With eggs, freshwater fish, chicken, and liquid milk as the matrix samples, the recoveries in spiked blank samples were determined at different addition levels in compliance with the current legislation. The average recoveries of the 11 analytes were 65. 3% to 108%. The relative standard deviations ( RSDs) were between 0. 40% and 21%. The matrix effects of the analytes were between 0. 012 4% and 46. 80% in four different samples after purification on the Oasis HLB column. The practicality of the proposed approach for routine analyses of the eight prohibited and restricted veterinary drugs, and three metabolite residuals was evaluated by applying it to the determination of these compounds in animal -derived food samples. The samples, including 80 eggs, 80 chicken, 40 liquid milk, and 32 freshwater fish, were procured from a supermarket and a farm product market. The results of the positive samples were consistent with those observed with the standard methods. The method described herein is easy to operate, sensitive, and accurate. It is suitable for the simultaneous and rapid determination of various prohibited and restricted veterinary drug residues and metabolites in animal -derived foods.
英文关键词	ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) solid phase extraction (SPE) matrix effect (ME) isotope internal standard veterinary drug residues metabolites animal -derived foods
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000645055000008
WOS关键词	RESIDUES ; PRODUCTS
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352760
作者单位	[Liu Bolin; Xie Jian; Zhao Ziwei; Wang Xiuli; Shuan Xiaomei] Anhui Prov Ctr Dis Control & Prevent, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Liu Bolin,Xie Jian,Zhao Ziwei,et al. Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal -derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction[J],2021,39(4):406-414.
APA	Liu Bolin,Xie Jian,Zhao Ziwei,Wang Xiuli,&Shuan Xiaomei.(2021).Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal -derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction.CHINESE JOURNAL OF CHROMATOGRAPHY,39(4),406-414.
MLA	Liu Bolin,et al."Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal -derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction".CHINESE JOURNAL OF CHROMATOGRAPHY 39.4(2021):406-414.