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DOI | 10.2478/auoc-2020-0020 |
Optimization of SPE method for the extraction of 12 neurotransmitters from sheep brain | |
Tampu, Raluca; Tampu, Catalin; Elfakir, Claire | |
通讯作者 | Tampu, C (corresponding author), Vasile Alecsandri Univ Bacau, Fac Engn, Calea Marasesti 157, Bacau 600115, Romania. |
来源期刊 | OVIDIUS UNIVERSITY ANNALS OF CHEMISTRY
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ISSN | 1583-2430 |
EISSN | 2286-038X |
出版年 | 2020 |
卷号 | 31期号:2页码:110-121 |
英文摘要 | The present paper presents our attempts concerning the development of an extraction method for catecholamines. In order to achieve the extraction of all the selected solutes using a single SPE cartridge, several types of support were tested, among them: cation exchange supports, hydrophilic-lipophilic supports, C18 supports and PGC supports. As unfortunately none of the supports tested offered us the possibility of carrying out the extraction of 12 catecholamines from our standard mixture, we chose to use a coupling of two different cartridges: Oasis HLB and PGC which together ensure the extraction of all the compounds of the mixture with good extraction yields and with simple protocols. The selected cartridges were successfully tested for the extraction of a sample spiked from sheep brain with the 12 catecholamines in our mixture. The SPE method that we have developed allows the purification of the samples (a significant part of the components of the matrix is eliminated during this step) and also a preconcentration of the samples. |
英文关键词 | Catecholamine Oasis HLB PGC SPE Sheep brain extract |
类型 | Article |
语种 | 英语 |
开放获取类型 | DOAJ Gold |
收录类别 | ESCI |
WOS记录号 | WOS:000616969300001 |
WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; TANDEM MASS-SPECTROMETRY ; POROUS GRAPHITIC CARBON ; PLASMA-FREE METANEPHRINE ; CAPILLARY-ELECTROPHORESIS ; CATECHOLAMINE METABOLITES ; HOMOVANILLIC-ACID ; L-DOPA ; SEPARATION |
WOS类目 | Chemistry, Multidisciplinary |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/352657 |
作者单位 | [Tampu, Raluca; Tampu, Catalin] Vasile Alecsandri Univ Bacau, Fac Engn, Calea Marasesti 157, Bacau 600115, Romania; [Elfakir, Claire] Univ Orleans, CNRS UMR 7311, Inst Organ & Analyt Chem, BP 6759, F-45067 Orleans, France |
推荐引用方式 GB/T 7714 | Tampu, Raluca,Tampu, Catalin,Elfakir, Claire. Optimization of SPE method for the extraction of 12 neurotransmitters from sheep brain[J],2020,31(2):110-121. |
APA | Tampu, Raluca,Tampu, Catalin,&Elfakir, Claire.(2020).Optimization of SPE method for the extraction of 12 neurotransmitters from sheep brain.OVIDIUS UNIVERSITY ANNALS OF CHEMISTRY,31(2),110-121. |
MLA | Tampu, Raluca,et al."Optimization of SPE method for the extraction of 12 neurotransmitters from sheep brain".OVIDIUS UNIVERSITY ANNALS OF CHEMISTRY 31.2(2020):110-121. |
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