Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.bcab.2020.101827 |
ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Marechal) | |
Bhadkaria, Amita; Gupta, Neha; Narvekar, Dakshita Tanaji; Bhadkariya, Rajni; Saral, Anamika; Srivastava, Nidhi; Koul, Kirtee Kumar; Bhagyawant, Sameer Suresh | |
通讯作者 | Bhagyawant, SS (corresponding author), Jiwaji Univ, Sch Studies Biotechnol, Gwalior, Madhya Pradesh, India. |
来源期刊 | BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
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EISSN | 1878-8181 |
出版年 | 2020 |
卷号 | 30 |
英文摘要 | Moth bean (Vigna aconitifolia (Jacq.) Marechal) is considered as one of the underutilised food legume vis-avis the Indian sub-continent. The seeds of this legume are a rich source of soluble proteins for nutrition. PCR-based marker characterizations for assessing genetic diversity are almost negligible in this crop. Present investigation, there fore, employed inter simple sequence repeat (ISSR) markers to assess the genetic diversity amongst 25 accessions. The di and penta nucleotide repeat markers showed higher polymorphism on amplification than those observed using trinucleotide markers. ISSR marker UBC-811 detected 36.15% polymorphism while UBC-879 detected 61.03% with an average of 9.7% on each primer. Based on dendrogram analysis, a logical similarity be tween molecular data and geographical origin was corroborated. The accessions cultivated in west ern coast of India were closely related than north-western and arid regions of India. The accessions like Maru, Jadiya and Jawala can be recommended as parents for further breeding pro grammes to procure additional variation complexes in moth bean genetic stocks. |
英文关键词 | Moth bean Underutilised legume ISSR Vigna species Geographic distance |
类型 | Article |
语种 | 英语 |
收录类别 | ESCI |
WOS记录号 | WOS:000600384500001 |
WOS类目 | Biotechnology & Applied Microbiology |
WOS研究方向 | Biotechnology & Applied Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/352519 |
作者单位 | [Bhadkaria, Amita; Narvekar, Dakshita Tanaji; Bhadkariya, Rajni; Saral, Anamika; Koul, Kirtee Kumar; Bhagyawant, Sameer Suresh] Jiwaji Univ, Sch Studies Biotechnol, Gwalior, Madhya Pradesh, India; [Gupta, Neha] Rajmata Vijayaraje Scindia Krishi Vishwavidalaya, Plant Mol Biol & Biotechnol Dept, Gwalior, Madhya Pradesh, India; [Srivastava, Nidhi] Banasthali Vidyapeeth, Dept Biosci & Biotechnol, Banasthali, Rajasthan, India |
推荐引用方式 GB/T 7714 | Bhadkaria, Amita,Gupta, Neha,Narvekar, Dakshita Tanaji,et al. ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Marechal)[J],2020,30. |
APA | Bhadkaria, Amita.,Gupta, Neha.,Narvekar, Dakshita Tanaji.,Bhadkariya, Rajni.,Saral, Anamika.,...&Bhagyawant, Sameer Suresh.(2020).ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Marechal).BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY,30. |
MLA | Bhadkaria, Amita,et al."ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Marechal)".BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 30(2020). |
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