干旱区生态环境知识资源中心(Arid): Constitutive expression of two A-5 subgroup DREB transcription factors from desert shrub Ammopiptanthus mongolicus confers different stress tolerances in transgenic Arabidopsis

Arid

DOI	10.1007/s11240-021-02128-w
	Constitutive expression of two A-5 subgroup DREB transcription factors from desert shrub Ammopiptanthus mongolicus confers different stress tolerances in transgenic Arabidopsis
	Wang, Xuefeng; Ma, Li; Zhang, Huiling; Zhang, Yu; Xue, Min; Ren, Meiyan; Tang, Kuangang; Guo, Huiqin; Wang, Maoyan
通讯作者	Wang, MY (corresponding author), Inner Mongolia Agr Univ, Coll Life Sci, 29 East Erdos St, Hohhot 010011, Peoples R China.
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2021
英文摘要	Dehydration-responsive element binding proteins (DREBs) constitute a large subfamily in the plant-specific APETALA2/ethylene-responsive factor (ERF) family and play crucial roles in plant stress tolerance. So far, studies on the A-5 subgroup DREBs which comprise the largest proportion of the DREB subfamily are limited. Here, we report functional characterization of two A-5 subgroup genes named AmDREB1 and AmDREB2 from Ammopiptanthus mongolicus, a desert shrub with high abiotic stress tolerance. Both AmDREB1 and AmDREB2 proteins contain an ERF-associated amphiphilic repression (EAR)-like motif and were localized in the nucleus. The transcription levels of AmDREB1 and AmDREB2 in laboratory-cultured seedlings increased differentially under dehydration, salt, cold and/or heat stresses as well as under abscisic acid (ABA) treatment. In leaves of naturally growing shrubs in the wild, the transcription level of AmDREB2 also increased in response to seasonal cold weather of a year. Moreover, AmDREB1 was most abundantly expressed in leaves, followed by immature pods; AmDREB2 was abundantly expressed in all examined organs. Constitutively expressing AmDREB1 in Arabidopsis significantly enhanced drought and salt tolerances of transgenic lines, likely by inducing the expression of some stress-inducible genes and accumulating more proline and less Na+. Constitutive expression of AmDREB2 conferred only osmotic stress tolerance in transgenic Arabidopsis at the seed germination stage. Both AmDREBs also conferred ABA insensitivity in transgenics. This study provides important evidence that the A-5 subgroup EAR motif-containing DREBs can positively regulate stress response and tolerance in plants, and that AmDREB1 may have potential application in improving drought and salt tolerances in crop plants. Key message AmDREB1 and AmDREB2 were differentially induced by abiotic stressors, exogenous ABA and seasonal cold weather. The tolerances of their transgenics to drought and/or salt as well as ABA were enhanced.
英文关键词	Ammopiptanthus mongolicus DREB transcription factor Gene expression Transgenics Drought and salt ABA
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000664014400001
WOS关键词	MOSS SYNTRICHIA-CANINERVIS ; SALT TOLERANCE ; GENE-EXPRESSION ; ABSCISIC-ACID ; EAR-MOTIF ; DROUGHT ; COLD ; DEHYDRATION ; ABA ; REPRESSOR
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352433
作者单位	[Ma, Li; Zhang, Huiling; Zhang, Yu; Xue, Min; Ren, Meiyan; Tang, Kuangang; Guo, Huiqin; Wang, Maoyan] Inner Mongolia Agr Univ, Coll Life Sci, 29 East Erdos St, Hohhot 010011, Peoples R China; [Wang, Xuefeng] Inner Mongolia Agr Univ, Vocat & Tech Coll, Baotou 014109, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuefeng,Ma, Li,Zhang, Huiling,et al. Constitutive expression of two A-5 subgroup DREB transcription factors from desert shrub Ammopiptanthus mongolicus confers different stress tolerances in transgenic Arabidopsis[J],2021.
APA	Wang, Xuefeng.,Ma, Li.,Zhang, Huiling.,Zhang, Yu.,Xue, Min.,...&Wang, Maoyan.(2021).Constitutive expression of two A-5 subgroup DREB transcription factors from desert shrub Ammopiptanthus mongolicus confers different stress tolerances in transgenic Arabidopsis.PLANT CELL TISSUE AND ORGAN CULTURE.
MLA	Wang, Xuefeng,et al."Constitutive expression of two A-5 subgroup DREB transcription factors from desert shrub Ammopiptanthus mongolicus confers different stress tolerances in transgenic Arabidopsis".PLANT CELL TISSUE AND ORGAN CULTURE (2021).