干旱区生态环境知识资源中心(Arid): Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions

Arid

DOI	10.1007/s00299-020-02640-x
	Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions
	Rashid, Urooj; Yasmin, Humaira; Hassan, Muhammad Nadeem; Naz, Rabia; Nosheen, Asia; Sajjad, Muhammad; Ilyas, Noshin; Keyani, Rumana; Jabeen, Zahra; Mumtaz, Saqib; Alyemeni, Mohammed Nasser; Ahmad, Parvaiz
通讯作者	Yasmin, H (corresponding author), COMSATS Univ Islamabad CUI, Dept Biosci, Islamabad 45550, Pakistan.
来源期刊	PLANT CELL REPORTS
ISSN	0721-7714
EISSN	1432-203X
出版年	2021-01
英文摘要	Key message A detailed study of the response of wheat plants, inoculated with drought-tolerant PGPR is studied which would be beneficial to achieve genetic improvement of wheat for drought tolerance. Drought stress, a major challenge under current climatic conditions, adversely affects wheat productivity. In the current study, we observed the response of wheat plants, inoculated with drought-tolerant plant growth-promoting rhizobacteria (PGPR) Bacillus megaterium (MU2) and Bacillus licheniformis (MU8) under induced drought stress. In vitro study of 90 rhizobacteria exhibited 38 isolates showed one or more plant growth-promoting properties, such as solubilization of phosphorus, potassium, and exopolysaccharide production. Four strains revealing the best activities were tested for their drought-tolerance ability by growing them on varying water potentials (- 0.05 to - 0.73 MPa). Among them, two bacterial strains Bacillus megaterium and Bacillus licheniformis showed the best drought-tolerance potential, ACC deaminase activities, IAA production, and antagonistic activities against plant pathogens. Additionally, these strains when exposed to drought stress (- 0.73 MPa) revealed the induction of three new polypeptides (18 kDa, 35 kDa, 30 kDa) in Bacillus megaterium. We determined that 10(6) cells/mL of Bacillus megaterium and Bacillus licheniformis were enough to induce drought tolerance in wheat under drought stress. These drought-tolerant strains increased the germination index (11-46%), promptness index (16-50%), seedling vigor index (11-151%), fresh weight (35-192%), and dry weight (58-226%) of wheat under irrigated and drought stress. Moreover, these strains efficiently colonized the wheat roots and increased plant biomass, relative water content, photosynthetic pigments, and osmolytes. Upon exposure to drought stress, Bacillus megaterium inoculated wheat plants exhibited improved tolerance by enhancing 59% relative water content, 260, 174 and 70% chlorophyll a, b and carotenoid, 136% protein content, 117% proline content and 57% decline in MDA content. Further, activities of defense-related antioxidant enzymes were also upregulated. Our results revealed that drought tolerance was more evident in Bacillus megaterium as compared to Bacillus licheniformis. These strains could be effective bioenhancer and biofertilizer for wheat cultivation in arid and semi-arid regions. However, a detailed study at the molecular level to deduce the mechanism by which these strains alleviate drought stress in wheat plants needs to be explored.
英文关键词	Drought Proline MDA Antioxidant enzymes Plant growth-promoting rhizobacteria (PGPR) Wheat
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000605908600001
WOS关键词	GROWTH-PROMOTING RHIZOBACTERIA ; DEAMINASE PRODUCING PGPR ; STRESS ; SOIL ; PROLINE ; PLANTS ; YIELD
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352432
作者单位	[Rashid, Urooj; Yasmin, Humaira; Hassan, Muhammad Nadeem; Naz, Rabia; Nosheen, Asia; Sajjad, Muhammad; Keyani, Rumana; Jabeen, Zahra; Mumtaz, Saqib] COMSATS Univ Islamabad CUI, Dept Biosci, Islamabad 45550, Pakistan; [Ilyas, Noshin] PMAS Arid Univ Rawalpindi, Dept Bot, Rawalpindi 46300, Pakistan; [Alyemeni, Mohammed Nasser; Ahmad, Parvaiz] King Saud Univ, Coll Sci, Bot & Microbiol Dept, Riyadh, Saudi Arabia; [Ahmad, Parvaiz] SP Coll, Dept Bot, Srinagar, Jammu & Kashmir, India
推荐引用方式 GB/T 7714	Rashid, Urooj,Yasmin, Humaira,Hassan, Muhammad Nadeem,et al. Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions[J]. King Saud University,2021.
APA	Rashid, Urooj.,Yasmin, Humaira.,Hassan, Muhammad Nadeem.,Naz, Rabia.,Nosheen, Asia.,...&Ahmad, Parvaiz.(2021).Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions.PLANT CELL REPORTS.
MLA	Rashid, Urooj,et al."Drought-tolerant Bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions".PLANT CELL REPORTS (2021).