干旱区生态环境知识资源中心(Arid): Diversity and Spatiotemporal Dynamics of Fungal Communities in the Rhizosphere Soil of Cotton in the Arid Region of Northwest China

Arid

DOI	10.1007/s00248-020-01646-y
	Diversity and Spatiotemporal Dynamics of Fungal Communities in the Rhizosphere Soil of Cotton in the Arid Region of Northwest China
	Shi, YingWu; Yang, HongMei; Chu, Ming; Niu, XinXiang; Huo, XiangDong; Gao, Yan; Zeng, Jun; Lin, Qing; Lou, Kai
通讯作者	Shi, YW (corresponding author), Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Xinjiang, Peoples R China. ; Shi, YW (corresponding author), Xinjiang Lab Special Environm Microbiol, Urumqi 830091, Xinjiang, Peoples R China. ; Shi, YW (corresponding author), Minist Agr & Countryside, Key Lab Agr Environm Northwest Oasis, Urumqi 830091, Xinjiang, Peoples R China. ; Shi, YW (corresponding author), Xinjiang Acad Agr Sci, Inst Soil Fertilizer & Agr Water Conservat, Urumqi 830091, Xinjiang, Peoples R China.
来源期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
EISSN	1432-184X
出版年	2021
英文摘要	This study aimed to investigate the fungal diversity and its temporal and spatial dynamics in the rhizosphere soil of healthy cotton by high-throughput sequencing. We studied species richness, composition, and distribution of cotton rhizosphere fungal community with respect to location (Alaer, Kuerle, Tumushuke, Hami, Shihezi, Wusu, and Jinghe) and plant growth period (seedling stage, bud stage, flowering stage, and boll-opening stage) using the methods of PCR-based high-throughput sequencing and real-time quantitative PCR. A total of 1,838,454 fungal nuclear ribosomal internal transcribed spacer region sequences (rRNA ITS) were obtained from all cotton plants sampled at different growth stages in the seven locations in Xinjiang. The most abundant fungal group in the cotton rhizosphere was the Ascomycota (78.72%), followed by the Zygomycota (9.56%) and Basidiomycota (2.77%). These sequences revealed an enormous number of operational taxonomic units (OTUs) in cotton (1802 unique OTUs), with 67-464 OTUs in a single cotton sample, at a 3% threshold and a sequencing depth of 30,000 sequences. We identified 33 classes and 389 genera from the resulting 1,800,714 sequences. Sordariomycetes was the most frequent class in all samples, followed by Leotiomycetes and Eurotiomycetes. There were some differences in OTUs among different growth stages, but the differences were not significant, with 382 OTUs (14.66%) being common to each of the stages. A marked difference in the diversity of fungi in the rhizosphere soil of cotton was evident among the different locations, with the highest number of OTUs being detected in Jinghe (1084 OTUs) and clusters of OTUs representative of northern and eastern Xinjiang being detected. There were significantly more tags of Mortierella in Jinghe and Wusu than in the other sampling sites. The dynamics of the rhizosphere fungal communities were influenced by sampling sites. To the best of our knowledge, the current study is the first application of PCR-based Illumina to characterize and compare the fungal biodiversity in multiple rhizosphere soil samples from cotton.
英文关键词	Cotton Rhizosphere fungi Verticillium wilt Diversity Dynamics
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000605852200006
WOS关键词	BACTERIAL COMMUNITY ; MICROBIAL DIVERSITY ; PLANT-AGE ; ENDOPHYTES ; IMPACT ; GROWTH ; POPULATIONS ; VARIETIES ; RICE ; PCR
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352389
作者单位	[Shi, YingWu; Yang, HongMei; Chu, Ming; Huo, XiangDong; Gao, Yan; Zeng, Jun; Lin, Qing; Lou, Kai] Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Xinjiang, Peoples R China; [Shi, YingWu; Yang, HongMei; Chu, Ming; Huo, XiangDong; Gao, Yan; Zeng, Jun; Lin, Qing; Lou, Kai] Xinjiang Lab Special Environm Microbiol, Urumqi 830091, Xinjiang, Peoples R China; [Shi, YingWu; Yang, HongMei; Chu, Ming; Niu, XinXiang] Minist Agr & Countryside, Key Lab Agr Environm Northwest Oasis, Urumqi 830091, Xinjiang, Peoples R China; [Shi, YingWu; Niu, XinXiang] Xinjiang Acad Agr Sci, Inst Soil Fertilizer & Agr Water Conservat, Urumqi 830091, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Shi, YingWu,Yang, HongMei,Chu, Ming,et al. Diversity and Spatiotemporal Dynamics of Fungal Communities in the Rhizosphere Soil of Cotton in the Arid Region of Northwest China[J],2021.
APA	Shi, YingWu.,Yang, HongMei.,Chu, Ming.,Niu, XinXiang.,Huo, XiangDong.,...&Lou, Kai.(2021).Diversity and Spatiotemporal Dynamics of Fungal Communities in the Rhizosphere Soil of Cotton in the Arid Region of Northwest China.MICROBIAL ECOLOGY.
MLA	Shi, YingWu,et al."Diversity and Spatiotemporal Dynamics of Fungal Communities in the Rhizosphere Soil of Cotton in the Arid Region of Northwest China".MICROBIAL ECOLOGY (2021).