干旱区生态环境知识资源中心(Arid): A leucoanthocyanidin dioxygenase gene (RtLDOX2) from the feral forage plant Reaumuria trigyna promotes the accumulation of flavonoids and improves tolerance to abiotic stresses

Arid

DOI	10.1007/s10265-021-01315-2
	A leucoanthocyanidin dioxygenase gene (RtLDOX2) from the feral forage plant Reaumuria trigyna promotes the accumulation of flavonoids and improves tolerance to abiotic stresses
	Li, Ningning; Wang, Xue; Ma, Binjie; Wu, Zhigang; Zheng, Linlin; Qi, Zhi; Wang, Yingchun
通讯作者	Wang, YC (corresponding author), Inner Mongolia Univ, Key Lab Herbage & Endem Crop Biol, Minist Educ, State Key Lab Reprod Regulat & Breeding Grassland, 24 Zhaojun Rd, Hohhot 010070, Peoples R China. ; Wang, YC (corresponding author), Inner Mongolia Univ, Coll Life Sci, 24 Zhaojun Rd, Hohhot 010070, Peoples R China.
来源期刊	JOURNAL OF PLANT RESEARCH
ISSN	0918-9440
EISSN	1618-0860
出版年	2021
英文摘要	Reaumuria trigyna, a Tamaricaceae archaic recretohalophyte, is an important feral forage plant in the desert steppe of northwestern China. We identified two significantly differentially expressed leucoanthocyanidin dioxygenase genes (RtLDOX/RtLDOX2) and investigated the function and characteristics of RtLDOX2. RtLDOX2 from R. trigyna was rapidly upregulated by salt, drought, and abscisic acid, consistent with the stress-related cis-regulatory elements in the promoter region. Recombinant RtLDOX2 converted dihydrokaempferol to kaempferol in vitro, and was thus interchangeable with flavonol synthase, a dioxygenase in the flavonoid pathway. Transgenic plants overexpressing RtLDOX2 accumulated more anthocyanin and flavonols under abiotic stresses, speculating that RtLDOX2 may act as a multifunctional dioxygenase in the synthesis of anthocyanins and flavonols. Overexpression of RtLDOX2 enhanced the primary root length, biomass accumulation, and chlorophyll content of salt-, drought-, and ultraviolet-B-stressed transgenic Arabidopsis. Antioxidant enzyme activity; proline content; and expression of antioxidant enzyme, proline biosynthesis, and ion-transporter genes were increased in transgenic plants. Therefore, RtLDOX2 confers tolerance to abiotic stress on transgenic Arabidopsis by promoting the accumulation of anthocyanins and flavonols. This in turn increases reactive oxygen species scavenging and activates other stress responses, such as osmotic adjustment and ion transport, and so improves tolerance to abiotic stresses.
英文关键词	Abiotic stress Anthocyanins Flavonols Leucoanthocyanidin dioxygenase Reaumuria trigyna
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000654831400001
WOS关键词	FLAVANONE 3-HYDROXYLASE GENE ; ANTHOCYANIDIN SYNTHASE GENE ; SALT-STRESS ; MOLECULAR-CLONING ; EXPRESSION ANALYSIS ; TRANSGENIC RICE ; COLORFUL MODEL ; DROUGHT STRESS ; BIOSYNTHESIS ; OVEREXPRESSION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352341
作者单位	[Li, Ningning] Inner Mongolia Agr Univ, Coll Agr, Hohhot 010019, Peoples R China; [Li, Ningning; Wang, Xue; Ma, Binjie; Wu, Zhigang; Zheng, Linlin; Qi, Zhi; Wang, Yingchun] Inner Mongolia Univ, Key Lab Herbage & Endem Crop Biol, Minist Educ, State Key Lab Reprod Regulat & Breeding Grassland, 24 Zhaojun Rd, Hohhot 010070, Peoples R China; [Li, Ningning; Wang, Xue; Ma, Binjie; Wu, Zhigang; Zheng, Linlin; Qi, Zhi; Wang, Yingchun] Inner Mongolia Univ, Coll Life Sci, 24 Zhaojun Rd, Hohhot 010070, Peoples R China
推荐引用方式 GB/T 7714	Li, Ningning,Wang, Xue,Ma, Binjie,et al. A leucoanthocyanidin dioxygenase gene (RtLDOX2) from the feral forage plant Reaumuria trigyna promotes the accumulation of flavonoids and improves tolerance to abiotic stresses[J],2021.
APA	Li, Ningning.,Wang, Xue.,Ma, Binjie.,Wu, Zhigang.,Zheng, Linlin.,...&Wang, Yingchun.(2021).A leucoanthocyanidin dioxygenase gene (RtLDOX2) from the feral forage plant Reaumuria trigyna promotes the accumulation of flavonoids and improves tolerance to abiotic stresses.JOURNAL OF PLANT RESEARCH.
MLA	Li, Ningning,et al."A leucoanthocyanidin dioxygenase gene (RtLDOX2) from the feral forage plant Reaumuria trigyna promotes the accumulation of flavonoids and improves tolerance to abiotic stresses".JOURNAL OF PLANT RESEARCH (2021).