干旱区生态环境知识资源中心(Arid): Diversity and abundance of culturable nitrogen-fixing bacteria in the phyllosphere of maize

Arid

DOI	10.1111/jam.14975
	Diversity and abundance of culturable nitrogen-fixing bacteria in the phyllosphere of maize
	Abadi, V. A. J. M.; Sepehri, M.; Rahmani, H. A.; Dolatabad, H. K.; Shamshiripour, M.; Khatabi, B.
通讯作者	Sepehri, M (corresponding author), Shiraz Univ, Sch Agr, Dept Soil Sci, Shiraz, Iran.
来源期刊	JOURNAL OF APPLIED MICROBIOLOGY
ISSN	1364-5072
EISSN	1365-2672
出版年	2021
英文摘要	Aims The present study aimed at gaining an insight into the abundance and genetic diversity of culturable N-fixing epiphyte bacteria on the phyllosphere of maize in arid and semi-arid regions of Iran. Methods and Results Leaf samples of the maize variety, 'single cross 704' (Zea mays L.) were collected from different locations in Iran. The community of culturable N-fixing epiphyte bacteria present was examined by 16S rRNA sequencing, BOXAIR-polymerase chain reaction (PCR) and restricted fragment length polymorphisms analysis of 16S rRNA gene (16S-RFLP). Approximately, 31 center dot 82% of the 242 isolates were identified as N-fixers by cultivation of bacteria in Rennie medium and detection of their nifH gene. The N-fixers were affiliated with four bacterial phyla: Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. 16S rRNA sequencing detected 16 genera and 24 different species in the identified phyla. The most dominant genus was Bacillus and the species identified were B. pumilus, B. amyloliquefaciens, B. subtilis, B. paralicheniformis, B. licheniformis, B. niabensis and B. megaterium. In total, 22 RFLP groups were present among the isolates originally identified as N-fixing bacteria. BOXAIR-PCR showed that there was a low similarity level among the N-fixing bacteria isolates, and genetic differentiation of individual strains was relatively great. Conclusions Our findings suggest that nitrogen-fixing epiphyte bacteria on the phyllosphere of maize may provide significant nitrogen input into arid and semi-arid ecosystem. Significance and Impact of the Study This research implies that phyllosphere epiphyte diazotrophs have much to offer in sustainable agriculture and can be an alternative to chemical N-fertilizers for providing nitrogen to crops arid and semi-arid regions.
英文关键词	16S‐ RFLP BOXAIR‐ PCR nifH gene nitrogen‐ fixing bacteria phyllo‐ microbiome
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000604352100001
WOS关键词	PLANT-GROWTH ; DIAZOTROPHIC BACTERIA ; ENDOPHYTIC BACTERIA ; GENETIC DIVERSITY ; COMMUNITY ; RICE ; RHIZOBACTERIA ; MICROBIOLOGY ; PHYLOGENY ; SOFTWARE
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352303
作者单位	[Abadi, V. A. J. M.; Sepehri, M.] Shiraz Univ, Sch Agr, Dept Soil Sci, Shiraz, Iran; [Rahmani, H. A.; Dolatabad, H. K.; Shamshiripour, M.] Agr Res Educ & Extens Org, Soil & Water Res Inst, Karaj, Iran; [Khatabi, B.] Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Princess Anne, MD USA
推荐引用方式 GB/T 7714	Abadi, V. A. J. M.,Sepehri, M.,Rahmani, H. A.,et al. Diversity and abundance of culturable nitrogen-fixing bacteria in the phyllosphere of maize[J],2021.
APA	Abadi, V. A. J. M.,Sepehri, M.,Rahmani, H. A.,Dolatabad, H. K.,Shamshiripour, M.,&Khatabi, B..(2021).Diversity and abundance of culturable nitrogen-fixing bacteria in the phyllosphere of maize.JOURNAL OF APPLIED MICROBIOLOGY.
MLA	Abadi, V. A. J. M.,et al."Diversity and abundance of culturable nitrogen-fixing bacteria in the phyllosphere of maize".JOURNAL OF APPLIED MICROBIOLOGY (2021).