干旱区生态环境知识资源中心(Arid): A micro-CT-based standard brain atlas of the bumblebee

Arid

DOI	10.1007/s00441-021-03482-z
	A micro-CT-based standard brain atlas of the bumblebee
	Rother, Lisa; Kraft, Nadine; Smith, Dylan B.; el Jundi, Basil; Gill, Richard J.; Pfeiffer, Keram
通讯作者	Pfeiffer, K (corresponding author), Univ Wurzburg, Dept Behav Physiol & Sociobiol, Bioctr, D-97074 Wurzburg, Germany.
来源期刊	CELL AND TISSUE RESEARCH
ISSN	0302-766X
EISSN	1432-0878
出版年	2021
英文摘要	In recent years, bumblebees have become a prominent insect model organism for a variety of biological disciplines, particularly to investigate learning behaviors as well as visual performance. Understanding these behaviors and their underlying neurobiological principles requires a clear understanding of brain anatomy. Furthermore, to be able to compare neuronal branching patterns across individuals, a common framework is required, which has led to the development of 3D standard brain atlases in most of the neurobiological insect model species. Yet, no bumblebee 3D standard brain atlas has been generated. Here we present a brain atlas for the buff-tailed bumblebee Bombus terrestris using micro-computed tomography (micro-CT) scans as a source for the raw data sets, rather than traditional confocal microscopy, to produce the first ever micro-CT-based insect brain atlas. We illustrate the advantages of the micro-CT technique, namely, identical native resolution in the three cardinal planes and 3D structure being better preserved. Our Bombus terrestris brain atlas consists of 30 neuropils reconstructed from ten individual worker bees, with micro-CT allowing us to segment neuropils completely intact, including the lamina, which is a tissue structure often damaged when dissecting for immunolabeling. Our brain atlas can serve as a platform to facilitate future neuroscience studies in bumblebees and illustrates the advantages of micro-CT for specific applications in insect neuroanatomy.
英文关键词	Bombus terrestris Insect standard brain atlas Iterative shape averaging Neuropils Reconstruction
类型	Article ; Early Access
语种	英语
开放获取类型	Green Published, hybrid
收录类别	SCI-E
WOS记录号	WOS:000667630700001
WOS关键词	AVERAGE-SHAPE ATLAS ; MUSHROOM BODIES ; CENTRAL COMPLEX ; DESERT LOCUST ; VISUAL INPUT ; BEE ; IMPLEMENTATION ; VISUALIZATION ; ENVIRONMENTS ; REGISTRATION
WOS类目	Cell Biology
WOS研究方向	Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352096
作者单位	[Rother, Lisa; Kraft, Nadine; el Jundi, Basil; Pfeiffer, Keram] Univ Wurzburg, Dept Behav Physiol & Sociobiol, Bioctr, D-97074 Wurzburg, Germany; [Smith, Dylan B.; Gill, Richard J.] Imperial Coll London, Dept Life Sci, Silwood Pk,Buckhurst Rd, Ascot SL5 7PY, Berks, England
推荐引用方式 GB/T 7714	Rother, Lisa,Kraft, Nadine,Smith, Dylan B.,et al. A micro-CT-based standard brain atlas of the bumblebee[J],2021.
APA	Rother, Lisa,Kraft, Nadine,Smith, Dylan B.,el Jundi, Basil,Gill, Richard J.,&Pfeiffer, Keram.(2021).A micro-CT-based standard brain atlas of the bumblebee.CELL AND TISSUE RESEARCH.
MLA	Rother, Lisa,et al."A micro-CT-based standard brain atlas of the bumblebee".CELL AND TISSUE RESEARCH (2021).