干旱区生态环境知识资源中心(Arid): Actinomycetes isolates of arid zone of Indian Thar Desert and efficacy of their bioactive compounds against human pathogenic bacteria

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DOI	10.1007/s42977-021-00073-5
	Actinomycetes isolates of arid zone of Indian Thar Desert and efficacy of their bioactive compounds against human pathogenic bacteria
	Kumar, Surendra; Solanki, Dilip Singh; Parihar, Khushbu; Tak, Alkesh; Gehlot, Praveen; Pathak, Rakesh; Singh, Sunil Kumar
通讯作者	Gehlot, P (corresponding author), Jai Narain Vyas Univ, Dept Bot, Mycol & Microbiol Lab, Jodhpur 342001, Rajasthan, India.
来源期刊	BIOLOGIA FUTURA
ISSN	2676-8615
EISSN	2676-8607
出版年	2021
英文摘要	Twenty-six morphotypes of actinomycetes bacteria were isolated from the soils of arid zone of Indian Thar desert, Rajasthan. A significant and positive correlation was found between density of actinomycetes isolates and availability of nitrogen in sandy soil of arid zone suggesting the influence of soil nitrogen on occurrence and propagation of actinomycetes in this region. Molecular identification based on 16S rRNA gene sequencing revealed that the bacterial isolates belong to four actinomycetes genera, viz. Streptomyces (22 species), Nocardiopsis (two species), Saccharomonospora (one species) and Actinoalloteichus (one species). The preliminary screening of 26 isolates against five human pathogenic bacteria, viz. Escherichia coli, Vibrio cholera, Salmonella enterica typhimurium, Staphylococcus aureus and Enterococcus faecalis, showed that only four isolates, viz. Streptomyces sp. (ITD-27), S. enissocaesilis (ITD-29), S. Malachitospinus (ITD-35) and Streptomyces sp. (ITD-47), had antibacterial activity. The secondary screening of these four isolates revealed that the isolate S. malachitospinus (ITD-35) showed the maximum growth inhibition zone and inhibited the growth of all tested gram-positive and gram-negative pathogenic bacteria. Gas chromatography-mass spectrometry analysis of S. malachitospinus (ITD-35) cultural filtrate in n-butanol solvent identified three antibacterial compounds of medicinal significance, viz. 3-octanone, neopentyl isothiocyanate and 2-methyl butyl isothiocyanate.
英文关键词	16S rRNA gene Actinomycetes Bioactive compounds Arid zone Indian Thar desert GC– MS
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000616077100002
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
来源机构	ICAR Central Arid Zone Research Institute
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352089
作者单位	[Kumar, Surendra; Solanki, Dilip Singh; Parihar, Khushbu; Tak, Alkesh; Gehlot, Praveen] Jai Narain Vyas Univ, Dept Bot, Mycol & Microbiol Lab, Jodhpur 342001, Rajasthan, India; [Pathak, Rakesh; Singh, Sunil Kumar] ICAR Cent Arid Zone Res Inst, Jodhpur 342003, Rajasthan, India
推荐引用方式 GB/T 7714	Kumar, Surendra,Solanki, Dilip Singh,Parihar, Khushbu,et al. Actinomycetes isolates of arid zone of Indian Thar Desert and efficacy of their bioactive compounds against human pathogenic bacteria[J]. ICAR Central Arid Zone Research Institute,2021.
APA	Kumar, Surendra.,Solanki, Dilip Singh.,Parihar, Khushbu.,Tak, Alkesh.,Gehlot, Praveen.,...&Singh, Sunil Kumar.(2021).Actinomycetes isolates of arid zone of Indian Thar Desert and efficacy of their bioactive compounds against human pathogenic bacteria.BIOLOGIA FUTURA.
MLA	Kumar, Surendra,et al."Actinomycetes isolates of arid zone of Indian Thar Desert and efficacy of their bioactive compounds against human pathogenic bacteria".BIOLOGIA FUTURA (2021).