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DOI	10.1002/arch.21822
	Identification and functional analysis of dsRNases in spotted-wing drosophila, Drosophila suzukii
	Yoon, June-Sun; Ahn, Seung-Joon; Flinn, Christina M.; Choi, Man-Yeon
通讯作者	Choi, MY (corresponding author), ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA.
来源期刊	ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
ISSN	0739-4462
EISSN	1520-6327
出版年	2021
卷号	107 期号:4
英文摘要	RNAi efficiency in insects is different from species to species; some species in Coleoptera are relatively more amenable to RNA interference (RNAi) than other species. One of the major factors is the presence of dsRNA-degrading enzymes, called dsRNases, in saliva, gut, or hemolymph in insects, which degrade the double-stranded RNA (dsRNA) introduced, resulting in the low efficacy of RNAi. In this study, we report a dsRNA-degrading activity in the gut homogenates from the spotted-wing drosophila, Drosophila suzukii, by ex vivo assay. Then, we identified two Drosophila suzukii dsRNase genes, named DrosudsRNase1 and DrosudsRNase2. In silico analysis shows that the gene structures are similar to dsRNases found in other insects. When dsRNases expressed in Sf9 cells were compared for their dsRNA degrading activities, dsRNase1 was more vital than dsRNase2. Both dsRNases were expressed highly and exclusively in the gut compared to the rest of body. Also, they were highly expressed during larval and adult stages but not in embryonic and pupal stages, suggesting the dsRNases protect foreign RNA molecules received during the feeding periods. DsRNase1 was expressed at a higher level in adults, whereas dsRNase2 showed more expression in early larvae. Our study on the tissue and development-specific patterns of dsRNases provides an improved understanding of the RNAi application for the management of D. suzukii.
英文关键词	Drosophila suzukii dsRNase endonuclease RNAi spotted-wing drosophila
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000663942700001
WOS关键词	DOUBLE-STRANDED-RNA ; COLORADO POTATO BEETLE ; NUCLEASE ACTIVITY ; DESERT LOCUST ; INTERFERENCE ; LARVAL ; AEDES ; EXPRESSION ; EFFICIENCY ; ANOPHELES
WOS类目	Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology ; Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352058
作者单位	[Yoon, June-Sun; Flinn, Christina M.; Choi, Man-Yeon] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA; [Yoon, June-Sun; Flinn, Christina M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA; [Ahn, Seung-Joon] Mississippi State Univ, Depr Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA
推荐引用方式 GB/T 7714	Yoon, June-Sun,Ahn, Seung-Joon,Flinn, Christina M.,et al. Identification and functional analysis of dsRNases in spotted-wing drosophila, Drosophila suzukii[J],2021,107(4).
APA	Yoon, June-Sun,Ahn, Seung-Joon,Flinn, Christina M.,&Choi, Man-Yeon.(2021).Identification and functional analysis of dsRNases in spotted-wing drosophila, Drosophila suzukii.ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,107(4).
MLA	Yoon, June-Sun,et al."Identification and functional analysis of dsRNases in spotted-wing drosophila, Drosophila suzukii".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 107.4(2021).