干旱区生态环境知识资源中心(Arid): Potential targeting of Hep3B liver cancer cells by lupeol isolated from Avicennia marina

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DOI	10.1002/ardp.202100120
	Potential targeting of Hep3B liver cancer cells by lupeol isolated from Avicennia marina
	Eldohaji, Leen M.; Fayed, Bahgat; Hamoda, Alshaimaa M.; Ershaid, Mai; Abdin, Shifaa; Alhamidi, Tasneem B.; Mohammad, Mohammad G.; Omar, Hany A.; Soliman, Sameh S. M.
通讯作者	Soliman, SSM (corresponding author), Univ Sharjah, Dept Med Chem, Coll Pharm, Sharjah 27272, U Arab Emirates.
来源期刊	ARCHIV DER PHARMAZIE
ISSN	0365-6233
EISSN	1521-4184
出版年	2021
英文摘要	Medicinal plants are valuable sources of different active constituents that are known to have important pharmacological activities including anticancer effects. Lupeol, a pentacyclic triterpenoid, present in many medicinal plants, has a wide range of biological activities. Although the anticancer activity of lupeol was reported, the published data are inconsistent and the clear mechanism of action has never been assigned. The current study aims at investigating the anticancer specificity and mechanism of lupeol isolated from Avicennia marina, which grows in the desert of the United Arab Emirates. The compound was purified by chromatography and identified by spectroscopy. Compared with a negative control, lupeol caused significant (p < .001) growth inhibitory activity on MCF-7 and Hep3B parental and resistant cells by 45%, 46%, 72%, and 35%, respectively. The mechanism of action of lupeol was further explored by measuring its effect on key players in cancer development and progression, BCL-2 anti-apoptotic and BAX pro-apoptotic proteins. Lupeol significantly (p < .01) downregulated BCL-2 gene expression in parental and resistant Hep3B cells by 33 and 3.5 times, respectively, contributing to the induction of apoptosis in Hep3B cells, whereas it caused no effect on BAX. Furthermore, the immunoblotting analysis revealed that lupeol cleaved the executioner caspase-3 into its active form. Interestingly, lupeol showed no significant effect on the proliferation of monocytes, whereas it caused an increase in the sub-G1 population and a reduction in the apoptosis rates of monocytes at 48 and 72 h, indicative of no immuno-inflammatory responses. Collectively, lupeol can be considered as promising effective and safe anticancer agent, particularly against Hep3B cancer cells.
英文关键词	anticancer Avicennia marina Hep3B lupeol macrophages
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000657676800001
WOS关键词	INHIBITS GROWTH ; APOPTOTIC DEATH ; IN-VITRO ; PLANT ; SUPPRESSION ; DERIVATIVES ; TRITERPENE ; LEUKEMIA ; LEAVES ; LINES
WOS类目	Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/352054
作者单位	[Eldohaji, Leen M.; Fayed, Bahgat; Hamoda, Alshaimaa M.; Ershaid, Mai; Abdin, Shifaa; Alhamidi, Tasneem B.; Mohammad, Mohammad G.; Omar, Hany A.; Soliman, Sameh S. M.] Univ Sharjah, Res Inst Med & Hlth Sci, Sharjah, U Arab Emirates; [Fayed, Bahgat] Natl Res Ctr, Chem Nat & Microbial Prod Dept, Cairo, Egypt; [Hamoda, Alshaimaa M.] Univ Sharjah, Coll Med, Sharjah, U Arab Emirates; [Hamoda, Alshaimaa M.] Assiut Univ, Dept Pharmacognosy, Fac Pharm, Assiut, Egypt; [Mohammad, Mohammad G.] Univ Sharjah, Dept Med Lab Sci, Coll Hlth Sci, Sharjah, U Arab Emirates; [Omar, Hany A.; Soliman, Sameh S. M.] Univ Sharjah, Coll Pharm, Sharjah, U Arab Emirates
推荐引用方式 GB/T 7714	Eldohaji, Leen M.,Fayed, Bahgat,Hamoda, Alshaimaa M.,et al. Potential targeting of Hep3B liver cancer cells by lupeol isolated from Avicennia marina[J],2021.
APA	Eldohaji, Leen M..,Fayed, Bahgat.,Hamoda, Alshaimaa M..,Ershaid, Mai.,Abdin, Shifaa.,...&Soliman, Sameh S. M..(2021).Potential targeting of Hep3B liver cancer cells by lupeol isolated from Avicennia marina.ARCHIV DER PHARMAZIE.
MLA	Eldohaji, Leen M.,et al."Potential targeting of Hep3B liver cancer cells by lupeol isolated from Avicennia marina".ARCHIV DER PHARMAZIE (2021).