干旱区生态环境知识资源中心(Arid): Babesia bicornis, Theileria bicornis and Theileria equi in metapopulations of two black rhinoceros (Diceros bicornis) subspecies in South Africa and their potential impact on conservation

Arid

DOI	10.1016/j.ttbdis.2020.101635
	Babesia bicornis, Theileria bicornis and Theileria equi in metapopulations of two black rhinoceros (Diceros bicornis) subspecies in South Africa and their potential impact on conservation
	Zimmermann, David E.; Penzhorn, Barend L.; Vorster, Ilse; Troskie, Milana; Oosthuizen, Marinda C.
通讯作者	Penzhorn, BL (corresponding author), Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, Ticks & Tick Borne Dis Res Programme, Private Bag X04, ZA-0110 Onderstepoort, South Africa.
来源期刊	TICKS AND TICK-BORNE DISEASES
ISSN	1877-959X
EISSN	1877-9603
出版年	2021
卷号	12 期号:2
英文摘要	The two black rhinoceros subspecies (Diceros bicornis bicornis and D. b. minor) in South African conservation areas are managed as separate metapopulations. Since infection with Babesia bicornis can be fatal in black rhinoceroses, occurrence of this and other piroplasms in the two metapopulations was determined to assess possible risk. Blood specimens were collected from 156 black rhinoceroses: 80 from D. b. bicornis and 76 from D. b. minor. DNA was extracted; the V4 hypervariable region of the parasite 18S rRNA gene was amplified and subjected to the Reverse Line Blot (RLB) hybridization assay. There was a significant difference in occurrence of piroplasms: 18/80 (23%) in D. b. bicornis and 39/76 (51%) in D. b. minor. Theileria bicornis occurred in significantly more of the D. b. minor population (36/76; 47%) than the D. b. bicornis population (1/80; 1%); with B. bicornis the difference was not significant: D. b. bicornis 5/80 (6%) and D. b. minor 9/76 (11%). Three individuals were infected with Theileria equi. Results were confirmed using molecular characterization of the near full-length parasite 18S rRNA gene of 13 selected specimens. We identified four (Tb1, Tb2, Tb3 and Tb4) 18S rDNA sequence types for T. bicornis, two for B. bicornis (Bb1 and Bb2) and one for T. equi (Teq1). We furthermore identified T. bicornis haplotypes H1, H3 and H4 in 10 rhinoceroses; H3 was the most common haplotype identified. Rhinoceroses inhabiting more arid areas are apparently free of T. bicornis and B. bicornis, probably due to the absence or scarcity of vectors. When individuals are relocated for metapopulation management purposes, appropriate prophylactic action should be taken to minimise the risk of babesiosis, which could be fatal.
英文关键词	Black rhinoceros Babesia bicornis Theileria bicornis Theileria equi Metapopulation management Reintroduction
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000614467800005
WOS类目	Infectious Diseases ; Microbiology ; Parasitology
WOS研究方向	Infectious Diseases ; Microbiology ; Parasitology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351893
作者单位	[Zimmermann, David E.] Vet Wildlife Serv, South African Natl Pk, POB 110040,Hadison Pk, ZA-8306 Kimberley, South Africa; [Zimmermann, David E.; Penzhorn, Barend L.; Vorster, Ilse; Troskie, Milana; Oosthuizen, Marinda C.] Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, Ticks & Tick Borne Dis Res Programme, Private Bag X04, ZA-0110 Onderstepoort, South Africa; [Penzhorn, Barend L.] South African Natl Biodivers Inst, Natl Zool Garden, Pretoria, South Africa; [Penzhorn, Barend L.; Oosthuizen, Marinda C.] Univ Pretoria, Fac Vet Sci, Ctr Vet Wildlife Studies, Private Bag X04, ZA-0110 Onderstepoort, South Africa; [Oosthuizen, Marinda C.] Univ Pretoria, Fac Vet Sci, Res & Postgrad Studies, Private Bag X04, ZA-0110 Onderstepoort, South Africa
推荐引用方式 GB/T 7714	Zimmermann, David E.,Penzhorn, Barend L.,Vorster, Ilse,et al. Babesia bicornis, Theileria bicornis and Theileria equi in metapopulations of two black rhinoceros (Diceros bicornis) subspecies in South Africa and their potential impact on conservation[J],2021,12(2).
APA	Zimmermann, David E.,Penzhorn, Barend L.,Vorster, Ilse,Troskie, Milana,&Oosthuizen, Marinda C..(2021).Babesia bicornis, Theileria bicornis and Theileria equi in metapopulations of two black rhinoceros (Diceros bicornis) subspecies in South Africa and their potential impact on conservation.TICKS AND TICK-BORNE DISEASES,12(2).
MLA	Zimmermann, David E.,et al."Babesia bicornis, Theileria bicornis and Theileria equi in metapopulations of two black rhinoceros (Diceros bicornis) subspecies in South Africa and their potential impact on conservation".TICKS AND TICK-BORNE DISEASES 12.2(2021).