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DOI	10.1016/j.talanta.2021.122443
	Analytical method for the simultaneous determination of a broad range of opioids in influent wastewater: Optimization, validation and applicability to monitor consumption patterns
	Boogaerts, Tim; Quireyns, Maarten; Covaci, Adrian; De Loof, Hans; van Nuijs, Alexander L. N.
通讯作者	Boogaerts, T ; van Nuijs, ALN (corresponding author), Univ Antwerp, Toxicol Ctr, Univ Pl 1, B-2610 Antwerp, Belgium.
来源期刊	TALANTA
ISSN	0039-9140
EISSN	1873-3573
出版年	2021
卷号	232
英文摘要	Wastewater-based epidemiology (WBE) employs the analysis of human metabolic biomarkers in influent wastewater (IWW) to estimate community-wide exposure to xenobiotics (e.g. prescription opioids). The low ng/L range of concentrations of these biomarkers and the complex matrix composition pose bioanalytical challenges related to sample preparation and detection/quantification. Therefore, a sensitive analytical method for the detection and analysis of 19 opioid biomarkers was optimized and validated according to the European Medicines Agency guidelines. Oasis HLB cartridges were used for sample concentration and an Atlantis T3 column with gradient elution resulted in sufficient separation of the analytes. Absolute recoveries (RE) were highly reproducible and ranged between 50 and 93% with the exception of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP). The lower limit of quantification (LLOQ) ranged between 1 and 100 ng/L and was based on the analyte concentrations found in IWW. Process efficiency was acceptable for all biomarkers for which an isotope-labelled deuterated analogue was available. All biomarkers showed high benchtop stability with the exception of buprenorphine, EDDP, fentanyl and normorphine. Apart from buprenorphine and hydrocodone, all analytes under investigation were detected at least once above LLOQ levels in five locations in Belgium. The highest population-normalized mass loads were found for tramadol, O-desmethyltramadol and codeine. The proposed methodology was able to evaluate spatial differences in opioid use.
英文关键词	Wastewater-based epidemiology Opioids Mass-spectrometric analysis Validation Stability Process efficiency
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000658317000049
WOS关键词	TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; ILLICIT DRUGS ; CHEMICAL-ANALYSIS ; TEMPORAL TRENDS ; SURFACE-WATER ; EXPOSURE ; EPIDEMIOLOGY ; CITIES ; BIOMARKERS
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351878
作者单位	[Boogaerts, Tim; Quireyns, Maarten; Covaci, Adrian; van Nuijs, Alexander L. N.] Univ Antwerp, Toxicol Ctr, Univ Pl 1, B-2610 Antwerp, Belgium; [De Loof, Hans] Univ Antwerp, Lab Physiopharmacol, Univ Pl 1, B-2610 Antwerp, Belgium
推荐引用方式 GB/T 7714	Boogaerts, Tim,Quireyns, Maarten,Covaci, Adrian,et al. Analytical method for the simultaneous determination of a broad range of opioids in influent wastewater: Optimization, validation and applicability to monitor consumption patterns[J],2021,232.
APA	Boogaerts, Tim,Quireyns, Maarten,Covaci, Adrian,De Loof, Hans,&van Nuijs, Alexander L. N..(2021).Analytical method for the simultaneous determination of a broad range of opioids in influent wastewater: Optimization, validation and applicability to monitor consumption patterns.TALANTA,232.
MLA	Boogaerts, Tim,et al."Analytical method for the simultaneous determination of a broad range of opioids in influent wastewater: Optimization, validation and applicability to monitor consumption patterns".TALANTA 232(2021).