干旱区生态环境知识资源中心(Arid): Investigation of flavonoid expression and metabolite content patterns during seed formation of Artemisia sphaerocephala Krasch.

Arid

DOI	10.1017/S096025852100012X
	Investigation of flavonoid expression and metabolite content patterns during seed formation of Artemisia sphaerocephala Krasch.
	Li, Chengshuai; Zhang, Lijing; Niu, Decao; Nan, Shuzhen; Miao, Xiumei; Hu, Xiaowei; Fu, Hua
通讯作者	Zhang, LJ (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Engn Res Ctr Grassland Ind, Key Lab Grassland Livestock Ind Innovat,Minist Ed, Lanzhou 730020, Peoples R China.
来源期刊	SEED SCIENCE RESEARCH
ISSN	0960-2585
EISSN	1475-2735
出版年	2021
卷号	31 期号:2 页码:136-148
英文摘要	Flavonoids are a group of phenolic secondary metabolites in plants that have important physiological, ecological and economic value. In this study, using the desert plant Artemisia sphaerocephala Krasch. as the sample material, the content and components of the total flavonoids in its seeds at seven different developmental stages were determined. In addition, the genes involved in flavonoid metabolism were identified by full-length transcriptome sequencing (third-generation sequencing technology based on PacBio RS II). Their expression levels were analysed by RNA-seq short reading sequencing, to reveal the patterns and regulation mechanisms of flavonoid accumulation during seed development. The key results were as follows: the content of total flavonoids in mature seeds was 15.05 mg g(-1), including five subclasses: flavonols, chalcones, flavones, flavanones and proanthocyanidins, among which flavonols accounted for 45.78%. The period of rapid accumulation of flavonoids was 40-70 d following anthesis. The high expression of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL) and UDP-glucose:flavonoids 3-o-glucosyltransferase (UF3GT) promoted the accumulation of total flavonoids, while the high expression of flavonoids 3 '-hydroxylase (F3 ' H) and flavonols synthase (FLS) made flavanols the main component. Transcription factors such as the MYB-bHLH-WDR (MBW) complex and Selenium-binding protein (SBP) directly regulated the structural genes of flavonoid metabolism, while C2H2-type zinc finger (C2H2), Zinc-finger transcription factor (GATA), Dehydration-responsive element binding (DREB), Global Transcription factor Group E protein (GTE), Trihelix DNA-binding factors (Trihelix) and Phytochrome-interacting factor (PIF) indirectly promoted the synthesis of flavonoids through hormones such as brassinoidsteroids (BRs) and abscisic acid (ABA). These results provided valuable resources for the application of related genes in genetics and breeding.
英文关键词	Artemisia sphaerocephala Krasch flavonoid metabolism metabolite analysis seed development transcriptome sequencing
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000669061200008
WOS关键词	PHENOLIC-ACIDS ; BIOSYNTHETIC-PATHWAY ; INDUCED ANTHOCYANIN ; ECTOPIC EXPRESSION ; CHALCONE SYNTHASE ; EDIBLE FLOWERS ; GENES ; ACCUMULATION ; TRANSPORT ; PROANTHOCYANIDIN
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351756
作者单位	[Li, Chengshuai; Zhang, Lijing; Niu, Decao; Nan, Shuzhen; Miao, Xiumei; Hu, Xiaowei; Fu, Hua] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Engn Res Ctr Grassland Ind, Key Lab Grassland Livestock Ind Innovat,Minist Ed, Lanzhou 730020, Peoples R China
推荐引用方式 GB/T 7714	Li, Chengshuai,Zhang, Lijing,Niu, Decao,et al. Investigation of flavonoid expression and metabolite content patterns during seed formation of Artemisia sphaerocephala Krasch.[J]. 兰州大学,2021,31(2):136-148.
APA	Li, Chengshuai.,Zhang, Lijing.,Niu, Decao.,Nan, Shuzhen.,Miao, Xiumei.,...&Fu, Hua.(2021).Investigation of flavonoid expression and metabolite content patterns during seed formation of Artemisia sphaerocephala Krasch..SEED SCIENCE RESEARCH,31(2),136-148.
MLA	Li, Chengshuai,et al."Investigation of flavonoid expression and metabolite content patterns during seed formation of Artemisia sphaerocephala Krasch.".SEED SCIENCE RESEARCH 31.2(2021):136-148.