干旱区生态环境知识资源中心(Arid): Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

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DOI	10.1038/s41598-021-83556-8
	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
	Mohammed, Afrah E.; Sonbol, Hana; Alwakeel, Suaad Saleh; Alotaibi, Modhi O.; Alotaibi, Sohailah; Alothman, Nouf; Suliman, Rasha Saad; Ahmedah, Hanadi Talal; Ali, Rizwan
通讯作者	Mohammed, AE ; Sonbol, H ; Alwakeel, SS ; Alotaibi, MO (corresponding author), Princess Nourah Bint Abdulrahman Univ, Coll Sci, Dept Biol, Riyadh 84428, Saudi Arabia.
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2021
卷号	11 期号:1
英文摘要	Soil is considered an extensively explored ecological niche for microorganisms that produce useful biologically active natural products suitable for pharmaceutical applications. The current study aimed at investigating biological activities and metabolic profiles of three fungal strains identified from different desert sites in Saudi Arabia. Soil fungal isolates were collected from AlQasab, Tabuk, and Almuzahimiyah in Saudi Arabia and identified. Furthermore, their antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli in blood, nutrient, and Sabouraud dextrose agars. Moreover, fungal extracts were evaluated on cell viability/proliferation against human breast carcinoma and colorectal adenocarcinoma cells. To identify the biomolecules of the fungal extracts, High-performance liquid chromatography HPLC-DAD coupled to analytical LC-QTOF-MS method was employed for fungal ethyl acetate crude extract. Identified fungal isolates, Chaetomium sp. Bipolaris sp. and Fusarium venenatum showed varied inhibitory activity against tested microbes in relation to crude extract, microbial strain tested, and growth media. F. venenatum showed higher anticancer activity compared to Chaetomium sp. and Bipolaris sp. extracts against four of the tested cancer cell lines. Screening by HPLC and LC/MS-QTOF identified nine compounds from Chaetomium sp. and three from Bipolaris sp. however, for F. venenatum extracts compounds were not fully identified. In light of the present findings, some biological activities of fungal extracts were approved in vitro, suggesting that such extracts could be a useful starting point to find compounds that possess promising agents for medical applications. Further investigations to identify exact biomolecules from F. venenatum extracts are needed.
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000626627400008
WOS关键词	ENDOPHYTIC FUNGUS ; CHAETOMIUM-GLOBOSUM ; DESERT SOILS ; VERTICILLIUM-HEMIPTERIGENUM ; SECONDARY METABOLITES ; HALOPHILIC FUNGI ; NATURAL-PRODUCTS ; PLANT-EXTRACTS ; CELL-LINE ; CYTOTOXICITY
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351743
作者单位	[Mohammed, Afrah E.; Sonbol, Hana; Alwakeel, Suaad Saleh; Alotaibi, Modhi O.; Alotaibi, Sohailah; Alothman, Nouf] Princess Nourah Bint Abdulrahman Univ, Coll Sci, Dept Biol, Riyadh 84428, Saudi Arabia; [Suliman, Rasha Saad] King Saud Bin Abdulaziz Univ Hlth Sci, Coll Pharm, Pharmaceut Sci Dept, Riyadh, Saudi Arabia; [Suliman, Rasha Saad] King Abdullah Int Med Res Ctr, Riyadh, Saudi Arabia; [Ahmedah, Hanadi Talal] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Technol, Rabegh, Saudi Arabia; [Ali, Rizwan] King Saud Bin Abdulaziz Univ Hlth Sci, King Abdullah Int Med Res Ctr, Minist Natl Guard Hlth Res, Med Res Core Facil & Platforms, Riyadh 11481, Saudi Arabia
推荐引用方式 GB/T 7714	Mohammed, Afrah E.,Sonbol, Hana,Alwakeel, Suaad Saleh,et al. Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling[J],2021,11(1).
APA	Mohammed, Afrah E..,Sonbol, Hana.,Alwakeel, Suaad Saleh.,Alotaibi, Modhi O..,Alotaibi, Sohailah.,...&Ali, Rizwan.(2021).Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling.SCIENTIFIC REPORTS,11(1).
MLA	Mohammed, Afrah E.,et al."Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling".SCIENTIFIC REPORTS 11.1(2021).