干旱区生态环境知识资源中心(Arid): Transcriptome profiling provide new insights into the molecular mechanism of grapevine response to heat, drought, and combined stress

Arid

DOI	10.1016/j.scienta.2021.110076
	Transcriptome profiling provide new insights into the molecular mechanism of grapevine response to heat, drought, and combined stress
	Ju, Yan-lun; Min, Zhuo; Zhang, Yang; Zhang, Ke-kun; Liu, Min; Fang, Yu-lin
通讯作者	Fang, YL (corresponding author), Northwest A&F Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China. ; Liu, M (corresponding author), Shanxi Agr Univ, Coll Food Sci & Engn, Taigu 030801, Shanxi, Peoples R China.
来源期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
EISSN	1879-1018
出版年	2021
卷号	286
英文摘要	Grapes are a globally important fruit and usually grown in arid- and semi-arid areas. Investigating the molecular mechanism of drought and heat tolerance will provide important information for the improvement of grapevine response to drought and heat stresses. In the present study, one-year-old 'Cabernet Sauvignon' cuttings were subjected to drought (D), heat (H), and a combination of the two (HD), and RNA-seq was conducted using the Illumina Hiseq 4000 platform; 47.67-66.80 million clean reads were generated. In total, 2958, 1286, and 5057 differentially expressed genes (DEGs) were detected in the H, D, and HD groups, respectively. Based on the GO database, DEGs were annotated to 2732 (H), 2008 (D), and 3281 (HD) functional groups. A total of 733 (D), 1129 (H), and 1954 (HD) DEGs were annotated to 97, 107, and 121 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. Photosynthesis-related and hormone signal pathways were significantly regulated in these stresses. Many transcription factors (TFs), including MYBs, NACs, HSFs, and bHLH, played an important role in heat and drought grapevine stress. Notably, a total of 19 (H), 4 (D), and 36 (HD) DEGs were enriched in the spliceosome (vvi03040) pathway, suggesting that alternative splicing (AS) plays a key post-transcriptional regulatory role in response to the single and combination stressors. This study not only clarifies the mechanism underlying grapevine responses to heat and drought stress, but it also provides the candidate genes involved with these stressors.
英文关键词	Grapevine Drought Heat Transcriptome Molecular mechanism Alternative splicing
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000656083600001
WOS关键词	GENE-EXPRESSION ; ARABIDOPSIS ; ACCLIMATION ; COMBINATION ; PROTEINS ; LEAVES ; LEAF
WOS类目	Horticulture
WOS研究方向	Agriculture
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351692
作者单位	[Ju, Yan-lun; Zhang, Ke-kun; Fang, Yu-lin] Northwest A&F Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China; [Min, Zhuo; Zhang, Yang] Moutai Univ, Dept Brewing Engn, Renhuai 564507, Guizhou, Peoples R China; [Liu, Min] Shanxi Agr Univ, Coll Food Sci & Engn, Taigu 030801, Shanxi, Peoples R China; [Fang, Yu-lin] Northwest A&F Univ, Heyang Viti Viniculture Stn, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Ju, Yan-lun,Min, Zhuo,Zhang, Yang,et al. Transcriptome profiling provide new insights into the molecular mechanism of grapevine response to heat, drought, and combined stress[J]. 西北农林科技大学,2021,286.
APA	Ju, Yan-lun,Min, Zhuo,Zhang, Yang,Zhang, Ke-kun,Liu, Min,&Fang, Yu-lin.(2021).Transcriptome profiling provide new insights into the molecular mechanism of grapevine response to heat, drought, and combined stress.SCIENTIA HORTICULTURAE,286.
MLA	Ju, Yan-lun,et al."Transcriptome profiling provide new insights into the molecular mechanism of grapevine response to heat, drought, and combined stress".SCIENTIA HORTICULTURAE 286(2021).