干旱区生态环境知识资源中心(Arid): Cells responding to hedgehog signaling contribute to the theca of ovarian follicles

Arid

DOI	10.1530/REP-20-0471
	Cells responding to hedgehog signaling contribute to the theca of ovarian follicles
	Cowan, Robert G.; Quirk, Susan M.
通讯作者	Quirk, SM (corresponding author), Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA.
来源期刊	REPRODUCTION
ISSN	1470-1626
出版年	2021
卷号	161 期号:4 页码:437-448
英文摘要	Cell-fate mapping was used to identify cells that respond to the hedgehog (HH) signaling pathway and that are incorporated into the theca cell layer during ovarian follicle development. Expression of Gli1 is increased by HH signaling and can be used as a marker of cells responsive to HH in reporter mice. In transgenic Gli1ERcre/tdT mice, injection of tamoxifen (TAM) induces cre-mediated recombination and expression of td tomato (tdT) which leads to permanent fluorescent marking of cells expressing Gli1 and their progeny. The identity of tdT-positive cells was determined by co-staining ovaries for endothelial cells (CD31), pericytes (CSPG4), vascular smooth muscle cells (VSMC; smooth muscle actin) and steroidogenic cells (cytochrome P450 17A1). Gli1ERcre/tdT mice were injected with TAM on the day of birth. Cells positive for tdT in 2-day-old mice were identified as pericytes, located primarily in the medulla of the ovary in close proximity to endothelial cells. In both prepubertal mice and adult mice treated with equine chorionic gonadotropin to induce the formation of preovulatory follicles, tdT-positive cells were located within the theca cell layer and were identified as pericytes, VSMC and steroidogenic theca cells. Granulosa cells are known to express two HH ligands, Indian HH and desert HH (DHH). In DHHcre/tdT reporter mice, endothelial cells were marked as tdT-positive indicating that endothelial cells, in addition to granulosa cells, express Dhh in the ovary. These findings suggest that HH signaling may stimulate the development of the vasculature along with steroidogenic capacity of the theca layer during follicle development.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000640597900002
WOS类目	Developmental Biology ; Reproductive Biology
WOS研究方向	Developmental Biology ; Reproductive Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351565
作者单位	[Cowan, Robert G.; Quirk, Susan M.] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA
推荐引用方式 GB/T 7714	Cowan, Robert G.,Quirk, Susan M.. Cells responding to hedgehog signaling contribute to the theca of ovarian follicles[J],2021,161(4):437-448.
APA	Cowan, Robert G.,&Quirk, Susan M..(2021).Cells responding to hedgehog signaling contribute to the theca of ovarian follicles.REPRODUCTION,161(4),437-448.
MLA	Cowan, Robert G.,et al."Cells responding to hedgehog signaling contribute to the theca of ovarian follicles".REPRODUCTION 161.4(2021):437-448.