干旱区生态环境知识资源中心(Arid): From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru

Arid

DOI	10.1371/journal.pone.0250401
	From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru
	Romero, Pedro E.; Calla-Quispe, Erika; Castillo-Vilcahuaman, Camila; Yokoo, Mateo; Fuentes-Rivera, Hammerly Lino; Ramirez, Jorge L.; Ampuero, Andre; Ibanez, Alfredo J.; Wong, Paolo
通讯作者	Romero, PE (corresponding author), Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia, Dept Ciencias Biol & Fisiol, Lima, Peru. ; Wong, P (corresponding author), Univ Piura, Fac Med Humana, Dept Ciencias Med, Lima, Peru.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2021
卷号	16 期号:4
英文摘要	The Rimac river is the main source of water for Lima, Peru's capital megacity. The river is constantly affected by different types of contamination including mine tailings in the Andes and urban sewage in the metropolitan area. In this work, we aim to produce the first characterization of aquatic bacterial communities in the Rimac river using a 16S rRNA metabarcoding approach which would be useful to identify bacterial diversity and potential understudied pathogens. We report a lower diversity in bacterial communities from the Lower Rimac (Metropolitan zone) in comparison to other sub-basins. Samples were generally grouped according to their geographical location. Bacterial classes Alphaproteobacteria, Bacteroidia, Campylobacteria, Fusobacteriia, and Gammaproteobacteria were the most frequent along the river. Arcobacter cryaerophilus (Campylobacteria) was the most frequent species in the Lower Rimac while Flavobacterium succinicans (Bacteroidia) and Hypnocyclicus (Fusobacteriia) were the most predominant in the Upper Rimac. Predicted metabolic functions in the microbiota include bacterial motility and quorum sensing. Additional metabolomic analyses showed the presence of some insecticides and herbicides in the Parac-Upper Rimac and Santa Eulalia-Parac sub-basins. The dominance in the Metropolitan area of Arcobacter cryaerophilus, an emergent pathogen associated with fecal contamination and antibiotic multiresistance, that is not usually reported in traditional microbiological quality assessments, highlights the necessity to apply next-generation sequencing tools to improve pathogen surveillance. We believe that our study will encourage the integration of omics sciences in Peru and its application on current environmental and public health issues.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000644138300017
WOS关键词	ARCOBACTER ; DIVERSITY ; METABOLOMICS ; RESERVOIR ; DYNAMICS ; REVEALS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351398
作者单位	[Romero, Pedro E.; Castillo-Vilcahuaman, Camila] Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia, Dept Ciencias Biol & Fisiol, Lima, Peru; [Calla-Quispe, Erika; Fuentes-Rivera, Hammerly Lino; Ibanez, Alfredo J.] Pontificia Univ Catolica Peru, Inst Ciencias Omicas & Biotecnol Aplicada ICOBA, Lima, Peru; [Yokoo, Mateo; Wong, Paolo] Univ Piura, Fac Med Humana, Dept Ciencias Med, Lima, Peru; [Ramirez, Jorge L.] Univ Nacl Mayor San Marcos, Fac Ciencias Biol, Dept Biol Celular & Genet, Lima, Peru; [Ampuero, Andre] Univ Nacl Mayor San Marcos, Dept Malacol & Carcinol, Museo Hist Nat, Lima, Peru
推荐引用方式 GB/T 7714	Romero, Pedro E.,Calla-Quispe, Erika,Castillo-Vilcahuaman, Camila,et al. From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru[J],2021,16(4).
APA	Romero, Pedro E..,Calla-Quispe, Erika.,Castillo-Vilcahuaman, Camila.,Yokoo, Mateo.,Fuentes-Rivera, Hammerly Lino.,...&Wong, Paolo.(2021).From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru.PLOS ONE,16(4).
MLA	Romero, Pedro E.,et al."From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru".PLOS ONE 16.4(2021).