干旱区生态环境知识资源中心(Arid): A comprehensive examination of the lysine acetylation targets in paper mulberry based on proteomics analyses

Arid

DOI	10.1371/journal.pone.0240947
	A comprehensive examination of the lysine acetylation targets in paper mulberry based on proteomics analyses
	Li, Ping; Chen, Chao; Dong, Yibo
通讯作者	Chen, C (corresponding author), Guizhou Univ, Coll Anim Sci, Guiyang, Guizhou, Peoples R China.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2021
卷号	16 期号:3
英文摘要	Rocky desertification is a bottleneck that reduces ecological and environmental security in karst areas. Paper mulberry, a unique deciduous tree, shows good performance in rocky desertification areas. Its resistance mechanisms are therefore of high interest. In this study, a lysine acetylation proteomics analysis of paper mulberry seedling leaves was conducted in combination with the purification of acetylated protein by high-precision nano LC-MS/MS. We identified a total of 7130 acetylation sites in 3179 proteins. Analysis of the modified sites showed a predominance of nine motifs. Six positively charged residues: lysine (K), arginine (R), and histidine (H), serine (S), threonine (T), and tyrosine (Y) occurred most frequently at the +1 position, phenylalanine (F) was both detected both upstream and downstream of the acetylated lysines; and the sequence logos showed a strong preference for lysine and arginine around acetylated lysines. Functional annotation revealed that the identified enzymes were mainly involved in translation, transcription, ribosomal structure and biological processes, showing that lysine acetylation can regulate various aspects of primary carbon and nitrogen metabolism and secondary metabolism. Acetylated proteins were enriched in the chloroplast, cytoplasm, and nucleus, and many stress response-related proteins were also discovered to be acetylated, including PAL, HSP70, and ERF. HSP70, an important protein involved in plant abiotic and disease stress responses, was identified in paper mulberry, although it is rarely found in woody plants. This may be further examined in research in other plants and could explain the good adaptation of paper mulberry to the karst environment. However, these hypotheses require further verification. Our data can provide a new starting point for the further analysis of the acetylation function in paper mulberry and other plants.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000629590400011
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351388
作者单位	[Li, Ping; Chen, Chao; Dong, Yibo] Guizhou Univ, Coll Anim Sci, Guiyang, Guizhou, Peoples R China; [Li, Ping] Sichuan Acad Grassland Sci, Inst Grassland Res, Chengdu, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Li, Ping,Chen, Chao,Dong, Yibo. A comprehensive examination of the lysine acetylation targets in paper mulberry based on proteomics analyses[J],2021,16(3).
APA	Li, Ping,Chen, Chao,&Dong, Yibo.(2021).A comprehensive examination of the lysine acetylation targets in paper mulberry based on proteomics analyses.PLOS ONE,16(3).
MLA	Li, Ping,et al."A comprehensive examination of the lysine acetylation targets in paper mulberry based on proteomics analyses".PLOS ONE 16.3(2021).