干旱区生态环境知识资源中心(Arid): Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.)

Arid

DOI	10.3390/plants10030536
	Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.)
	Khan, Abdul Latif; Al-Harrasi, Ahmed; Numan, Muhammad; AbdulKareem, Noor Mazin; Mabood, Fazal; Al-Rawahi, Ahmed
通讯作者	Al-Harrasi, A (corresponding author), Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman. ; Mabood, F (corresponding author), Univ Swat, Inst Chem Sci, Khyber Pakhtunkhwa 19200, Pakistan.
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2021
卷号	10 期号:3
英文摘要	Phoenix dactylifera (date palm) is a well-known nutritious and economically important fruit tree found in arid regions of the Middle East and North Africa. Being diploid, it has extremely high divergence in gender, where sex differentiation in immature date palms (Phoenix dactylifera L.) has remained an enigma in recent years. Herein, new robust infrared (near-infrared reflectance spectroscopy (NIRS) and Fourier transform infrared attenuated total reflectance (FTIR/ATR)) and nuclear magnetic resonance (NMR) spectroscopy methods coupled with extensive chemometric analysis were used to identify the sex differentiation in immature date palm leaves. NIRS/FTIR reflectance and H-1-NMR profiling suggested that the signals of monosaccharides (glucose and fructose) and/or disaccharides (maltose and sucrose) play key roles in sex differentiation. The three kinds of spectroscopic data were clearly differentiated among known and unknown male and female leaves via principal component and partial least square discriminant analyses. Furthermore, sex-specific genes and molecular markers obtained from the lower halves of LG12 chromosomes showed enhanced transcript accumulation of mPdIRDP52, mPdIRDP50, and PDK101 in females compared with in males. The phylogeny showed that the mPdIRD033, mPdIRD031, and mPdCIR032 markers formed distinctive clades with more than 70% similarity in gender differentiation. The three robust analyses provide an alternative tool to differentiate sex in date palm trees, which offers a solution to the long-standing challenge of dioecism and could enhance in situ tree propagation programs.
英文关键词	Phoenix dactylifera NIR and FTIR spectroscopy PLS regression NMR chemometrics RT-PCR transcript accumulation sex
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000634080800001
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351353
作者单位	[Khan, Abdul Latif; Al-Harrasi, Ahmed; AbdulKareem, Noor Mazin; Al-Rawahi, Ahmed] Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; [Numan, Muhammad] Univ N Carolina, Dept Biol, Greensboro, NC 27402 USA; [Mabood, Fazal] Univ Swat, Inst Chem Sci, Khyber Pakhtunkhwa 19200, Pakistan
推荐引用方式 GB/T 7714	Khan, Abdul Latif,Al-Harrasi, Ahmed,Numan, Muhammad,et al. Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.)[J],2021,10(3).
APA	Khan, Abdul Latif,Al-Harrasi, Ahmed,Numan, Muhammad,AbdulKareem, Noor Mazin,Mabood, Fazal,&Al-Rawahi, Ahmed.(2021).Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.).PLANTS-BASEL,10(3).
MLA	Khan, Abdul Latif,et al."Spectroscopic and Molecular Methods to Differentiate Gender in Immature Date Palm (Phoenix dactylifera L.)".PLANTS-BASEL 10.3(2021).