干旱区生态环境知识资源中心(Arid): In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.)

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DOI	10.1016/j.plaphy.2021.05.047
	In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.)
	Abdolinejad, Ruhollah; Shekafandeh, Akhtar; Jowkar, Abolfazl
通讯作者	Shekafandeh, A ; Jowkar, A (corresponding author), Shiraz Univ, Coll Agr, Dept Hort, Rd Shiraz Isfahan,POB 65186-71441, Shiraz, Iran.
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
EISSN	1873-2690
出版年	2021
卷号	166 页码:191-202
英文摘要	Fig tree (Ficus carica L.) is a precious fruit tree in semi-arid and arid areas worldwide which has difficulties in its conventional breeding programs. This study was carried out to make new genotypes with superior features based on the ploidy induction method. Thus, in vitro tetraploidization in two fig cultivars, namely 'Sabz' and 'Torsh', was successfully established using shoot tip explants and colchicine as the antimitotic agent in MS medium. The flow cytometry and chromosome counting techniques were used to verify tetraploid plants. The results revealed that, in comparison to the original diploid plants of both cultivars, tetraploid plants significantly had taller stems, larger leaves, a greater number of chloroplasts in guard cells, and higher chlorophyll content and photosynthesis rate. UPLC-MS analysis revealed that the level of growth stimulator phytohormones, including ZR, IAA, GA(3), SA, and JA in the tetraploid plants of both cultivars were significantly higher than their diploid controls. In contrast, they had less accumulated growth inhibitor phytohormone (ABA) than their diploid explant source. Moreover, tetraploid plants had significantly accumulated a higher content of phenolic compounds, total soluble sugars, and total soluble proteins, but showed a significantly less total antioxidant activity. Consequently, it is concluded that the growth advantages of tetraploid figs created in this study are substantial in terms of phytohormonal, physiological, and phytochemical superiorities, as compared to their corresponding diploid plants. Polyploidization proves as a promising breeding tool for future breeding programs of the fig tree.
英文关键词	Chromosome doubling Ficus carica Flow cytometry Growth advantages Phytohormonal analysis
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000665139100017
WOS关键词	COMMON FIG ; POLYPLOIDY ; GROWTH ; ADAPTATION ; STRESS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351342
作者单位	[Abdolinejad, Ruhollah; Shekafandeh, Akhtar; Jowkar, Abolfazl] Shiraz Univ, Coll Agr, Dept Hort Sci, POB 65186-71441, Shiraz, Iran
推荐引用方式 GB/T 7714	Abdolinejad, Ruhollah,Shekafandeh, Akhtar,Jowkar, Abolfazl. In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.)[J],2021,166:191-202.
APA	Abdolinejad, Ruhollah,Shekafandeh, Akhtar,&Jowkar, Abolfazl.(2021).In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.).PLANT PHYSIOLOGY AND BIOCHEMISTRY,166,191-202.
MLA	Abdolinejad, Ruhollah,et al."In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.)".PLANT PHYSIOLOGY AND BIOCHEMISTRY 166(2021):191-202.