干旱区生态环境知识资源中心(Arid): Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts

Arid

DOI	10.1007/s11816-021-00674-5
	Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts
	Yin, Xiaoyue; Yan, Xia; Qian, Chaoju; Zhou, Shanshan; Fang, Tingzhou; Fan, Xingke; Gao, Yuan; Chang, Yuxiao; Yang, Jian; Ma, Xiao-Fei
通讯作者	Ma, XF (corresponding author), Chinese Acad Sci, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Dept Ecol & Agr Res, Northwest Inst Ecoenvironm & Resources, Lanzhou 730000, Gansu, Peoples R China. ; Ma, XF (corresponding author), Nantong Univ, Sch Life Sci, Nantong 226019, Jiangsu, Peoples R China.
来源期刊	PLANT BIOTECHNOLOGY REPORTS
ISSN	1863-5466
EISSN	1863-5474
出版年	2021
卷号	15 期号:3 页码:369-387
英文摘要	Agriophyllum squarrosum is a folk Mongolian medicine with pleiotropic pharmacological and ecological economic importance endemic to Asian temperature deserts. Terpenoids play critical roles in biotic and abiotic stresses due to their anti-oxidative activities. Based on non-targeted metabolomic analysis, we detected eight terpenoids enriched in the above-ground tissues of A. squarrosum, however, the molecular mechanism underlying terpenoids biosynthesis in this desert medicinal plant is rarely understood. Here, a comparative transcriptome analysis of different tissues in A. squarrosum was conducted to identify 84 unigenes encoding key enzymes in the upstream backbone biosynthesis and 53 unigenes encoding the downstream enzymes for terpenoid diversification. Most of the upstream genes exhibited significant high expression levels in leaf, and some of which were validated by qRT-PCR. Phylogenetic analysis showed that two downstream gene families OSCs (oxidosqualene cyclases) and TPSs (mainly in terpene synthases -g subfamily) had undergone notable gene expansions in A. squarrosum comparing with the other Amaranthaceae plant species and Arabidopsis. Nevertheless, most members from these two gene families showed the tissue-specific expression in A. squarrosum, which supported the diversification and tissue-specific enrichment of terpenoids across above-ground tissues. Considering to the habitat characteristics of A. squarrosum, we proposed that the enrichment of terpenoids and the functional diversification of terpenoids biosynthesis enzymes were more or less involved into its adaptation to stressful environments of deserts. These results expand the available genetic information underlying terpenoid biosynthesis in A. squarrosum, and contribute to deeper researches on pharmaceutical and eco-agricultural applications in this desert medicinal plant.
英文关键词	Agriophyllum squarrosum Transcriptome Terpenoid biosynthesis Oxidosqualene cyclases Terpene synthases Gene expansion
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000653691100001
WOS关键词	RNA-SEQ DATA ; CANDIDATE GENES ; EXPRESSION ANALYSIS ; SYNTHASE GENES ; PATHWAYS ; IDENTIFICATION ; TRITERPENES ; METABOLISM ; TOLERANCE ; DIVERSITY
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
来源机构	中国科学院西北生态环境资源研究院
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351331
作者单位	[Yin, Xiaoyue; Qian, Chaoju; Zhou, Shanshan; Fang, Tingzhou; Fan, Xingke; Ma, Xiao-Fei] Chinese Acad Sci, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Dept Ecol & Agr Res, Northwest Inst Ecoenvironm & Resources, Lanzhou 730000, Gansu, Peoples R China; [Yin, Xiaoyue; Zhou, Shanshan; Fang, Tingzhou; Fan, Xingke] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yan, Xia; Ma, Xiao-Fei] Nantong Univ, Sch Life Sci, Nantong 226019, Jiangsu, Peoples R China; [Gao, Yuan] Yibin Univ, Fac Mat & Chem Engn, Yibin 644000, Sichuan, Peoples R China; [Chang, Yuxiao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 450002, Peoples R China; [Yang, Jian] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Di Herbs, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Yin, Xiaoyue,Yan, Xia,Qian, Chaoju,et al. Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts[J]. 中国科学院西北生态环境资源研究院,2021,15(3):369-387.
APA	Yin, Xiaoyue.,Yan, Xia.,Qian, Chaoju.,Zhou, Shanshan.,Fang, Tingzhou.,...&Ma, Xiao-Fei.(2021).Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts.PLANT BIOTECHNOLOGY REPORTS,15(3),369-387.
MLA	Yin, Xiaoyue,et al."Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts".PLANT BIOTECHNOLOGY REPORTS 15.3(2021):369-387.