干旱区生态环境知识资源中心(Arid): Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate

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DOI	10.1016/j.phytochem.2021.112760
	Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate
	Trabelcy, Beny; Chinkov, Nicka; Samuni-Blank, Michal; Merav, Mayan; Izhaki, Ido; Carmeli, Shmuel; Gerchman, Yoram
通讯作者	Gerchman, Y (corresponding author), Univ Haifa, Fac Nat Sci, Dept Environm & Evolutionary Biol, IL-3498838 Haifa, Israel.
来源期刊	PHYTOCHEMISTRY
ISSN	0031-9422
EISSN	1873-3700
出版年	2021
卷号	187
英文摘要	Here we describe the structure elucidation and quantification of six glucosinolates (GSLs) from the roots of the desert plant Ochradenus baccatus, Delile 1813 (family Resedaceae; order Brassicales). The structure elucidation was established on the corresponding enzymatically desulfated derivatives of the native GSLs of the plant. Among these GSLs we describe the previously undescribed 2 ''-O-(alpha-L-arabinopyranosyloxy)benzylglucosinolate (1a), for which we propose the name glucoochradenin. The other five glucosinolates (2a-6a) were (2S)-2-hydroxy-2-phenylethylglucosinolate (2a; glucobarbarin), 2 ''-O-(alpha-L-rhamnopyranosyloxy)benzylglucosinolate (3a), benzylglucosinolate (4a; glucotropaeolin), indol-3-ylmethylglucosinolate (5a; glucobrassicin) and phenethylglucosinolate (6a; gluconasturtiin), all elucidated as their desulfo-derivatives, 2b-6b respectively). Structures were elucidated by MS and 1D and 2D-NMR techniques, the identity of the arabinose verified by ion chromatography, and the absolute configuration of the sugar units determined by hydrolysis, coupling to cysteine methyl-ester and phenyl isothiocyanate followed by HPLC-MS analysis of the resulted diastereomers. Response factors were generated for desulfo-2 ''-O-(alpha-L-arabinopyranosyloxy)benzylglucosinolate and for desulfo-2 ''-O(alpha-L-rhamnopyranosyloxy)benzylglucosinolate and all six GSLs were quantified, indicating that the root of O. baccatus is rich in GSLs (Avg. 61.3 +/- 10.0 mu mol/g DW and up to 337.2 mu mol/g DW).
英文关键词	Ochradenus baccatus (Resedaceae) Desert plants Root Aromatic glucosinolates Glycosylated glucosinolates Structure elucidation NMR Quantitative variation Response factors
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000654130400012
WOS关键词	ANTIMICROBIAL ACTIVITY ; ISOTHIOCYANATES ; IDENTIFICATION ; DIVERSITY ; ROOT ; HORSERADISH ; SEEDS
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351322
作者单位	[Trabelcy, Beny; Samuni-Blank, Michal; Izhaki, Ido; Gerchman, Yoram] Univ Haifa, Fac Nat Sci, Dept Environm & Evolutionary Biol, IL-3498838 Haifa, Israel; [Chinkov, Nicka] Univ Haifa, Fac Nat Sci, Dept Biol & Environm, Campus Oranim, IL-36006 Qiryat Tivon, Israel; [Merav, Mayan; Carmeli, Shmuel] Tel Aviv Univ, Raymond & Beverly Sackler Sch Chem, IL-69978 Tel Aviv, Israel; [Merav, Mayan; Carmeli, Shmuel] Tel Aviv Univ, Fac Exact Sci, IL-69978 Tel Aviv, Israel; [Gerchman, Yoram] Oranim Coll, Campus Oranim, IL-36006 Qiryat Tivon, Israel
推荐引用方式 GB/T 7714	Trabelcy, Beny,Chinkov, Nicka,Samuni-Blank, Michal,et al. Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate[J],2021,187.
APA	Trabelcy, Beny.,Chinkov, Nicka.,Samuni-Blank, Michal.,Merav, Mayan.,Izhaki, Ido.,...&Gerchman, Yoram.(2021).Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate.PHYTOCHEMISTRY,187.
MLA	Trabelcy, Beny,et al."Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate".PHYTOCHEMISTRY 187(2021).