干旱区生态环境知识资源中心(Arid): Prevalence and Antimicrobial Susceptibility Profile of Mycobacterium bovis Isolated from Egyptian Buffaloes' Mastitic Milk

Arid

DOI	10.17582/journal.pjz/20200417180418
	Prevalence and Antimicrobial Susceptibility Profile of Mycobacterium bovis Isolated from Egyptian Buffaloes' Mastitic Milk
	Khalifa, Eman; Khalil, Riad; Elaadli, Haitham
通讯作者	Khalifa, E (corresponding author), Matrouh Univ, Dept Microbiol, Fac Vet Med, Matrouh, Egypt.
来源期刊	PAKISTAN JOURNAL OF ZOOLOGY
ISSN	0030-9923
出版年	2021
卷号	53 期号:3 页码:1189-1191
英文摘要	From the world's highlight on dangers of tuberculosis as a zoonotic and debilitating disease threatening animal and human health beside economy, this research aimed to determine the prevalence of Mycobacterium bovis from buffaloes' mastitic milk by isolation on Lowenstein-Jensen medium (LJ medium), Ziehl-Neelsen staining; identification by different biochemical tests and polymerase chain reaction (PCR) at 500bp diagnostic for M. bovis, as well as (2) to determine the therapeutic efficacy of the various antimicrobials (9 types) on the isolated M. bovis by using different antimicrobial sensitivity plate method. A total number of 100 samples of mastitic milk from native lactating buffaloes' breed were collected aseptically from 4 private farms in Cairo-Alexandria desert road in the north part of the Egypt and subjected to bacteriological and molecular examination for M. bovis followed by their antibiogram profile. Seven (7%) isolates were identified as M. bovis from totally examined 100 milk samples and isolated M. bovis showed resistance to ciprofloxacin; gentamycin and sulphamethoxazole-trimethoprim while were intermediate sensitive to both of erythromycin and norfloxacin but on the contrary were sensitive to amikacin; cefotaxime; clindamycin and streptomycin. It can be concluded that M. bovis can be the causative agent of mastitis in Egyptian Buffaloes and could be a potential risk for zoonotic transmission to man as well as economic losses. So, strict hygienic regulations and novel diagnostic tools should be used for prevention and detection of tuberculous mastitis in buffaloes' farms. The general public health should also be intensely warned from consuming raw or unpasteurized milk. All these prophylactic measures will eventually lead to a positive impact on public health.
英文关键词	Mycobacterium bovis Buffaloes Mastitis Isolation PCR Antimicrobials
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000640497800019
WOS类目	Zoology
WOS研究方向	Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351266
作者单位	[Khalifa, Eman] Matrouh Univ, Dept Microbiol, Fac Vet Med, Matrouh, Egypt; [Khalil, Riad] Alexandria Univ, Dept Poultry & Fish Dis, Fac Vet Med, Alexandria, Egypt; [Elaadli, Haitham] Alexandria Univ, Dept Anim Hyg & Zoonoses, Fac Vet Med, Alexandria, Egypt
推荐引用方式 GB/T 7714	Khalifa, Eman,Khalil, Riad,Elaadli, Haitham. Prevalence and Antimicrobial Susceptibility Profile of Mycobacterium bovis Isolated from Egyptian Buffaloes' Mastitic Milk[J],2021,53(3):1189-1191.
APA	Khalifa, Eman,Khalil, Riad,&Elaadli, Haitham.(2021).Prevalence and Antimicrobial Susceptibility Profile of Mycobacterium bovis Isolated from Egyptian Buffaloes' Mastitic Milk.PAKISTAN JOURNAL OF ZOOLOGY,53(3),1189-1191.
MLA	Khalifa, Eman,et al."Prevalence and Antimicrobial Susceptibility Profile of Mycobacterium bovis Isolated from Egyptian Buffaloes' Mastitic Milk".PAKISTAN JOURNAL OF ZOOLOGY 53.3(2021):1189-1191.