Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1038/s41522-021-00209-4 |
| Experimental parameters defining ultra-low biomass bioaerosol analysis | |
| Luhung, Irvan; Uchida, Akira; Lim, Serene B. Y.; Gaultier, Nicolas E.; Kee, Carmon; Lau, Kenny J. X.; Gusareva, Elena S.; Heinle, Cassie E.; Wong, Anthony; Premkrishnan, Balakrishnan N., V; Purbojati, Rikky W.; Acerbi, Enzo; Kim, Hie Lim; Junqueira, Ana C. M.; Longford, Sharon; Lohar, Sachin R.; Yap, Zhei Hwee; Panicker, Deepa; Koh, Yanqing; Kushwaha, Kavita K.; Ang, Poh Nee; Putra, Alexander; Drautz-Moses, Daniela, I; Schuster, Stephan C. | |
| 通讯作者 | Schuster, SC (corresponding author), Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn SCELSE, Singapore, Singapore. |
| 来源期刊 | NPJ BIOFILMS AND MICROBIOMES
 |
| EISSN | 2055-5008 |
| 出版年 | 2021 |
| 卷号 | 7期号:1 |
| 英文摘要 | Investigation of the microbial ecology of terrestrial, aquatic and atmospheric ecosystems requires specific sampling and analytical technologies, owing to vastly different biomass densities typically encountered. In particular, the ultra-low biomass nature of air presents an inherent analytical challenge that is confounded by temporal fluctuations in community structure. Our ultra-low biomass pipeline advances the field of bioaerosol research by significantly reducing sampling times from days/weeks/months to minutes/hours, while maintaining the ability to perform species-level identification through direct metagenomic sequencing. The study further addresses all experimental factors contributing to analysis outcome, such as amassment, storage and extraction, as well as factors that impact on nucleic acid analysis. Quantity and quality of nucleic acid extracts from each optimisation step are evaluated using fluorometry, qPCR and sequencing. Both metagenomics and marker gene amplification-based (16S and ITS) sequencing are assessed with regard to their taxonomic resolution and inter-comparability. The pipeline is robust across a wide range of climatic settings, ranging from arctic to desert to tropical environments. Ultimately, the pipeline can be adapted to environmental settings, such as dust and surfaces, which also require ultra-low biomass analytics. |
| 类型 | Article |
| 语种 | 英语 |
| 开放获取类型 | gold, Green Published |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000641749500003 |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/351221 |
| 作者单位 | [Luhung, Irvan; Uchida, Akira; Lim, Serene B. Y.; Gaultier, Nicolas E.; Kee, Carmon; Lau, Kenny J. X.; Gusareva, Elena S.; Heinle, Cassie E.; Wong, Anthony; Premkrishnan, Balakrishnan N., V; Purbojati, Rikky W.; Acerbi, Enzo; Kim, Hie Lim; Junqueira, Ana C. M.; Longford, Sharon; Lohar, Sachin R.; Yap, Zhei Hwee; Panicker, Deepa; Koh, Yanqing; Kushwaha, Kavita K.; Ang, Poh Nee; Putra, Alexander; Drautz-Moses, Daniela, I; Schuster, Stephan C.] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn SCELSE, Singapore, Singapore; [Junqueira, Ana C. M.] Univ Fed Rio de Janeiro, Dept Genet, Inst Biol, BR-21941590 Rio De Janeiro, Brazil |
| 推荐引用方式 GB/T 7714 | Luhung, Irvan,Uchida, Akira,Lim, Serene B. Y.,et al. Experimental parameters defining ultra-low biomass bioaerosol analysis[J],2021,7(1). |
| APA | Luhung, Irvan.,Uchida, Akira.,Lim, Serene B. Y..,Gaultier, Nicolas E..,Kee, Carmon.,...&Schuster, Stephan C..(2021).Experimental parameters defining ultra-low biomass bioaerosol analysis.NPJ BIOFILMS AND MICROBIOMES,7(1). |
| MLA | Luhung, Irvan,et al."Experimental parameters defining ultra-low biomass bioaerosol analysis".NPJ BIOFILMS AND MICROBIOMES 7.1(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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