干旱区生态环境知识资源中心(Arid): Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp.

Arid

DOI	10.1128/mSphere.00040-21
	Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp.
	Keppler, Emily A. Higgins; Mead, Heather L.; Barker, Bridget M.; Bean, Heather D.
通讯作者	Bean, HD (corresponding author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 USA. ; Bean, HD (corresponding author), Arizona State Univ, Biodesign Inst, Ctr Fundamental & Appl Microbi, Tempe, AZ 85281 USA.
来源期刊	MSPHERE
EISSN	2379-5042
出版年	2021
卷号	6 期号:2
英文摘要	Valley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative agents of Valley fever are the dimorphic fungi Coccidioides immitis and C. posadasii, which grow as mycelia in the environment and as spherules within the lungs of vulnerable hosts. Current diagnostics for Valley fever are severely lacking due to poor sensitivity and invasiveness, contributing to a 23-day median time to diagnosis, and therefore, new diagnostic tools are needed. We are working toward the development of a breath-based diagnostic for coccidioidomycosis, and in this initial study, we characterized the volatile metabolomes (or volatilomes) of in vitro cultures of Coccidioides. Using solid-phase microextraction (SPME) and comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GCxGC-TOFMS), we characterized the volatile organic compounds (VOCs) produced by six strains of each species during mycelial or spherule growth. We detected a total of 353 VOCs that were at least 2 fold more abundant in a Coccidioides culture than in medium controls and found that the volatile metabolome of Coccidioides is more dependent on the growth phase (spherules versus mycelia) than on the species. The volatile profiles of C. immitis and C. posadasii have strong similarities, indicating that a single suite of Valley fever breath biomarkers can be developed to detect both species. IMPORTANCE Coccidioidomycosis, or Valley fever, causes up to 30% of community acquired pneumonias in highly populated areas of the U.S. desert southwest where the disease is endemic. The infection is difficult to diagnose by standard serological and histopathological methods, which delays appropriate treatment. Therefore, we are working toward the development of breath-based diagnostics for Valley fever. In this study, we characterized the volatile metabolomes (or volatilomes) of six strains each of Coccidioides immitis and C. posadasii, the dimorphic fungal species that cause Valley fever. By analyzing the volatilomes during the two modes of growth of the fungus-mycelia and spherules-we observed that the life cycle plays a signifi- cant role in the volatiles produced by Coccidioides. In contrast, we observed no significant differences in the C. immitis versus C. posadasii volatilomes. These data suggest that life cycle, rather than species, should guide the selection of putative biomarkers for a Valley fever breath test.
英文关键词	GCxGC Valley fever biomarkers coccidioidomycosis comprehensive two-dimensional gas chromatography dimorphic fungus fungal infections untargeted metabolomics volatile metabolites
类型	Article
语种	英语
开放获取类型	Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000668654200005
WOS关键词	ORGANIC-COMPOUNDS MVOCS ; ASPERGILLUS-FUMIGATUS ; IN-VITRO ; BREATH ; SOIL ; 2-PENTYLFURAN ; POPULATION ; WASHINGTON ; POSADASII ; RANGE
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	Arizona State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351175
作者单位	[Keppler, Emily A. Higgins; Bean, Heather D.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 USA; [Keppler, Emily A. Higgins; Bean, Heather D.] Arizona State Univ, Biodesign Inst, Ctr Fundamental & Appl Microbi, Tempe, AZ 85281 USA; [Mead, Heather L.; Barker, Bridget M.] No Arizona Univ, Pathogen & Microbiome Inst, Flagstaff, AZ 86011 USA
推荐引用方式 GB/T 7714	Keppler, Emily A. Higgins,Mead, Heather L.,Barker, Bridget M.,et al. Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp.[J]. Arizona State University,2021,6(2).
APA	Keppler, Emily A. Higgins,Mead, Heather L.,Barker, Bridget M.,&Bean, Heather D..(2021).Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp..MSPHERE,6(2).
MLA	Keppler, Emily A. Higgins,et al."Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp.".MSPHERE 6.2(2021).