干旱区生态环境知识资源中心(Arid): Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application

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DOI	10.3390/molecules26072091
	Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application
	Mohammed, Mostafa S.; Hefnawy, Mohamed M.; Al-Majed, Abdulrhman A.; Alrabiah, Haitham K.; Algrain, Nasser A.; Obaidullah, Ahmad J.; Altamimi, Abdulmalik S.; Bin Jardan, Yousef A.; Al-Hossaini, Abdullah M.
通讯作者	Hefnawy, MM (corresponding author), King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, POB 2457, Riyadh 11451, Saudi Arabia. ; Hefnawy, MM (corresponding author), Mansoura Univ, Fac Pharm, Dept Analyt Chem, Mansoura 35516, Egypt.
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2021
卷号	26 期号:7
英文摘要	A novel, fast and sensitive enantioselective HPLC assay with a new core-shell isopropyl carbamate cyclofructan 6 (superficially porous particle, SPP) chiral column (LarihcShell-P, LSP) was developed and validated for the enantiomeric separation and quantification of verapamil (VER) in rat plasma. The polar organic mobile phase composed of acetonitrile/methanol/trifluoroacetic acid/triethylamine (98:2:0.05: 0.025, v/v/v/v) and a flow rate of 0.5 mL/min was applied. Fluorescence detection set at excitation/emission wavelengths 280/313 nm was used and the whole analysis process was within 3.5 min, which is 10-fold lower than the previous reported HPLC methods in the literature. Propranolol was selected as the internal standard. The S-(-)- and R-(+)-VER enantiomers with the IS were extracted from rat plasma by utilizing Waters Oasis HLB C18 solid phase extraction cartridges without interference from endogenous compounds. The developed assay was validated following the US-FDA guidelines over the concentration range of 1-450 ng/mL (r(2) >= 0.997) for each enantiomer (plasma) and the lower limit of quantification was 1 ng/mL for both isomers. The intra- and inter-day precisions were not more than 11.6% and the recoveries of S-(-)- and R-(+)-VER at all quality control levels ranged from 92.3% to 98.2%. The developed approach was successfully applied to the stereoselective pharmacokinetic study of VER enantiomers after oral administration of 10 mg/kg racemic VER to Wistar rats. It was found that S-(-)-VER established higher C-max and area under the concentration-time curve (AUC) values than the R-(+)-enantiomer. The newly developed approach is the first chiral HPLC for the enantiomeric separation and quantification of verapamil utilizing a core-shell isopropyl carbamate cyclofructan 6 chiral column in rat plasma within 3.5 min after solid phase extraction (SPE).
英文关键词	verapamil enantiomers core– shell chiral column chiral HPLC rat plasma stereoselective pharmacokinetics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000638737200001
WOS关键词	PARTICLES
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351168
作者单位	[Mohammed, Mostafa S.; Hefnawy, Mohamed M.; Al-Majed, Abdulrhman A.; Alrabiah, Haitham K.; Algrain, Nasser A.; Obaidullah, Ahmad J.; Al-Hossaini, Abdullah M.] King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, POB 2457, Riyadh 11451, Saudi Arabia; [Mohammed, Mostafa S.] Natl Org Drug Control & Res, POB 29, Cairo, Egypt; [Hefnawy, Mohamed M.] Mansoura Univ, Fac Pharm, Dept Analyt Chem, Mansoura 35516, Egypt; [Altamimi, Abdulmalik S.] Prince Sattam Bin Abdulaziz Univ, Coll Pharm, Dept Pharmaceut Chem, Al Kharj 11942, Saudi Arabia; [Bin Jardan, Yousef A.] King Saud Univ, Coll Pharm, Dept Pharmaceut, POB 2457, Riyadh 11451, Saudi Arabia
推荐引用方式 GB/T 7714	Mohammed, Mostafa S.,Hefnawy, Mohamed M.,Al-Majed, Abdulrhman A.,et al. Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application[J]. King Saud University,2021,26(7).
APA	Mohammed, Mostafa S..,Hefnawy, Mohamed M..,Al-Majed, Abdulrhman A..,Alrabiah, Haitham K..,Algrain, Nasser A..,...&Al-Hossaini, Abdullah M..(2021).Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application.MOLECULES,26(7).
MLA	Mohammed, Mostafa S.,et al."Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application".MOLECULES 26.7(2021).