干旱区生态环境知识资源中心(Arid): Optimization of Pressurized Liquid Extraction of Lycopodiaceae Alkaloids Obtained from Two Lycopodium Species

Arid

DOI	10.3390/molecules26061626
	Optimization of Pressurized Liquid Extraction of Lycopodiaceae Alkaloids Obtained from Two Lycopodium Species
	Dymek, Aleksandra; Widelski, Jaroslaw; Wojtanowski, Krzysztof Kamil; Ploszaj, Paulina; Zhuravchak, Rostyslav; Mroczek, Tomasz
通讯作者	Dymek, A (corresponding author), Med Univ Lublin, Independent Lab Chem Nat Prod, 1 Chodzki St, PL-20093 Lublin, Poland.
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2021
卷号	26 期号:6
英文摘要	Alkaloids of the Lycopodiaceae family are of great interest to researchers due to their numerous properties and wide applications in medicine. They play a very important role mainly due to their potent antioxidant, antidepressant effects and a reversible ability to inhibit acetylcholinesterase (AChE) enzyme activity. This property is of immense importance due to the growing problem of an increasing number of patients with neurodegenerative diseases in developed countries and a lack of effective and efficient treatment for them. Numerous studies have shown that Lycopodiaceae alkaloids are a rich source of AChE inhibitors. In the obtaining of new therapeutic phytochemicals from plant material, the extraction process and its efficiency is crucial. Therefore, the aim of this work was to optimize the conditions of modern PLE to obtain bioactive alkaloids from two Lycopodium species: L. clavatum L. and L. annotinum L. Five different solvents of different polarity were used for prepared plant extracts in order to compare the alkaloid content in and thereby effectiveness of the entire extraction. PLE parameters were used based on multiple studies conducted that gave the highest alkaloids recovery. Crude extracts were purified using solid-phase extraction (SPE) on Oasis HLB cartridge and examined by HPLC/ESI-QTOF-MS of the highly abundant alkaloids. To the best of our knowledge, this is the first time such high recoveries have been obtained for known Lycopodiaceae alkaloids. The best extraction results of alkaloid-lycopodine were detected in the dichloromethane extract from L. clavatum, where the yield exceeded 45%. The high recovery of annotinine above 40% presented in L. annotinum was noticed in dichloromethane and ethyl acetate extracts. Moreover, chromatograms were obtained with all isolated alkaloids and the best separation and quality of the bands in methanolic extracts. Interestingly, no alkaloid amounts were detected in cyclohexane extracts belonging to the non-polar solvent. These results could be helpful for understanding and optimizing the best conditions for isolating potent AChE inhibitors.
英文关键词	Lycopodium clavatum L Lycopodium annotinum L Lycopodium alkaloids pressurized liquid extraction solid-phase extraction HPLC/ESI-QTOF-MS
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000645409600001
WOS关键词	PROTECTIVE POTENTIALS ; ACETYLCHOLINESTERASE ; HUPERZINE ; CLAVATUM
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351166
作者单位	[Dymek, Aleksandra; Ploszaj, Paulina; Mroczek, Tomasz] Med Univ Lublin, Independent Lab Chem Nat Prod, 1 Chodzki St, PL-20093 Lublin, Poland; [Widelski, Jaroslaw; Wojtanowski, Krzysztof Kamil] Med Univ Lublin, Dept Pharmacognosy, 1 Chodzki St, PL-20093 Lublin, Poland; [Zhuravchak, Rostyslav] Nat Boundary Rozvylka, Rivnenskyi Nat Reserve, UA-34500 Sarny, Rivne Region, Ukraine
推荐引用方式 GB/T 7714	Dymek, Aleksandra,Widelski, Jaroslaw,Wojtanowski, Krzysztof Kamil,et al. Optimization of Pressurized Liquid Extraction of Lycopodiaceae Alkaloids Obtained from Two Lycopodium Species[J],2021,26(6).
APA	Dymek, Aleksandra,Widelski, Jaroslaw,Wojtanowski, Krzysztof Kamil,Ploszaj, Paulina,Zhuravchak, Rostyslav,&Mroczek, Tomasz.(2021).Optimization of Pressurized Liquid Extraction of Lycopodiaceae Alkaloids Obtained from Two Lycopodium Species.MOLECULES,26(6).
MLA	Dymek, Aleksandra,et al."Optimization of Pressurized Liquid Extraction of Lycopodiaceae Alkaloids Obtained from Two Lycopodium Species".MOLECULES 26.6(2021).